European Journal of Chemistry

Micellar high performance liquid chromatographic determination of tinidazole in combination with ciprofloxacin or norfloxacin in bulk, pharmaceutical dosage forms and in spiked human plasma



Main Article Content

Mohamed Rizk
Safaa Shafik Toubar
Maha Mahmoud Abou El‐Alamin
Marwa Mohamed Mahmoud Azab

Abstract

A micellar liquid chromatographic method was developed for determination of tinidazole in bulk, dosage forms and human plasma using intersil cyano column and a mobile phase of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, 0.3% triethylamine in 0.02 M orthophosphoric acid:water (60:40, v:v) (pH = 4). The U.V. detection was achieved at 311 nm. Various chromatographic parameters were investigated to select the optimum conditions for the separation, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc. The method was linear over the concentration range 40-200 µg/mL with regression coefficient 0.999. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as stability-indicating method for determination of tinidazole under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters.


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Rizk, M.; Toubar, S. S.; El‐Alamin, M. M. A.; Azab, M. M. M. Micellar High Performance Liquid Chromatographic Determination of Tinidazole in Combination With Ciprofloxacin or Norfloxacin in Bulk, Pharmaceutical Dosage Forms and in Spiked Human Plasma. Eur. J. Chem. 2014, 5, 439-445.

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References

[1]. Palacin, C.; Tarrago, C.; Ortiz, J. A. Int. J. Gynecol. Obstet. 2000, 71, 37-46.
http://dx.doi.org/10.1016/S0020-7292(00)00351-9

[2]. Sweetman, S. Martindale, The Complete Drug Reference, 36th edition, The Pharmaceutical Press, London, 2009.

[3]. The British Pharmacopeia, The stationary office, London, 2012 (Electronic version).

[4]. The United States Pharmacopeia 30th edition and the National Formulary 25th edition, Rockville, MD, USA, 2007, (Electronic version).

[5]. European Pharmacopeia Commission, 5th edition, Supplement 5. 2, Council of Europe, Strasbourg, 2005.

[6]. Adegoke, O. A.; Umoh, O. E. J. Acta Pharm. 2009, 59, 407-419.
http://dx.doi.org/10.2478/v10007-009-0039-2

[7]. Mohammad, M. A.; Zawilla, N.; El-Anwar, F.; El-Moghazy, A. Chem. Pharm. Bull. 2007, 55, 1-6.
http://dx.doi.org/10.1248/cpb.55.1

[8]. Mohamed, M.; El-Gendy, A.; El-Bardicy, M.; Tawakkol, M.; Ahmad, A. J. Spectrosc. Lett. 1996, 29, 299-319.
http://dx.doi.org/10.1080/00387019608001604

[9]. Wang, J. Z.; Liu, B.; Sun, X. Q. Fenxi Ceshi Xuebao 2004, 23, 76-79.

[10]. Elokely, K. M.; Eldawy, M. A.; Elkersh, M. A.; El-Moselhy, T. F. Int. J. Anal. Chem. 2011, 2011, Article ID: 840178.

[11]. Chenghang, W. E.; Fang, W.; Chunya, L. J. Pharm. Biomed. Anal. 2006, 41, 1396-1400.
http://dx.doi.org/10.1016/j.jpba.2006.02.040

[12]. Merino, M.; Nunez-Vergara, L.; Squella. J. Electroanaly. 1999, 11, 1285-1292.
http://dx.doi.org/10.1002/(SICI)1521-4109(199911)11:17<1285::AID-ELAN1285>3.0.CO;2-T

[13]. Bakshi, M.; Singh, S. J. Pharm. Biomed. Anal. 2004, 34, 11-18.
http://dx.doi.org/10.1016/j.japna.2003.08.003

[14]. Argekar, A.; Powar, S. Indian Drugs 1999, 36, 399-402.

[15]. Tendolkar, N.; Desai, B.; Gaudh, J.; Shinde, V. Anal. Lett. 1995, 28, 1641-1653.
http://dx.doi.org/10.1080/00032719508002771

[16]. Argekar, A.; Kapadia, S.; Raj, S. Anal. Lett. 1996, 29, 1539-1549.
http://dx.doi.org/10.1080/00032719608001503

[17]. Armstrong, D.; Henry, S. J. Liq. Chromatogr. Relat. Technol. 1980, 3, 657-662.
http://dx.doi.org/10.1080/01483918008060181

[18]. Rizk, M.; Toubar, S. S.; El-Alamin, M. M. A.; Azab, M. M. M. Eur. J. Chem. 2014, 5(1), 11-17
http://dx.doi.org/10.5155/eurjchem.5.1.11-17.885

[19]. Esteve-Romero, J.; Canda-Broch, S.; Gil-Agusti, M.; Capella-Peiro, M.; Bose, D. Trends. Anal. Chem. 2005, 24, 75-91.
http://dx.doi.org/10.1016/j.trac.2004.11.003

[20]. Habel, D.; Guermouche, S.; Guermouche, M. J. Biomed. Chromatogr. 1997, 11, 16-18.
http://dx.doi.org/10.1002/(SICI)1099-0801(199701)11:1<16::AID-BMC619>3.0.CO;2-K

[21]. Validation of Analytical Procedure: Text and Methodology. International Conference on Harmonization Q2(R1), 2005 (http://www.bioforum.org.il/Uploads/Editor/karen/q2_r1_step4.pdf).

[22]. Miller, J. C.; Miller, J. N. Statistics and Chemometrics for Analytical Chemistry, 5th edition, Pearson Education Limited: Harlow, England, 2005, pp. 39-73, pp. 107-149, pp. 256.

[23]. Prasad, C. V.; Bharadwaj, V.; Parimoo, P. J. Pharm. Sci. 1996, 2, 255-258.

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