European Journal of Chemistry

Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus



Main Article Content

Sayed Abdel Kader Ahmed
Mahmoud Al-Refai

Abstract

The phytochemical investigation of the ethanolic extract of the aerial parts of Melilotus indicus resulted in the isolation of eight known flavonoids, namely; catechin (1), epicatechin (2), taxifolin (3), quercetin (4), quercetin 3-O-β-D-glucopyranoside (5), and quercetin 3-O-α-L-rhamnopyranoside (6), isorhamnetin 3-O-β-D-glucopyranoside (7) and isorhamnetin 3-O-β-D-rutinoside (8). These compounds have never been isolated from this species. The cytotoxic activities of the extracts of Melilotus indicus was evaluated by MTT assay against six cancer cell lines using doxorubicin as standard drug. The chloroform extract was the most potent cytotoxic agent against HepG-2cells with an IC50: 9.21 µg/mL.


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Ahmed, S. A. K.; Al-Refai, M. Chemical Constituents and Cytotoxic Activities of the Extracts of Melilotus Indicus. Eur. J. Chem. 2014, 5, 503-506.

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References

[1]. Sprent, J. I. Perspect. Plant Ecol. 1999, 2, 149-162.
http://dx.doi.org/10.1078/1433-8319-00068

[2]. Webb, C. J.; Sykes, W. R.; Garnock-Jones, P. J. Flora of New Zealand. Vol. IV. Naturalised Pteridophytes, Gymnosperms, Dicotyledons. Christchurch, New Zealand, Botany Division, 1988.

[3]. Havsteen, B. H. Pharmacol. Therapeut. 2002, 96, 67-202.
http://dx.doi.org/10.1016/S0163-7258(02)00298-X

[4]. Martin, S.; Andriantsitohaina, R. Ann. Cardiol. Angeiol. 2002, 51, 304-315.
http://dx.doi.org/10.1016/S0003-3928(02)00138-5

[5]. Noguchi, C.; Niki, E. Free Radical Bio. Med. 2000, 28, 1538-1546.
http://dx.doi.org/10.1016/S0891-5849(00)00256-2

[6]. Nunez-Selles, A. J. J. Braz. Chem. Soc. 2005, 16, 699-710.
http://dx.doi.org/10.1590/S0103-50532005000500004

[7]. Syed, D. N.; Afaq, F.; Mukhtar, H. Cancer Biol. 2007, 17, 377-385.
http://dx.doi.org/10.1016/j.semcancer.2007.05.004

[8]. Khodakov, G. V.; Yu, A.; Akimov, A. S.; Shashkov, P. K.; Kintia, V. I. Adv. Exp. Med. Biol. 1996, 405, 211-222.
http://dx.doi.org/10.1007/978-1-4613-0413-5_18

[9]. Khodakov, G. V. Chem. Nat. Compd. 2013, 48, 1024-1026.
http://dx.doi.org/10.1007/s10600-013-0455-x

[10]. Braga, P. C.; Sasso, M. D.; Lattuada, N.; Marabini, L.; Calo, R.; Antonacci, R.; Bertelli, A.; Falchi, M.; Verducci, P. J. Med. Plant Res. 2013, 7, 358-365.

[11]. Miri, A.; Rad, J. S.; Alfatemi, S. M. H.; Rad, M. S. Ann. Biol. Res. 2013, 4, 35-41.

[12]. Mosmann, T. J. Immunol. Methods 1983, 65, 55-63.

[13]. Aguiar, R. M.; David, J. P.; David, J. M. Phytochemistry 2005, 66, 2388-2392.
http://dx.doi.org/10.1016/j.phytochem.2005.07.011

[14]. Jin, W.; Tu, P. J. Chromatogr. 2005, 1092, 241-245.
http://dx.doi.org/10.1016/j.chroma.2005.08.009

[15]. Foo, L. Y.; Newman, R.; Waghorn, G.; Mcnabb, W. C.; Ulyatt, M. J. Phytochemistry 1996, 41, 617-624.
http://dx.doi.org/10.1016/0031-9422(95)00602-8

[16]. Chakravarthy, B. K.; Gode, K. D. Planta Med. 1985, 51, 56-59.
http://dx.doi.org/10.1055/s-2007-969393

[17]. Han, X. H.; Hong, S. S.; Hwang, J. S.; Lee, M. K.; Hwang, B. Y.; Ro, J. S. Arch. Pharm. Res. 2007, 30, 13-17.
http://dx.doi.org/10.1007/BF02977772

[18]. Arriaga, A. M. C.; Castro, M. A. B.; Silveira, E. R.; Braz-Filho, R. J. Braz. Chem. Soc. 2000, 11, 187-190.
http://dx.doi.org/10.1590/S0103-50532000000200015

[19]. Zheng, Z.; Cheng, K.; Chao, J.; Wu, J.; Wang, M. Food Chem. 2008, 106, 529-535.
http://dx.doi.org/10.1016/j.foodchem.2007.06.037

[20]. Agrawal, P. K. Carbon-13 NMR of Flavonoids. Elsevier, Amsterdam, 1989.

[21]. Liu, H.; Yan, M.; Jianglin, Z.; Jihua, W.; Ligang, Z.; Mingan, W.; Daoquan, W.; Jianguo, H.; Zhu, Y.; Fuyu, Y. Molecules 2010, 15, 7933-7945.
http://dx.doi.org/10.3390/molecules15117933

[22]. Harborne, J. B. The flavonoids, Advances in research since 1986, Chapman and Hall, London, 1994.

[23]. Vvedenskaya, I. O.; Rosen, T.; Guido, J. E.; Russell, D. J.; Mills, K. A.; Vorsa, N. J. Agricul. Food Chem. 2004, 52, 188-195.
http://dx.doi.org/10.1021/jf034970s

[24]. Ye, G.; Huang, C. Chem. Nat. Compd. 2006, 42, 232-234.
http://dx.doi.org/10.1007/s10600-006-0089-3

[25]. Markham, K. R.; Terani, B.; Stanley, R.; Geiger, H.; Mabry, T. J. Tetrahedron 1999, 50, 329-332.

[26]. El-Toumy, S. A.; El-Sharabasy, F. S.; Ghanem, H. Z.; El-Kady, M. U.; Kassem, A. F. Aust. J. Basic Appl. Sci. 2011, 5, 1362-1370.

[27]. Markham, K. R. Techniques of flavonoid identification. Academic Press, London, 1982.

[28]. Beck, M. A.; Haberlein, H. Phytochemistry 1999, 50, 329-332.
http://dx.doi.org/10.1016/S0031-9422(98)00503-2

[29]. D'Agostino, M.; De Simone, F.; Dini, A.; Ramundo, E.; Zollo, F. Phytochemistry 1990, 29, 353-354.
http://dx.doi.org/10.1016/0031-9422(90)89076-L

[30]. Wang, J.; Gao, H.; Zhao, J.; Wang, Q.; Zhou, L.; Han, J.; Yu, Z.; Yang, F. Molecules 2010, 15, 5998-6007.
http://dx.doi.org/10.3390/molecules15095998

[31]. Kundu, J. K.; Surh, Y. J. Phytochem. Rev. 2009, 8, 333-347.
http://dx.doi.org/10.1007/s11101-009-9132-x

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