2026-05-04T21:02:20Z https://www.eurjchem.com/index.php/eurjchem/oai

oai:ojs.www.eurjchem.com:article/1037 2014-09-30T03:41:50Z eurjchem:ART driver

Micellar high performance liquid chromatographic determination of tinidazole in combination with ciprofloxacin or norfloxacin in bulk, pharmaceutical dosage forms and in spiked human plasma Rizk, Mohamed Toubar, Safaa Shafik El‐Alamin, Maha Mahmoud Abou Azab, Marwa Mohamed Mahmoud Stability Tinidazole Method validation Anti-protozoal drugs Micellar liquid chromatography Optimization and system suitability A micellar liquid chromatographic method was developed for determination of tinidazole in bulk, dosage forms and human plasma using intersil cyano column and a mobile phase of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, 0.3% triethylamine in 0.02 M orthophosphoric acid:water (60:40, v:v) (pH = 4). The U.V. detection was achieved at 311 nm. Various chromatographic parameters were investigated to select the optimum conditions for the separation, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc. The method was linear over the concentration range 40-200 µg/mL with regression coefficient 0.999. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as stability-indicating method for determination of tinidazole under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters. Atlanta Publishing House LLC 2014-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.eurjchem.com/index.php/eurjchem/article/view/1037 10.5155/eurjchem.5.3.439-445.1037 European Journal of Chemistry; Vol. 5 No. 3 (2014): September 2014; 439-445 2153-2257 2153-2249 eng https://www.eurjchem.com/index.php/eurjchem/article/view/1037/pdf_1037