2026-05-09T17:31:45Z https://www.eurjchem.com/index.php/eurjchem/oai

oai:ojs.www.eurjchem.com:article/1596 2017-09-30T11:59:49Z eurjchem:ART driver

Rapid and sensitive determination of selective progesterone modulator ulipristal acetate in human plasma Pappula, Nagaraju Kodali, Balaji Datla, Peda Varma LC/MS Human plasma Ulipristal acetate ISTD trend analysis Oral contraceptives Liquid-liquid extraction Ulipristal acetate is a new synthetic selective progesterone receptor modulator developed mainly as emergency contraceptive (EC) and also used for the treatment of uterine fibroids. A cost effective, sensitive, simple and rapid high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the analysis of ulipristal acetate in human plasma. Following liquid-liquid extraction, the analyte (Ulipristal acetate) and internal standard (Levonorgestrel) were chromatographed using mobile phase in an isocratic elution mode on a reverse phase C18 column. The LC-MS/MS operated in multiple reaction monitoring mode for respective [M+H]+ ions, m/z 476.2/134.1 for analyte and 313.3/245.1 for internal standard. The assay exhibited linear dynamic range of 1-300 ng/mL. The lower limit of quantification was 1 ng/mL with relative standard deviation of 7.0%. The intra-batch and inter-batch results were precise with coefficient variation of 2.7 to 7.0 (%) and accuracy of 94.2-99.8 (%). The validated method was simple, fast and repeatable for bioequivalence, pharmacokinetic and therapeutic monitoring studies. Atlanta Publishing House LLC 2017-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.eurjchem.com/index.php/eurjchem/article/view/1596 10.5155/eurjchem.8.3.258-264.1596 European Journal of Chemistry; Vol. 8 No. 3 (2017): September 2017; 258-264 2153-2257 2153-2249 eng https://www.eurjchem.com/index.php/eurjchem/article/view/1596/pdf_1596