2026-04-22T17:33:58Z https://www.eurjchem.com/index.php/eurjchem/oai

oai:ojs.www.eurjchem.com:article/1658 2018-03-11T13:45:35Z eurjchem:ART driver

New HPLC method for determination of cystatin C biomarker in human blood AlMusaimi, Othman Ibrahem Abu-Nawwas, Abd-Alhakeem Hasan AlShaer, Danah Mahdi Khaleel, Nabeel Yousef Cystatin C Biomarker Kidney failure Chromatography Glomerular filtration rate Determination of cystatin C A rapid and sensitive method for determination of cystatin C (CC) protein in human blood by means of high performance liquid chromatography (HPLC) was developed and validated. Estimated glomerular filtration rate (eGFR) has been calculated for the patients with chronic kidney disease (CKD). Ion pair liquid chromatography technique was utilized to separate CC along with UV detection. Calibration curve with excellent correlation (r2 = 0.99) over the range from 0.75 to 20.50 mg/L of CC was accomplished. Limits of detection and quantify-cation were 0.375 and 0.75 mg/L of CC, respectively. The recoveries were in the range of 93.6-102.3%. Intra-assay and inter-assay variabilities were 8.2 and 6.14%, respectively. Atlanta Publishing House LLC 2017-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.eurjchem.com/index.php/eurjchem/article/view/1658 10.5155/eurjchem.8.4.378-383.1658 European Journal of Chemistry; Vol. 8 No. 4 (2017): December 2017; 378-383 2153-2257 2153-2249 eng https://www.eurjchem.com/index.php/eurjchem/article/view/1658/pdf_1658