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oai:ojs.www.eurjchem.com:article/1710 2018-06-30T04:10:19Z eurjchem:ART driver

A Validated HPTLC Densitometric Method for Quantitative Determination of Zanamivir in Bulk and Pharmaceutical Formulation Al Bratty, Mohammed Saleh, Safaa Fathy Alhazmi, Hassan Ahmad Javed, Sadique Akhtar Ahmed, Adel Mohammed Ahsan, Waquar HPTLC Diskhaler Zanamivir Densitometric Quantitative determination Pharmaceutical formulation The main purpose of the present study was to develop and validate a high performance thin layer chromatographic (HPTLC) method for quantitative determination of an antiviral agent, zanamivir in pure drug and diskhaler powder formulation. Chromatography was performed on aluminum TLC plates pre-coated with silica gel 60F254, employing a mixture of chloroform:methanol:ammonia (9.5:3.2:0.2,v:v:v) as mobile phase. The TLC scanner was operated in the absorbance mode at a wavelength of 230 nm for evaluation of chromatograms. The system has given well resolved peak of zanamivir (Rf = 0.56). The linearity of the method was established in the range of 20-300 ng/spot; correlation coefficient (r) was 0.9995. The low values of limit of detection and limit of quantification (12.4 and 37.5 ng/spot, respectively) have demonstrated the sensitivity of the developed method. The reported method was precise in both intra-day as well as inter-day analysis; % RSD of peak area was found to be less than 2%, and has an accuracy within 100 ± 2%. The developed method has a potential to quantify zanamivir from its diskhaler formulation without any interference from other components. The applicability of the method was demonstrated by excellent recovery of analyte (99.8%) from diskhaler formulation. The current analytical method can be applied for routine analysis of zanamivir in pure form and pharmaceutical formulation in quality control laboratories.  Atlanta Publishing House LLC 2018-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.eurjchem.com/index.php/eurjchem/article/view/1710 10.5155/eurjchem.9.2.115-120.1710 European Journal of Chemistry; Vol. 9 No. 2 (2018): June 2018; 115-120 2153-2257 2153-2249 eng https://www.eurjchem.com/index.php/eurjchem/article/view/1710/pdf_1710 Copyright (c) 2018 Authors