2026-05-08T15:44:07Z https://www.eurjchem.com/index.php/eurjchem/oai

oai:ojs.www.eurjchem.com:article/239 2011-06-30T10:44:15Z eurjchem:ART driver

In vitro antimicrobial activity of triterpenoid saponin from Tephrosia purpurea seeds extract Khan, Noor Afshan Tephrosia purpurea Spectroscopic analysis Triterpenoid saponin Antibacterial activity Antifungal activity Minimum Inhibitory Concentration A novel oleanen type triterpenoid glycoside has been isolated from the butanolic extract of the seeds of Tephrosia purpurea. Its structure was elucidated as 3-O-{β-D-glucopyranosyl-(1→6)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→2)]-β-D-xylopyranosyl}-2,16-dihydroxy-23,29-dihydroxymethylolean-11,13(18)-diene-28-oic acid on the basis of spectral evidences, i.e. FT-IR, 1H NMR, 13C NMR and FAB-MS data. The isolated saponin was tested for its antimicrobial activity. Significant results were obtained by evaluating the antibacterial activity by “Disc diffusion method” and antifungal activity by “Spore dilution method”. Maximum inhibition was recorded in gram positive bacterium- Streptococcus pneumoniae, while complete inhibition on the growth of fungus-Alternaria alternata was observed at a concentration of 200 μg/mL. The potency of the extract was quantitatively assessed by determining the minimum inhibitory concentration values against selected bacteria. The minimum inhibitory concentration values were in agreement with antibacterial results where minimum value was recorded to be 23 μg/mL for Streptococcus pneumoniae. Atlanta Publishing House LLC 2011-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.eurjchem.com/index.php/eurjchem/article/view/239 10.5155/eurjchem.2.2.189-192.239 European Journal of Chemistry; Vol. 2 No. 2 (2011): June 2011; 189-192 2153-2257 2153-2249 eng https://www.eurjchem.com/index.php/eurjchem/article/view/239/PDF