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oai:ojs.www.eurjchem.com:article/667 2012-12-30T22:55:20Z eurjchem:ART driver

Simultaneous determination of miconazole and hydrocortisone or mometasone using reversed phase liquid chromatography El-Bagary, Ramzia Ismail Elkady, Ehab Farouk Tammam, Marwa Hosny Elmaaty, Ayman Abo Determination Miconazole nitrate Mometasone furoate Hydrocortisone acetate Pharmaceutical preparation Reversed phase liquid chromatography Two simple, rapid and precise reversed phase liquid chromatographic methods have been developed and validated for the simultaneous determination of miconazole nitrate in two binary mixtures, with hydrocortisone acetate (Mixture 1) and mometasone furoate (Mixture 2). For the two mixtures, chromatographic separation was carried out on a C18 column. For mixture 1, a mobile phase consisting of 2.22 mM sodium dihydrogen phosphate (Triethylamine 0.2%):acetonitrile (45:55, v:v) at a flow rate of 0.9 mL/min was used at ambient temperature. Quantitative determination of miconazole and hydrocortisone was achieved with UV detection at 215 and 245 nm, respectively. Linearity, accuracy and precision were found to be acceptable over the concentration range of 30-80 μg/mL for miconazole and 4-80 μg/mL for hydrocotisone. For mixture 2, a mobile phase consisting of acetonitrile:water (Triethylamine 0.2%) (70:30, v:v) at a flow rate of 0.9 mL/min was used at ambient temperature. Quantitative determinations of miconazole and mometasone were achieved with UV detection at 215 and 250 nm, respectively. Linearity, accuracy and precision were found to be acceptable over the concentration range of 10-200 μg/mL for miconazole and 2-60 μg/mL for mometasone. The optimized methods were proved to be specific, robust and accurate for the quality control of the cited drugs in pharmaceutical preparations. Atlanta Publishing House LLC 2012-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.eurjchem.com/index.php/eurjchem/article/view/667 10.5155/eurjchem.3.4.421-425.667 European Journal of Chemistry; Vol. 3 No. 4 (2012): December 2012; 421-425 2153-2257 2153-2249 eng https://www.eurjchem.com/index.php/eurjchem/article/view/667/PDF