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oai:ojs.www.eurjchem.com:article/885 2014-03-31T01:48:51Z eurjchem:ART driver

Micellar high performance liquid chromatographic determination of Itraconazole in bulk, pharmaceutical dosage forms and human plasma Rizk, Mohamed Toubar, Safaa Shafik El-Alamin, Maha Mahmoud Abou Azab, Marwa Mohamed Mahmoud Stability Itraconazole Anti-fungal drugs Method validation Micellar liquid chromatography Optimization and system suitability A micellar liquid chromatographic method was developed for determination of Itraconazole in bulk, dosage form and human plasma using µBondapack cyano column and a mobile phase consisting of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, 0.3% triethylamine in 0.02 M orthophosphoric acid (pH = 3.5). The UV detection was achieved at 258 nm. Various chromatographic parameters were studied, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc .The method was linear over the concentration range 16.4-320.0 µg/mL with regression coefficient 0.999 and limit of detection 5.4 µg/mL in bulk. Inter and intra-day results showed %RSD < 1.49%. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as stability-indicating method for determination of Itraconazole under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters. Atlanta Publishing House LLC 2014-03-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.eurjchem.com/index.php/eurjchem/article/view/885 10.5155/eurjchem.5.1.11-17.885 European Journal of Chemistry; Vol. 5 No. 1 (2014): March 2014; 11-17 2153-2257 2153-2249 eng https://www.eurjchem.com/index.php/eurjchem/article/view/885/PDF_885