2026-05-09T13:49:44Z https://www.eurjchem.com/index.php/eurjchem/oai

oai:ojs.www.eurjchem.com:article/964 2014-06-30T08:07:06Z eurjchem:ART driver

Rapid chiral separation and impurity determination of ropivacaine and bupivacaine by Densitometry-HPTLC, using mucopolysaccharide as chiral mobile phase additive Salama, Nahla Nour El-Din Validation 2 6-Dimethylaniline Densitometry-HPTLC Ropivacaine enantiomers Bupivacaine enantiomers Chiral mobile phase additive Enantio separation of the local anesthetic drugs; ropivacaine (Rop), bupivacaine (Bup) and potential organic impurities (2,6-Dimethylaniline, 2,6-DMA) were accomplished on HPTLC using mucopolysaccharide selector (Chondroitin) as chiral mobile phase additive (CMPA). The enantioseparation was achieved in acetonitrile:water:methanol (16:3:1, v:v:v) containing 0.25% chondroitin as chiral mobile phase additive. The influence of separation conditions, including type and concentration of chiral selector, organic modifiers and temperatures on enantioseparation were evaluated. The enantioselective HPTLC method was validated to control the enantiomeric purity of the (S)-enantiomers (S-Rop and S-Bup); the active ingredients contained in drug products. In these conditions, linearity over the concentration range, 1.0-10.0 µg/spot for each (R)-enantiomer and 1.0-8.0 µg/spot for 2,6-DMA main organic impurity were obtained. The detection limits are less than 0.6 µg/spot of chiral and organic impurities. The intra and inter-day assay precision was less than 3.0% (RSD%). Atlanta Publishing House LLC 2014-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.eurjchem.com/index.php/eurjchem/article/view/964 10.5155/eurjchem.5.2.214-218.964 European Journal of Chemistry; Vol. 5 No. 2 (2014): June 2014; 214-218 2153-2257 2153-2249 eng https://www.eurjchem.com/index.php/eurjchem/article/view/964/pdf_964