2024-03-28T08:57:36Z
https://www.eurjchem.com/index.php/eurjchem/oai
oai:ojs.www.eurjchem.com:article/1000
2014-06-30T08:07:06Z
eurjchem:ART
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A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma
Younes, Kareem Mahmoud
El-Kady, Ehab Farouk
Azithromycin
Sildenafil citrate
Internal standard
Mass spectrometry
Liquid-liquid extraction
Liquid chromatography
A simple, rapid and sensitive LC-MS/MS method was developed and validated for the quantification of azithromycin (AZI) in human plasma. Sildenafil citrate was used as an internal standard. The analytes were extracted from human plasma samples by liquid-liquid extraction technique. The reconstituted samples were chromatographed on a reversed ‑ phase column C18, 50 × 2.1 mm, 5 µm from Waters, by using a 80:20 (v:v) mixture of acetonitrile and 0.1% formic acid in water as the mobile phase at a flow rate of 0.5 mL/min. The calibration curve was linear (r2 > 0.99) over the concentration range of 5.0-1500.0 ng/mL. The results of the intra- and inter-day precision and accuracy studies were within the acceptable limits. A run time of 0.5 min for each sample made it possible to analyze more than 400 plasma samples per day.
Atlanta Publishing House LLC
2014-06-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.eurjchem.com/index.php/eurjchem/article/view/1000
10.5155/eurjchem.5.2.241-246.1000
European Journal of Chemistry; Vol. 5 No. 2 (2014): June 2014; 241-246
2153-2257
2153-2249
eng
https://www.eurjchem.com/index.php/eurjchem/article/view/1000/pdf_1000