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2014-09-30T03:41:50Z
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Micellar high performance liquid chromatographic determination of tinidazole in combination with ciprofloxacin or norfloxacin in bulk, pharmaceutical dosage forms and in spiked human plasma
Rizk, Mohamed
Toubar, Safaa Shafik
El‐Alamin, Maha Mahmoud Abou
Azab, Marwa Mohamed Mahmoud
Stability
Tinidazole
Method validation
Anti-protozoal drugs
Micellar liquid chromatography
Optimization and system suitability
A micellar liquid chromatographic method was developed for determination of tinidazole in bulk, dosage forms and human plasma using intersil cyano column and a mobile phase of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, 0.3% triethylamine in 0.02 M orthophosphoric acid:water (60:40, v:v) (pH = 4). The U.V. detection was achieved at 311 nm. Various chromatographic parameters were investigated to select the optimum conditions for the separation, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc. The method was linear over the concentration range 40-200 µg/mL with regression coefficient 0.999. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as stability-indicating method for determination of tinidazole under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters.
Atlanta Publishing House LLC
2014-09-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.eurjchem.com/index.php/eurjchem/article/view/1037
10.5155/eurjchem.5.3.439-445.1037
European Journal of Chemistry; Vol. 5 No. 3 (2014): September 2014; 439-445
2153-2257
2153-2249
eng
https://www.eurjchem.com/index.php/eurjchem/article/view/1037/pdf_1037