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oai:ojs.www.eurjchem.com:article/1283 2015-09-30T05:16:04Z eurjchem:ART driver

Development and validation of three spectrophotometric methods for determination of pyridostigmine bromide in the presence of its alkaline-induced degradation product Abdelaleem, Eglal Abdelhamid Naguib, Ibrahim Ahmed Abdallah, Fatma Fared Ali, Nouruddin Wageih First derivative method Pyridostigmine bromide Spectrophotometric method First derivative ration spectra Mean centering of ratio spectra 3-Hydoxy-N-methylpyridinium bromide Three simple, accurate and validated spectrophotometric methods were developed for the determination of pyridostigmine bromide (PB) in presence of its alkaline-induced degradation product, 3-hydoxy-N-methylpyridinium bromide (3-OH NMP) in powder form and in pharmaceutical formulations. Method A, is first derivative method (1D), which is based on measuring the peak amplitude of the first derivative spectra (1D) of PB at 260 nm. Method B, is first derivative of ratio spectra (1DD) which allows the determination of PB at 267.4 nm using (50 µg/mL) of 3-OH NMP as a suitable devisor. Finally, method C depends on mean centering of ratio spectra (MCR) of PB with different concentrations, which were recorded over 200-400 nm and divided by the spectrum of 20 µg/mL of 3-OH NMP as a devisor. The obtained ratio spectra were mean centered and the concentrations of PB were then determined from the calibration graphs obtained by measuring the amplitudes at 338 nm. The proposed methods were successfully applied for assay of PB both in pure form and in pharmaceutical formulations. The proposed methods were validated in compliance with International Conference on Harmonization (ICH) guidelines. The results obtained by the developed methods were statistically compared to those obtained by the reported HPLC method using F- and student's t-tests showing no significant difference regarding both accuracy and precision. Atlanta Publishing House LLC 2015-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.eurjchem.com/index.php/eurjchem/article/view/1283 10.5155/eurjchem.6.3.350-356.1283 European Journal of Chemistry; Vol. 6 No. 3 (2015): September 2015; 350-356 2153-2257 2153-2249 eng https://www.eurjchem.com/index.php/eurjchem/article/view/1283/pdf_1283