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2018-03-11T13:45:35Z
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New HPLC method for determination of cystatin C biomarker in human blood
AlMusaimi, Othman Ibrahem
Abu-Nawwas, Abd-Alhakeem Hasan
AlShaer, Danah Mahdi
Khaleel, Nabeel Yousef
Cystatin C
Biomarker
Kidney failure
Chromatography
Glomerular filtration rate
Determination of cystatin C
A rapid and sensitive method for determination of cystatin C (CC) protein in human blood by means of high performance liquid chromatography (HPLC) was developed and validated. Estimated glomerular filtration rate (eGFR) has been calculated for the patients with chronic kidney disease (CKD). Ion pair liquid chromatography technique was utilized to separate CC along with UV detection. Calibration curve with excellent correlation (r2 = 0.99) over the range from 0.75 to 20.50 mg/L of CC was accomplished. Limits of detection and quantify-cation were 0.375 and 0.75 mg/L of CC, respectively. The recoveries were in the range of 93.6-102.3%. Intra-assay and inter-assay variabilities were 8.2 and 6.14%, respectively.
Atlanta Publishing House LLC
2017-12-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.eurjchem.com/index.php/eurjchem/article/view/1658
10.5155/eurjchem.8.4.378-383.1658
European Journal of Chemistry; Vol. 8 No. 4 (2017): December 2017; 378-383
2153-2257
2153-2249
eng
https://www.eurjchem.com/index.php/eurjchem/article/view/1658/pdf_1658