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Crystal structure and spectral studies of green fluorescent protein (GFP) chromophore analogue ethyl 2-[(4Z)-(6-hydroxy naphthalen-2-yl) methylene)-2-methyl-5-oxo-4,5-di hydro-1H-imidazol-1-yl] acetate
Puthuvakkal, Anisha
Manoj, Kochunnoonny
Fluorescent Protein Crystal structure Intermolecular Interactions UV absorption Emission Red shift
Synthetically modified green fluorescent protein chromophore derivative was prepared, its crystal structure and spectral properties were studied. Crystal data for C19H18N2O4: triclinic, space group P-1 (no. 2), a = 8.2506(17) Å, b = 11.934(2) Å, c = 17.461(4) Å, α = 102.89(3)°, β = 94.62(3)°, γ = 96.68(3)°, V = 1654.5(6) Å3, Z = 4, T = 173(2) K, μ(MoKα) = 0.096 mm-1, Dcalc = 1.358 g/cm3, 7227 reflections measured (4.722° ≤ 2Θ ≤ 53.996°), 7227 unique (Rint = 0.0453, Rsigma = 0.0662) which were used in all calculations. The final R1 was 0.0561 (I > 2σ(I)) and wR2 was 0.1658 (all data). The single crystal structure showed, the benzylidine moiety adopts Z-conformation in solid state and the molecules were associated by various O−H···O and C−H···O non-covalent interactions. The UV absorption-emission spectral analysis indicated that a significant red shift of emission observed at alkaline pH indicating its utility for live cell imaging applications.
Atlanta Publishing House LLC
2019-06-30
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info:eu-repo/semantics/publishedVersion
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https://www.eurjchem.com/index.php/eurjchem/article/view/1869
10.5155/eurjchem.10.2.175-179.1869
European Journal of Chemistry; Vol. 10 No. 2 (2019): June 2019; 175-179
2153-2257
2153-2249
eng
https://www.eurjchem.com/index.php/eurjchem/article/view/1869/pdf_1869
https://www.eurjchem.com/index.php/eurjchem/article/view/1869/2633
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