2024-03-28T08:08:39Z
https://www.eurjchem.com/index.php/eurjchem/oai
oai:ojs.www.eurjchem.com:article/966
2014-06-30T08:07:06Z
eurjchem:ART
driver
Development and validation of stability indicating spectrophotometric and HPTLC methods for determination of acemetacin
Naguib, Ibrahim Ahmed
Abdelaleem, Eglal Abdelhamid
Zaazaa, Hala Elsayed
Hussein, Essraa Abd ElWahab
HPTLC
Validation
Acemetacin
Degradation
First derivative of ratio spectra
Mean centering of ratio spectra
Three simple and sensitive methods were developed for the determination of acemetacin (ACM) in presence of its degradation product, indomethacin (DEG). Several methods were evaluated and are presented. Method A was based on measuring the peak amplitude of the first derivative of the ratio spectra 1DD at 244 nm. In method B, mean centering of ratio spectra method (MCR) was applied, which depends on measuring the mean centered values of ratio spectra of ACM at 234 nm. Method C provided separation of ACM from its DEG on pre-activated silica gel 60F254 HPTLC plates using hexane:ethyl acetate:glacial acetic acid (6:4:0.3, v:v:v) as developing system followed by scanning at 254 nm. The suggested methods were validated in compliance with the International Conference on Harmonisation (ICH) guidelines and were successfully applied for quantification of ACM in its commercial capsule. The proposed methods were also statistically compared to a reported HPLC method with no significant difference in performance; indicating the ability of the proposed method to be reliable and suitable for routine analysis of drug product.
Atlanta Publishing House LLC
2014-06-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.eurjchem.com/index.php/eurjchem/article/view/966
10.5155/eurjchem.5.2.219-226.966
European Journal of Chemistry; Vol. 5 No. 2 (2014): June 2014; 219-226
2153-2257
2153-2249
eng
https://www.eurjchem.com/index.php/eurjchem/article/view/966/pdf_966