OPEN ACCESS | PEER-REVIEWED | SHORT COMMUNICATION | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS
Validated RP-HPLC and HPTLC methods for simultaneous estimation of febuxostat and diclofenac sodium in pharmaceutical dosage form
Ponnuveetil Gopi Sunitha (1) , Kaliappan Ilango (2,*)
(1) Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai, 600003, Tamil Nadu, India
(2) Department of Pharmaceutical Chemistry, Sri Ramaswamy Memorial College of Pharmacy, Sri Ramaswamy Memorial University, Kattankulathur, 603203, Tamil Nadu, India
(*) Corresponding Author
Received: 05 Apr 2014 | Revised: 01 May 2014 | Accepted: 01 May 2014 | Published: 30 Sep 2014 | Issue Date: September 2014
The present work reports a reverse phase high performance liquid chromatography (RP-HPLC) method and high performance thin layer chromatography (HPTLC) method for the simultaneous determination of febuxostat and diclofenac sodium in pharmaceutical dosage form. HPLC was performed using a Thermo Hypersil C18 column (250 × 4.6 mm i.d., 5 μm particle size) using a mixture of methanol: KH2PO4 (0.02 M) (70:30, v:v) as mobile phase. Ultraviolet detection was carried out at 280 nm. The retention time of febuxostat and diclofenac sodium were found to be 6.725 and 8.892 min, respectively. The HPTLC separation was conducted on Merck HPTLC aluminum sheets of silica gel 60 F254 as stationary phase using toluene:methanol in the ratio of 7:3 (v:v) as the mobile phase. The detection of febuxostat and diclofenac sodium was carried out at 297 nm. The Rf values of febuxostat and diclofenac sodium were found to be 0.48 and 0.60, respectively. Both the methods were validated as per ICH guidelines. The proposed methods were found to be suitable for the quantification of the selected combination of drugs in pharmaceutical dosage form.
Our editors have decided to support scientists to publish their manuscripts in European Journal of Chemistry without any financial constraints.
1- The article processing fee will not be charged from the articles containing the single-crystal structure characterization between July 1, 2022 and August 15, 2022 (Voucher code: SINGLE2022).
2. Young writers will not be charged for the article processing fee between July 1, 2022 and August 15, 2022 (Voucher code: YOUNG2022).
3. The article processing fee will not be charged from the articles containing a part of the PhD thesis between July 1, 2022 and August 15, 2022 (Voucher code: PhD2022).
European Journal of Chemistry
Links for Article
| | | | | | |
| | | | | | |
| | | |
Article MetricsThis Abstract was viewed 1236 times | PDF Article downloaded 525 times
Centaur Pharmaceuticals Ltd., Pune, India
. Fawzi A. El-Yazbi, Omayma A. Amin, Eman I. El-Kimary, Essam F. Khamis, Sameh E. Younis
HPTLC and Spectrophotometric Estimation of Febuxostat and Diclofenac Potassium in Their Combined Tablets
Journal of Chromatographic Science 54(7), 1146, 2016
. Abdel-Maaboud I. Mohamed, Mahmoud A. Omar, Sayed M. Derayea, Mohamed A. Hammad, Abobakr A. Mohamed
Innovative thin-layer chromatographic method combined with fluorescence detection for specific determination of Febuxostat: Application in biological fluids
Talanta 176, 318, 2018
. Mahmoud A. Omar, Abdel-Maaboud I. Mohamed, Sayed M. Derayea, Mohamed A. Hammad, Abobakr A. Mohamed
Specific and highly sensitive spectrofluorimetric method for determination of febuxostat in its tablets and real human plasma. Application to stability studies
RSC Advances 6(77), 73432, 2016
. Fawzy A El-Yazbi, Omayma A Amin, Eman I El-Kimary, Essam F Khamis, Sameh E Younis, Mohammed AW Elkhatib, Ahmed F El-Yazbi
A novel HPLC-DAD method for simultaneous determination of febuxostat and diclofenac in biological samples: pharmacokinetic outcomes
Bioanalysis 11(1), 41, 2019
. Sweetman, S. C., Martindale: The Complete Drug Reference, 37th edition, Pharmaceutical Press, London, 2011.
. O'Neil, M. J. The Merck Index, 14th edition, Merck Research Laboratories, 2011.
. Bagga, P.; Salman, M.; Siddiqui, H. H.; Ansari, M. A.; Mehmood, T.; Singh, K. Int. J. Pharm. Sci. Res. 2011, 2(10), 2655-2659.
. Kumar, R. J.; Kumar, B. R.; Patnaik, V. V.; Kumar, A. Int. Res. J. Pharm. 2012, 3(11), 205-208.
. Nasare, M.; Harshini, S.; Kumar, A. M.; Diwan, V.P. Int. J. Pharm. Chem. Biol. Sci. 2012, 3(1), 137-142.
. Sudhir, M. S.; Venkata, N. R.; Nadendla, R. R. Int. J. Pharm. Tech. Res. 2012, 4(4), 974-984.
. Krishnareddy, Y.; Sundaraganapathy, A.; Thangadurai, S. A.; Hemanth, G.; Jambulingam, M.; Niraimathi, V. J. Pharm. Res. 2012, 5(7), 3900-3908.
. Rajyalakshmi, C.; Benjamin, T.; Babu, C. R. Int. J. Res. Pharm. Biomed. Sci. 2013, 4(1), 138-144.
. Patel, S. D.; Sharma, N.; Patel, C. M.; Patel, N. B.; Shrivatsav, S. P.; Sanyal, M. J. Adv. Pharm. Sci. Tech. 2013, 1(1), 37-41.
. Mandle, D. S.; Burade, K. B. Int. J. Pharm. Biol. Res. 2013, 4(1), 1-10.
. Mollati, S.; Rao, V.; Jagaveera, K. N. Am. J. Pharm. Res. 2013, 3(2), 406-422.
. Wang, H.; Deng, P.; Chen, X.; Guo, L.; Zhong, D. Biomed. Chromatogr. 2013, 27(1), 34-38.
. Prakash, K.; Patro, S. S.; Shanta, K. K.; Rao, M. E. B. Acta Cienc. Indica. 2004, 135, 135-138.
. Ahmed, N. R. Iraqi Nat. J. Chem. 2011, 44, 467-473.
. Mandava, V.; Rao, B.; Reddy, B. C. K.; Rao, T. S.; Prasanthi, V. Rasayan J. Chem. 2009, 2, 488-490.
. Barry, A.; Mahmood, M.; Hamezh, J. M. J. Kerbala Univ. 2009, 7, 310-316.
. Akshaya, A. K.; Joshi, G. K.; Mishra, P. Ind. J. Pharm. Sci. 1992, 54, 44-46.
. Baria, H. S.; Patil, P.; Modi, H.; Patel, P.; Patel, S.; Chandalia, K. Inv. Rap. Pharm. Anal. Qual. Ass. 2013, 3, 627-631.
. Mothalia, C. P.; Prajapati, K. R.; Parmar, R. R.; Shah, V. N.; Shah, D. A. Int. J. Pharm. Sci. 2013, 4(3), 268-278
. Priya, M. H. Int. J. Pharm. Bio. Sci. 2013, 4(3), 134-138.
. ICH Q2 (R1), Validation of Analytical Procedures: Text and Methodology, The International Federation of Pharmaceutical Manufacturers & Associations, Geneva, Switzerland, 2005.
How to cite
The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item
DOI Link: https://doi.org/10.5155/eurjchem.5.3.545-549.1066
| | | | | | | | |
| | | | | | |
Save to Zotero Save to Mendeley
European Journal of Chemistry 2014, 5(3), 545-549 | doi: https://doi.org/10.5155/eurjchem.5.3.545-549.1066 | Get rights and content
- There are currently no refbacks.
© Copyright 2010 - 2022 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2010-2022 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.