Liquid chromatography-electro spray ionization tandem mass spectrometry for simultaneous determination of Moexipril and its active metabolite Moexiprilat in human plasma
Omar Abd Elaziz (1) , Maha Farouk (2) , Shereen Tawakkol (3) , Ahmed Hemdan (4,*) , Mostafa Shehata (5)
(1) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt
(2) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt
(3) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo, 11431, Egypt
(4) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6th of October, 12566, Egypt
(5) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 12316, Egypt
(*) Corresponding Author
Received: 09 May 2014, Accepted: 24 Aug 2014, Published: 31 Dec 2014
A selective, sensitive, and rapid liquid chromatography-electro spray ionization tandem mass spectrometry method has been developed and subsequently validated for the simultaneous determination of Moexipril (MOX), and its active metabolite Moexiprilat (MOXT) in spiked human plasma, using Benazepril (BENZ) as an internal standard (IS). Various modes were tried and the Multiple Reaction Monitoring (MRM) mode was found the most suitable one. The two analytes and Benazepril (IS) were extracted from human plasma by simple protein precipitation using acetonitrile as the precipitating solvent. The stationary phase used was a C18 Sunfire column while water and acetonitrile at 0.1% formic acid (30:70, v:v) was used as a mobile phase. The flow rate used was 0.8 mL/min. Food and Drug Administration guidelines were followed for the method validation. The linearity range was found to be 0.5-100 ng/mL for MOX and 5-200 ng/mL for MOXT and the correlation coefficient was more than 0.9980 for each analyte. Results for accuracy and precision showed satisfactory results. Also the method was compared with reported HPLC method and no significant difference was found.
This Abstract was viewed 319 times | PDF Article downloaded 236 times
. Moffat, A. C.; Osselton, M. D.; Widdop, B. Clarke's Analysis of Drugs and Poisons, Pharmaceutical Press, London, UK, 2005.
. Brogden, R. N.; Wiseman, L. R. Drugs 1998, 55, 845-860.
. Chrysant, S. G.; Chrysant, G. S. J. Clin. Pharmacol. 2004, 44, 827-836.
. Stanisz, B.; Regulska, K.; Ratajczak, T. Acta Pol. Pharm. Drug Res. 2012, 69, 389-395.
. Latha, Y. B.; Sankar, D. G. Int. J. Univ. Pharm. Life Sci. 2011, 1, 156-165.
. Elshanawane, A. A.; Mostafa, S. M.; Elgawish, M. S. Chromatographia 2008, 67, 567-573.
. Pandey, R.; Patil, P. O.; Bari, S. B.; Dhumal, D. M. Asian J. Biochem. Pharm. Res. 2012, 2, 342-347.
. Abd El Kawy, M.; El Gindy, A. E.; Hegazy, M.; Shokry, E. S. J. Appl. Sci. Res. 2010, 6, 918-926.
. Hammes, W.; Hammes, B.; Büchsler, U.; Paar, F.; Bökens, H. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 1995, 670, 81-89.
. Koti, J.; Hada, V.; Petroianu, G.; Hasan, M. Y.; Tekes, K.; Szücs, Z.; Kalasz, H. J. Chromatogr. Sci. 2006, 44, 214-218.
. Karra, V. K.; Mullangi, R.; Pilli, N. R.; Inamadugu, J. K.; Ravi, V. B.; Seshagiri, J. V. Biomed. Chromatogr. 2012, 26, 1552-1558.
. Guidance for Industry, Bioanalytical Method Validation, US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER) Center for Veterinary Medicine (CVM), USA, 2001.
How to cite
DOI Link: https://doi.org/10.5155/eurjchem.5.4.662-667.1090
- There are currently no refbacks.
© Copyright 2019 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2019 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 4614 Lavista road, Tucker, GA, 30084, USA. Registered in USA.