

Liquid chromatography-electro spray ionization tandem mass spectrometry for simultaneous determination of Moexipril and its active metabolite Moexiprilat in human plasma
Omar Abd Elaziz (1)





(1) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt
(2) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt
(3) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo, 11431, Egypt
(4) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6th of October, 12566, Egypt
(5) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 12316, Egypt
(*) Corresponding Author
Received: 09 May 2014 | Revised: 04 Jul 2014 | Accepted: 24 Aug 2014 | Published: 31 Dec 2014 | Issue Date: December 2014
Abstract
A selective, sensitive, and rapid liquid chromatography-electro spray ionization tandem mass spectrometry method has been developed and subsequently validated for the simultaneous determination of Moexipril (MOX), and its active metabolite Moexiprilat (MOXT) in spiked human plasma, using Benazepril (BENZ) as an internal standard (IS). Various modes were tried and the Multiple Reaction Monitoring (MRM) mode was found the most suitable one. The two analytes and Benazepril (IS) were extracted from human plasma by simple protein precipitation using acetonitrile as the precipitating solvent. The stationary phase used was a C18 Sunfire column while water and acetonitrile at 0.1% formic acid (30:70, v:v) was used as a mobile phase. The flow rate used was 0.8 mL/min. Food and Drug Administration guidelines were followed for the method validation. The linearity range was found to be 0.5-100 ng/mL for MOX and 5-200 ng/mL for MOXT and the correlation coefficient was more than 0.9980 for each analyte. Results for accuracy and precision showed satisfactory results. Also the method was compared with reported HPLC method and no significant difference was found.
Announcements
Our editors have decided to support scientists to publish their manuscripts in European Journal of Chemistry without any financial constraints.
1- The article processing fee will not be charged from the articles containing the single-crystal structure characterization or a DFT study between September 15, 2023 and October 31, 2023 (Voucher code: FALL2023).
2. A 50% discount will be applied to the article processing fee for submissions made between September 15, 2023 and October 31, 2023 by authors who have at least one publication in the European Journal of Chemistry (Voucher code: AUTHOR-3-2023).
3. Young writers will not be charged for the article processing fee between September 15, 2023 and October 31, 2023 (Voucher code: YOUNG2023).
Editor-in-Chief
European Journal of Chemistry
Keywords
Full Text:
PDF

DOI: 10.5155/eurjchem.5.4.662-667.1090
Links for Article
| | | | | | |
| | | | | | |
| | | |
Related Articles
Article Metrics


Funding information
Genuine Research Centre (GRC), Cairo, Egypt
References
[1]. Moffat, A. C.; Osselton, M. D.; Widdop, B. Clarke's Analysis of Drugs and Poisons, Pharmaceutical Press, London, UK, 2005.
[2]. Brogden, R. N.; Wiseman, L. R. Drugs 1998, 55, 845-860.
http://dx.doi.org/10.2165/00003495-199855060-00018
[3]. Chrysant, S. G.; Chrysant, G. S. J. Clin. Pharmacol. 2004, 44, 827-836.
http://dx.doi.org/10.1177/0091270004267194
[4]. Stanisz, B.; Regulska, K.; Ratajczak, T. Acta Pol. Pharm. Drug Res. 2012, 69, 389-395.
[5]. Latha, Y. B.; Sankar, D. G. Int. J. Univ. Pharm. Life Sci. 2011, 1, 156-165.
[6]. Elshanawane, A. A.; Mostafa, S. M.; Elgawish, M. S. Chromatographia 2008, 67, 567-573.
http://dx.doi.org/10.1365/s10337-008-0544-3
[7]. Pandey, R.; Patil, P. O.; Bari, S. B.; Dhumal, D. M. Asian J. Biochem. Pharm. Res. 2012, 2, 342-347.
[8]. Abd El Kawy, M.; El Gindy, A. E.; Hegazy, M.; Shokry, E. S. J. Appl. Sci. Res. 2010, 6, 918-926.
[9]. Hammes, W.; Hammes, B.; Büchsler, U.; Paar, F.; Bökens, H. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 1995, 670, 81-89.
http://dx.doi.org/10.1016/0378-4347(95)00135-6
[10]. Koti, J.; Hada, V.; Petroianu, G.; Hasan, M. Y.; Tekes, K.; Szücs, Z.; Kalasz, H. J. Chromatogr. Sci. 2006, 44, 214-218.
http://dx.doi.org/10.1093/chromsci/44.4.214
[11]. Karra, V. K.; Mullangi, R.; Pilli, N. R.; Inamadugu, J. K.; Ravi, V. B.; Seshagiri, J. V. Biomed. Chromatogr. 2012, 26, 1552-1558.
http://dx.doi.org/10.1002/bmc.2731
[12]. Guidance for Industry, Bioanalytical Method Validation, US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER) Center for Veterinary Medicine (CVM), USA, 2001.
Supporting information
The Supplementary Material for this article can be found online at: Supplementary files
How to cite
The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item
DOI Link: https://doi.org/10.5155/eurjchem.5.4.662-667.1090

















European Journal of Chemistry 2014, 5(4), 662-667 | doi: https://doi.org/10.5155/eurjchem.5.4.662-667.1090 | Get rights and content
Refbacks
- There are currently no refbacks.
Copyright (c)
© Copyright 2010 - 2023 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2010-2023 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.