European Journal of Chemistry 2014, 5(4), 662-667. doi:10.5155/eurjchem.5.4.662-667.1090

Liquid chromatography-electro spray ionization tandem mass spectrometry for simultaneous determination of Moexipril and its active metabolite Moexiprilat in human plasma


Omar Abd Elaziz (1) , Maha Farouk (2) , Shereen Tawakkol (3) , Ahmed Hemdan (4,*) , Mostafa Shehata (5)

(1) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt
(2) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt
(3) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo, 11431, Egypt
(4) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6th of October, 12566, Egypt
(5) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 12316, Egypt
(*) Corresponding Author

Received: 09 May 2014, Accepted: 24 Aug 2014, Published: 31 Dec 2014

Abstract


A selective, sensitive, and rapid liquid chromatography-electro spray ionization tandem mass spectrometry method has been developed and subsequently validated for the simultaneous determination of Moexipril (MOX), and its active metabolite Moexiprilat (MOXT) in spiked human plasma, using Benazepril (BENZ) as an internal standard (IS). Various modes were tried and the Multiple Reaction Monitoring (MRM) mode was found the most suitable one. The two analytes and Benazepril (IS) were extracted from human plasma by simple protein precipitation using acetonitrile as the precipitating solvent. The stationary phase used was a C18 Sunfire column while water and acetonitrile at 0.1% formic acid (30:70, v:v) was used as a mobile phase. The flow rate used was 0.8 mL/min. Food and Drug Administration guidelines were followed for the method validation. The linearity range was found to be 0.5-100 ng/mL for MOX and 5-200 ng/mL for MOXT and the correlation coefficient was more than 0.9980 for each analyte. Results for accuracy and precision showed satisfactory results. Also the method was compared with reported HPLC method and no significant difference was found.


Keywords


Plasma; Moexipril; LC-MS/MS; Benazepril; Moexiprilat; Simple protein precipitation

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DOI: 10.5155/eurjchem.5.4.662-667.1090

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How to cite


Elaziz, O.; Farouk, M.; Tawakkol, S.; Hemdan, A.; Shehata, M. Eur. J. Chem. 2014, 5(4), 662-667. doi:10.5155/eurjchem.5.4.662-667.1090
Elaziz, O.; Farouk, M.; Tawakkol, S.; Hemdan, A.; Shehata, M. Liquid chromatography-electro spray ionization tandem mass spectrometry for simultaneous determination of Moexipril and its active metabolite Moexiprilat in human plasma. Eur. J. Chem. 2014, 5(4), 662-667. doi:10.5155/eurjchem.5.4.662-667.1090
Elaziz, O., Farouk, M., Tawakkol, S., Hemdan, A., & Shehata, M. (2014). Liquid chromatography-electro spray ionization tandem mass spectrometry for simultaneous determination of Moexipril and its active metabolite Moexiprilat in human plasma. European Journal of Chemistry, 5(4), 662-667. doi:10.5155/eurjchem.5.4.662-667.1090
Elaziz, Omar, Maha Farouk, Shereen Tawakkol, Ahmed Hemdan, & Mostafa Shehata. "Liquid chromatography-electro spray ionization tandem mass spectrometry for simultaneous determination of Moexipril and its active metabolite Moexiprilat in human plasma." European Journal of Chemistry [Online], 5.4 (2014): 662-667. Web. 19 Sep. 2019
Elaziz, Omar, Farouk, Maha, Tawakkol, Shereen, Hemdan, Ahmed, AND Shehata, Mostafa. "Liquid chromatography-electro spray ionization tandem mass spectrometry for simultaneous determination of Moexipril and its active metabolite Moexiprilat in human plasma" European Journal of Chemistry [Online], Volume 5 Number 4 (31 December 2014)

DOI Link: https://doi.org/10.5155/eurjchem.5.4.662-667.1090

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