European Journal of Chemistry

Determination of metformin hydrochloride in human plasma by UPLC/MS/MS: Application in bioequivalence study

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Published: Jun 30, 2015
DOI: https://doi.org/10.5155/eurjchem.6.2.178-182.1234
Keywords:
Plasma, Validation, UPLC/MS/MS, Bioequivalence, Chlorpheneramine, Metformin hydrochloride
Section
Research Article
Issue
Vol. 6 No. 2 (2015): June 2015
Dates
Received 2014-12-24
Accepted 2015-02-14
Published 2015-06-30


Main Article Content

Samah Abd Elsabour Mohammed
National Organization of Drug Control and Research, Giza, 11843, Egypt
Mohammed El-Dardiri
Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt
Mona Ahmed Elhabak
Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt
Khaled Ibrahim Abu Zaid
Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt

Abstract

Metformin hydrochloride was quantified in human plasma by Ultra Performance Liquid Chromatography with Tandem Mass spectrometric determination (UPLC-MS/MS). The mobile phase used was (Water + 0.1% Formic acid) and (Acetonitrile + 0.1% Formic acid) in the ratio of 80:20 (v:v). An Acquity UPLC HSS T3 1.8 um 2.1×50 mm column was used. The detection was performed on a mass spectrometer (ESI+) using chlorpheniramine as an internal standard. The method was fully validated and it was applied to bioequivalence study of metformin hydrochloride in two brands of the drug with relative bioequivalence of 94.7%.


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How to Cite
(1)
Mohammed, S. A. E.; El-Dardiri, M.; Elhabak, M. A.; Abu Zaid, K. I. Determination of Metformin Hydrochloride in Human Plasma by UPLC MS MS: Application in Bioequivalence Study. Eur. J. Chem. 2015, 6, 178-182.

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Supporting Agencies

MEPACO Pharmaceutical Company (Arab Company for Pharmaceutical and Medicinal Plant), Elsharkya, Egypt
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