OPEN ACCESS | PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS
Determination of metformin hydrochloride in human plasma by UPLC/MS/MS: Application in bioequivalence study
Samah Abd Elsabour Mohammed (1,*) , Mohammed El-Dardiri (2) , Mona Ahmed Elhabak (3) , Khaled Ibrahim Abu Zaid (4)
(1) National Organization of Drug Control and Research, Giza, 11843, Egypt
(2) Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt
(3) Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt
(4) Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt
(*) Corresponding Author
Received: 24 Dec 2014 | Revised: 14 Feb 2015 | Accepted: 14 Feb 2015 | Published: 30 Jun 2015 | Issue Date: June 2015
Metformin hydrochloride was quantified in human plasma by Ultra Performance Liquid Chromatography with Tandem Mass spectrometric determination (UPLC-MS/MS). The mobile phase used was (Water + 0.1% Formic acid) and (Acetonitrile + 0.1% Formic acid) in the ratio of 80:20 (v:v). An Acquity UPLC HSS T3 1.8 um 2.1×50 mm column was used. The detection was performed on a mass spectrometer (ESI+) using chlorpheniramine as an internal standard. The method was fully validated and it was applied to bioequivalence study of metformin hydrochloride in two brands of the drug with relative bioequivalence of 94.7%.
Links for Article
| | | | |
| | | | | | |
Article MetricsThis Abstract was viewed 515 times | PDF Article downloaded 287 times
MEPACO Pharmaceutical Company (Arab Company for Pharmaceutical and Medicinal Plant), Elsharkya, Egypt
. O'Neil, M. J.; Heckelman P. E.; Koch C. B.; Roman K. J. The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals, 4th Edition, Merck & Co., Inc., Whitehouse Station, NJ, 2012.
. Goodarzi, M. O.; Bryer-Ash, M. Diabetes Obes. Metab. 2005, 7(6), 654-665.
. Holt, R. I. G.; Cockram, C.; Flyvbjerg, A.; Goldstein, B. J. Text book for diabetes, 4th edition, John Wiley & Sons, 2009.
. Liu, A.; Coleman, S. P. J. Chromatogr. B 2009, 877(29), 3695-3700.
. Chen, L.; Zhou, Z.; Shen, M.; Ma, A. J. Chromatogr. Sci. 2011, 49, 94-100.
. Chen, X.; Gu, Q.; Qiu, F.; Dafa, Z. D. J. Chromatogr. B 2004, 802(2), 377-381.
. Marlice, A.; Marques, S.; Soares, A.; Pinto, O. W.; Werneck, B. P. T. J. Chromatogr. B 2007, 852, 308-316.
. Ding, C. G.; Zhou, Z.; Ge, Q.; Zhi, X.; Ma, L. L. Biomed. Chromatogr. 2007, 21(2), 132-138.
. Ranetti, M. C.; Ionescu, M.; Hinescu, L.; Ionica, E.; Anuta, V.; Ranetti, A. E.; Stecoza, C. E.; Mircioiu, C. Farmacia 2009, 57(6), 728-735.
. Bonde, S. L.; Bhadane, R. P.; Gaikwad, A.; Katale, D.; Gavali, S.; Narendiran, A. S. A. Int. J. Pharm. Sci. 2013, 3, 463-470.
. Amini, H; Ahmadiani, A.; Gazerani, P. J. Chromatogr. B 2005, 824(1-2), 319-322.
. Jian, W.; Ying, W. G.; Jing-kai, G. U.; Yan, W. Chem. Res. Chinese. 2005, 21(2), 246-250.
. Zhang, W.; Han, F.; Zhao, H.; Lin, Z. J.; Huang, Q. M.; Weng, N. Biomed. Chromatogr. 2011, 26(10), 1163-1169.
. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research. Guidance for Industry-Bioanalytical Method validation, MD, 2001.
How to cite
DOI Link: https://doi.org/10.5155/eurjchem.6.2.178-182.1234
| | | | |
| | | | | |
Save to Zotero Save to Mendeley
European Journal of Chemistry 2015, 6(2), 178-182 | doi: https://doi.org/10.5155/eurjchem.6.2.178-182.1234 | Get rights and content
- There are currently no refbacks.
© Copyright 2019 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2019 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 4614 Lavista road, Tucker, GA, 30084, USA. Registered in USA.