European Journal of Chemistry

Determination of metformin hydrochloride in human plasma by UPLC/MS/MS: Application in bioequivalence study

Article Sidebar

PDF
Published: Jun 30, 2015
DOI: https://doi.org/10.5155/eurjchem.6.2.178-182.1234
Keywords:
Plasma, Validation, UPLC/MS/MS, Bioequivalence, Chlorpheneramine, Metformin hydrochloride
Section
Research Article
Issue
Vol. 6 No. 2 (2015): June 2015
Dates
Received 2014-12-24
Accepted 2015-02-14
Published 2015-06-30


Main Article Content

Samah Abd Elsabour Mohammed
National Organization of Drug Control and Research, Giza, 11843, Egypt
Mohammed El-Dardiri
Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt
Mona Ahmed Elhabak
Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt
Khaled Ibrahim Abu Zaid
Bioequivalence Center, Modern Science and Arts University, 6th October, 11787, Egypt

Abstract

Metformin hydrochloride was quantified in human plasma by Ultra Performance Liquid Chromatography with Tandem Mass spectrometric determination (UPLC-MS/MS). The mobile phase used was (Water + 0.1% Formic acid) and (Acetonitrile + 0.1% Formic acid) in the ratio of 80:20 (v:v). An Acquity UPLC HSS T3 1.8 um 2.1×50 mm column was used. The detection was performed on a mass spectrometer (ESI+) using chlorpheniramine as an internal standard. The method was fully validated and it was applied to bioequivalence study of metformin hydrochloride in two brands of the drug with relative bioequivalence of 94.7%.


icon graph This Abstract was viewed 1621 times | icon graph Article PDF downloaded 963 times

How to Cite
(1)
Mohammed, S. A. E.; El-Dardiri, M.; Elhabak, M. A.; Abu Zaid, K. I. Determination of Metformin Hydrochloride in Human Plasma by UPLC MS MS: Application in Bioequivalence Study. Eur. J. Chem. 2015, 6, 178-182.

Article Details

Share
Links for Article


Crossref - Scopus - Google - European PMC
Crossref
Scopus
Google Scholar
Europe PMC
References

[1]. O'Neil, M. J.; Heckelman P. E.; Koch C. B.; Roman K. J. The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals, 4th Edition, Merck & Co., Inc., Whitehouse Station, NJ, 2012.

[2]. Goodarzi, M. O.; Bryer-Ash, M. Diabetes Obes. Metab. 2005, 7(6), 654-665.
http://dx.doi.org/10.1111/j.1463-1326.2004.00448.x

[3]. Holt, R. I. G.; Cockram, C.; Flyvbjerg, A.; Goldstein, B. J. Text book for diabetes, 4th edition, John Wiley & Sons, 2009.

[4]. Liu, A.; Coleman, S. P. J. Chromatogr. B 2009, 877(29), 3695-3700.
http://dx.doi.org/10.1016/j.jchromb.2009.09.020

[5]. Chen, L.; Zhou, Z.; Shen, M.; Ma, A. J. Chromatogr. Sci. 2011, 49, 94-100.
http://dx.doi.org/10.1093/chrsci/49.2.94

[6]. Chen, X.; Gu, Q.; Qiu, F.; Dafa, Z. D. J. Chromatogr. B 2004, 802(2), 377-381.
http://dx.doi.org/10.1016/j.jchromb.2003.12.017

[7]. Marlice, A.; Marques, S.; Soares, A.; Pinto, O. W.; Werneck, B. P. T. J. Chromatogr. B 2007, 852, 308-316.
http://dx.doi.org/10.1016/j.jchromb.2007.01.030

[8]. Ding, C. G.; Zhou, Z.; Ge, Q.; Zhi, X.; Ma, L. L. Biomed. Chromatogr. 2007, 21(2), 132-138.
http://dx.doi.org/10.1002/bmc.723

[9]. Ranetti, M. C.; Ionescu, M.; Hinescu, L.; Ionica, E.; Anuta, V.; Ranetti, A. E.; Stecoza, C. E.; Mircioiu, C. Farmacia 2009, 57(6), 728-735.

[10]. Bonde, S. L.; Bhadane, R. P.; Gaikwad, A.; Katale, D.; Gavali, S.; Narendiran, A. S. A. Int. J. Pharm. Sci. 2013, 3, 463-470.

[11]. Amini, H; Ahmadiani, A.; Gazerani, P. J. Chromatogr. B 2005, 824(1-2), 319-322.
http://dx.doi.org/10.1016/j.jchromb.2005.07.009

[12]. Jian, W.; Ying, W. G.; Jing-kai, G. U.; Yan, W. Chem. Res. Chinese. 2005, 21(2), 246-250.

[13]. Zhang, W.; Han, F.; Zhao, H.; Lin, Z. J.; Huang, Q. M.; Weng, N. Biomed. Chromatogr. 2011, 26(10), 1163-1169.
http://dx.doi.org/10.1002/bmc.2673

[14]. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research. Guidance for Industry-Bioanalytical Method validation, MD, 2001.

Supporting Agencies

MEPACO Pharmaceutical Company (Arab Company for Pharmaceutical and Medicinal Plant), Elsharkya, Egypt
Most read articles by the same author(s)
TrendMD

Dimensions - Altmetric - scite_ - PlumX

Downloads and views

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...
License Terms

License Terms

by-nc

Copyright © 2025 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at https://www.eurjchem.com/index.php/eurjchem/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (https://www.eurjchem.com/index.php/eurjchem/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).