

Spectral resolution and simultaneous determination of oxymetazoline hydrochloride and sodium cromoglycate by derivative and ratio-based spectrophotometric methods
Maha Abdel Monem Hegazy (1)




(1) Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 11511, Egypt
(2) Bioanalysis Research Group, Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 11511, Egypt
(3) Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 11511, Egypt
(4) Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 11511, Egypt
(*) Corresponding Author
Received: 04 Jun 2015 | Accepted: 05 Jul 2015 | Published: 30 Sep 2015 | Issue Date: September 2015
Abstract
Sodium cromoglycate (SCG) and oxymetazoline hydrochloride (OXMT) are administered in combination for effective treatment of nasal congestion and allergy. In this work, SCG was determined using direct spectrophotometry by measuring its zero order absorption spectra at its λmax 320.6 nm where OXMT showed zero absorbance. On the other hand, four simple, sensitive and precise spectrophotometric methods were developed and validated for the determination of OXMT in the presence of SCG in their laboratory prepared mixtures and pharmaceutical formulation, without preliminary separation; Method A: first derivative spectrophotometric method [1D], Method B: first derivative of ratio spectra method [1DD], Method C: ratio difference spectrophotometric method [RDSM] and Method D: ratio subtraction method [RSM]. Ratio manipulating methods (Method B, C and D) were done using divisor of 10.00 µg/mL SCG. Linear correlation was obtained in range 4-22 µg/mL for OXMT by methods A, B and D and 6-22 µg/mL for method C. All methods were validated in compliance with the International Conference on Harmonization (ICH) guidelines and satisfactory results were obtained. No significant difference was noted between the developed methods and the official one with respect to accuracy and precision.
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DOI: 10.5155/eurjchem.6.3.319-324.1278
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[2]. Maha A. Hegazy, May H. Abdelwahab, Hassan A. M. Hendawy, Soheir A. Weshahy, Samah S. Abbas
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References
[1]. Spina, D. Drugs for the Treatment of Respiratory Diseases, Cambridge University Press, 2003.
http://dx.doi.org/10.1017/CBO9780511545696
[2]. Widdicombe, J. Allergy 1997, 52(40), 7-11.
[3]. Abdel-Aziz, O.; El-Kosasy, A.; Magdy, N.; El Zahar, N. Spectrochim. Acta A 2014, 131, 59-66.
http://dx.doi.org/10.1016/j.saa.2014.04.047
[4]. Ochoa, E. A.; Zaton, A. M. Spectrochim. ActaA1998, 54, 983-988.
http://dx.doi.org/10.1016/S1386-1425(98)00056-0
[5]. Helle, A.; Hirsjarvi, S.; Peltonen, L.; Hirvonen, J.; Wiedmer, S. K. J. Chromtogr. A 2008, 1178, 248-255.
http://dx.doi.org/10.1016/j.chroma.2007.11.041
[6]. Pereira, F.; Fogg, A.; Zanoni, M. Talanta 2003, 60, 1023-1032.
http://dx.doi.org/10.1016/S0039-9140(03)00181-4
[7]. Moreira, J. C.; Foster, S. E.; Rodrigues, J. A.; Fogg, A. G. Analyst 1992, 117, 989-991.
http://dx.doi.org/10.1039/an9921700989
[8]. Liu, X. Y.; Qu, T. T.; Wang, B. J.; Wei, C. M.; Yuan, G. Y.; Zhang, R.; Guo, R. C. Biomed. Chromatogr. 2008, 22, 1021-1027.
http://dx.doi.org/10.1002/bmc.1022
[9]. Barnes, M.; Mansfield, R.; Thatcher, S. J. Liq. Chromtogr. R. T. 2008, 25, 1721-1745.
http://dx.doi.org/10.1081/JLC-120005869
[10]. Ozoux, M.; Girault, J.; Malgouyat, J.; Pasquier, O. J. Chromtogr. B 2001, 765, 179-185.
http://dx.doi.org/10.1016/S0378-4347(01)00420-0
[11]. Segall, A.; Vitale, F.; Ricci, R.; Giancaspro, G.; Pizzorno, M. T. Drug Dev. Ind. Pharm. 1997, 23, 839-842.
http://dx.doi.org/10.3109/03639049709150555
[12]. Mawatari, K. I.; Mashiko, S.; Sate, Y.; Usui, Y.; Iinuma, F.; Watanabe, M. Analyst 1997, 122, 715-717.
http://dx.doi.org/10.1039/a608152e
[13]. Ng, L. L. J. Aoac. Int. 1993, 77, 1689-1694.
[14]. Gardner, J. J. J. Chromtogr. B 1984, 305, 228-232.
http://dx.doi.org/10.1016/S0378-4347(00)83335-6
[15]. Wang, N. N.; Shao, Y. Q.; Tang, Y. H.; Yin, H. P.; Wu, X. Z. Luminescence 2009, 24, 178-182.
http://dx.doi.org/10.1002/bio.1092
[16]. Zamora, L. L.; Mestre, Y. F.; Duart, M.; Fos, G. A.; Domenech, R. G.; Alvarez, J. G.; Calatayud, J. M. Anal. Chem. 2001, 73, 4301-4306.
http://dx.doi.org/10.1021/ac010133i
[17]. Sankar, D. G.; Sastry, C. S. P.; Reddy, M. N.; Aruna, M. Indian Drugs 1989, 26, 348-351.
[18]. Sankar, D. G.; Sastry, C. S. P.; Reddy, M. N.; Prasad, S. N. R. Indian J. Pharm. Sci. 1987, 49, 69-71.
[19]. Anjaneyulu, Y.; Sekhar, K. C.; Anjaneyulu, V.; Sarma, R. Indian Drugs 1985, 22, 655-657.
[20]. Dixit, R. K.; Misra, S. K.; Awasthi, B. B. Indian Drugs 1984, 22, 31-33.
[21]. Shingbal, D. M. Naik. East. Pharm. 1983, 26, 201-202.
[22]. Kamalapurkar, O. S.; Priolkar, S. R. S. Indian Drugs 1983, 20, 164-166.
[23]. Shingbal, D.; Sawant, K. Indian Drugs 1982, 20, 106-107.
[24]. Chen, Q.; Li, P.; Yang, H.; Li, B.; Zhu, J.; Peng, L. Anal. Bioanal. Chem. 2010, 398, 937-942.
http://dx.doi.org/10.1007/s00216-010-3973-4
[25]. Agusti, M. G.; Pons, L. M.; Coque, M. A. C. G. A.; Romero, J. E. Talanta 2001, 54, 621-630.
http://dx.doi.org/10.1016/S0039-9140(01)00315-0
[26]. Fragkaki, A.; Koupparis, M.; Georgakopoulos, C. Anal. Chim. Acta 2004, 512, 165-171.
http://dx.doi.org/10.1016/j.aca.2004.02.019
[27]. Massaccesi, M. Pharm. Acta Helv. 1986, 62, 302-305.
[28]. Khan, G. A.; Lindberg, R.; Grabic, R.; Fick, J. J. Pharmaceut. Biomed. 2012, 66, 24-32.
http://dx.doi.org/10.1016/j.jpba.2012.02.011
[29]. Vucicevic, K.; Popovic, G.; Nikolic, K.; Vovk, I.; Agbaba, D. J. Liq. Chromtogr. R. T. 2009, 32, 656-667.
http://dx.doi.org/10.1080/10826070802711113
[30]. Golubitskii, G.; Basova, E.; Ivanov, V. J. Anal. Chem. 2008, 63, 875-880.
http://dx.doi.org/10.1134/S1061934808090141
[31]. Sudsakorn, S.; Kaplan, L.; Williams, D. A. J. Pharmaceut. Biomed. 2006, 40, 1273-1280.
http://dx.doi.org/10.1016/j.jpba.2005.09.018
[32]. Detroyer, A.; Schoonjans, V.; Questier, F.; Vander Heyden, Y.; Borosy, A.; Guo, Q.; Massart, D. J. Chromtogr. A 2000, 897, 23-36.
http://dx.doi.org/10.1016/S0021-9673(00)00803-7
[33]. Hayes, F. J.; Baker, T. R.; Dobson, R. L.; Tsueda, M. S. J. Chromtogr. A 1995, 692, 73-81.
http://dx.doi.org/10.1016/0021-9673(94)00630-R
[34]. Orsi, D. D.; Gagliardi, L.; Cavazzutti, G.; Mediati, M.; Tonelli, D. J. Liq. Chromtogr. R. T. 1995, 18, 3233-3242.
http://dx.doi.org/10.1080/10826079508010447
[35]. Hoffmann, T.; Thompson, R.; Seifert, J. Drug Dev. Ind. Pharm. 1989, 15, 743-757.
http://dx.doi.org/10.3109/03639048909058529
[36]. United States Pharmacopoeia and National Formulary (USP 34-NF 29). United States Pharmacopoeial convention: Rockville, 2011.
[37]. El-Bardicy, M. G.; Lotfy, H. M.; El-Sayed, M. A.; El-Tarras, M. F. J. Aoac. Int. 2008, 91, 299-310.
[38]. Lotfy, H. M.; Abdel-Monemhagazy, M. Spectrochim. Acta A 2012, 96, 259-270.
http://dx.doi.org/10.1016/j.saa.2012.04.095
[39]. Lotfy, H. M.; Saleh, S. S.; Hassan, N. Y.; Elgizawy, S. M. Am. J. Anal. Chem. 2012, 3, 761-769.
http://dx.doi.org/10.4236/ajac.2012.311101
[40]. Abdel-Aleem, E. A.; Hegazy, M. A.; Sayed, N. W.; Abdelkawy, M.; Abdelfatah, R. M. Spectrochim. Acta A 2015, 136, 707-713.
http://dx.doi.org/10.1016/j.saa.2014.09.085
[41]. Ramadan, N. K.; El-Ragehy, N. A.; Ragab, M. T.; El-Zeany, B. A. Spectrochim. Acta A 2015, 137, 463-470.
http://dx.doi.org/10.1016/j.saa.2014.09.003
[42]. ICH, Q2 (R1) Validation of Analytical Procedures, in: Proceeding of the International Conference on Harmonization. Geneva, 2005.
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