European Journal of Chemistry 2010, 1(4), 307-311 | doi: | Get rights and content

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Thermodynamic investigation of a new water-soluble porphyrin with calf thymus DNA

Hamid Dezhampanah (1,*) , Abdol-Khalegh Bordbar (2) , Shahram Tangestaninejad (3)

(1) Department of Chemistry, Faculty of Science, University of Guilan, Rasht, IR-0098, Iran
(2) Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, Isfahan, IR-81746-73441, Iran
(3) Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, Isfahan, IR-81746-73441, Iran
(*) Corresponding Author

Received: 07 Jun 2010 | Accepted: 21 Sep 2010 | Published: 22 Dec 2010 | Issue Date: December 2010


The equilibrium binding of a new water-soluble tetra-cationic porphyrin, 5-(1-(4-carboxybutyl)‎pyridinium-4-yl)10,15,20-tris(1-methylpyridinium-4-yl)‎porphyrin (5-CBPyP) with calf thymus DNA in comparison with meso-tetrakis(4-N-methyl‎pyridinium)porphyrin (TMPyP) has been studied in 7.5 mM phosphate buffer, pH=7.2; and at various temperatures by UV-Vis absorption, fluorescence spectroscopies and viscosity measurement. The thermodynamic parameters were calculated by van't Hoff equation at various temperatures. The values of -137.13±1.22 kJ/mol and -337.21±4.75 J/mol.K for 5-CBPyP and -159.12±1.22 kJ/mol and -406.11±4.45 J/mol.K for TMPyP, were estimated for enthalpy and entropy changes of interaction, respectively. The data indicate that the process is exothermic and enthalpy driven suggesting that electrostatic forces play a considerable role in the interaction process. The results of spectroscopic techniques and viscosity measurement represent the intercalation mode of binding for both porphyrins and higher binding affinity of TMPyP respect to 5-CBPyP.



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European Journal of Chemistry


Porphyrin; Thermodynamics of binding; Optical absorption; Fluorescence

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DOI: 10.5155/eurjchem.1.4.307-311.155

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How to cite

Dezhampanah, H.; Bordbar, A.; Tangestaninejad, S. Eur. J. Chem. 2010, 1(4), 307-311. doi:10.5155/eurjchem.1.4.307-311.155
Dezhampanah, H.; Bordbar, A.; Tangestaninejad, S. Thermodynamic investigation of a new water-soluble porphyrin with calf thymus DNA. Eur. J. Chem. 2010, 1(4), 307-311. doi:10.5155/eurjchem.1.4.307-311.155
Dezhampanah, H., Bordbar, A., & Tangestaninejad, S. (2010). Thermodynamic investigation of a new water-soluble porphyrin with calf thymus DNA. European Journal of Chemistry, 1(4), 307-311. doi:10.5155/eurjchem.1.4.307-311.155
Dezhampanah, Hamid, Abdol-Khalegh Bordbar, & Shahram Tangestaninejad. "Thermodynamic investigation of a new water-soluble porphyrin with calf thymus DNA." European Journal of Chemistry [Online], 1.4 (2010): 307-311. Web. 26 Nov. 2022
Dezhampanah, Hamid, Bordbar, Abdol-Khalegh, AND Tangestaninejad, Shahram. "Thermodynamic investigation of a new water-soluble porphyrin with calf thymus DNA" European Journal of Chemistry [Online], Volume 1 Number 4 (22 December 2010)

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