OPEN ACCESS | PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS
Rapid and sensitive determination of selective progesterone modulator ulipristal acetate in human plasma
Nagaraju Pappula (1,*) , Balaji Kodali (2) , Peda Varma Datla (3)
(1) Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Guntur-522002, Andhra Pradesh, India
(2) College of Pharmaceutical Sciences, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur (Dist), Andhra Pradesh, India
(3) Clinical Pharmacology and Bio Sciences Division, Ra Chem Pharma Ltd., Hyderabad, 500037, India
(*) Corresponding Author
Received: 12 Jun 2017 | Revised: 05 Aug 2017 | Accepted: 05 Aug 2017 | Published: 30 Sep 2017 | Issue Date: September 2017
Ulipristal acetate is a new synthetic selective progesterone receptor modulator developed mainly as emergency contraceptive (EC) and also used for the treatment of uterine fibroids. A cost effective, sensitive, simple and rapid high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the analysis of ulipristal acetate in human plasma. Following liquid-liquid extraction, the analyte (Ulipristal acetate) and internal standard (Levonorgestrel) were chromatographed using mobile phase in an isocratic elution mode on a reverse phase C18 column. The LC-MS/MS operated in multiple reaction monitoring mode for respective [M+H]+ ions, m/z 476.2/134.1 for analyte and 313.3/245.1 for internal standard. The assay exhibited linear dynamic range of 1-300 ng/mL. The lower limit of quantification was 1 ng/mL with relative standard deviation of 7.0%. The intra-batch and inter-batch results were precise with coefficient variation of 2.7 to 7.0 (%) and accuracy of 94.2-99.8 (%). The validated method was simple, fast and repeatable for bioequivalence, pharmacokinetic and therapeutic monitoring studies.
Links for Article
| | | | | | |
| | | | | | |
Article MetricsThis Abstract was viewed 954 times | PDF Article downloaded 310 times
. Ji Hyun Lee, Han Na Park, Nam Sook Kim, Hyung-Joon Park, Seongsoo Park, Dongwoo Shin, Hoil Kang
Detection of Illegal Abortion-Induced Drugs Using Rapid and Simultaneous Method for the Determination of Abortion-Induced Compounds by LC–MS/MS
Chromatographia 82(9), 1365, 2019
. Gemzell-Danielsson, K.; Chun-Xia, M. Int. J. Womens Health. 2010, 2, 53-61.
. Goldstajn, M. S.; Baldani, D. P.; Škrgatić, L.; Radaković, B.; Vrčić, H.; Čanić, T. Collegium Antropol. 2014, 38, 379-384.
. Richardson, A. R.; Fraidy, N. Clin. Ther. 2012, 1, 24-36.
. Brache, V.; Cochon, L.; Jesam, C.; Maldonado, R.; Salvatierra, A. M.; Levy, D. P.; Gainer, E.; Croxatto, H. B. Hum. Reprod. 2010, 25, 2256-2263.
. Pohl, O.; Zobrist, R. H.; Gotteland, J. P. Reprod. Sci. 2015, 22, 476-483.
. Jadav, S. P.; Parmar, D. M. J. Pharmacol. Pharmacother. 2012, 3(2), 109-111.
. Shi, J. X.; Zhu, F. Y.; Zou, Q. G.; Sun, L. L; Wei, P. Chin. J. New Drugs 2014, 7, 839-852.
. Pohl, O.; Williams, A. R.; Bergeron, C. B.; Gotteland, J. P. Regul. Toxicol. Pharmacol. 2013, 66, 6-12.
. Guidance for Industry: Bioanalytical method validation, U. S. Department of Health and Human services, Food and Drug administration, May 2001, Rockville, MD, USA.
. Bioanalytical method validation guideline, Brazil, Agenda Nacionaldevigilancia Sanitaria (ANVISA) Resolution-RDC no. 27, May 17, 2012.
. Ye, J. H.; Pao, L. H. PLoS One 2014, 24, 1-18.
. Ismaiel, O. A.; Halquist, M. S.; Elmamly, M. Y.; Shalaby, A.; Karnes, H. T. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 2007, 859, 84-93.
. www.medicines.org.uk/emc/medicine/26068/SPC/#PHARMACOKINETIC_PROPS viewed 05 August 2017.
How to cite
The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item
DOI Link: https://doi.org/10.5155/eurjchem.8.3.258-264.1596
| | | | | | |
| | | | | |
Save to Zotero Save to Mendeley
European Journal of Chemistry 2017, 8(3), 258-264 | doi: https://doi.org/10.5155/eurjchem.8.3.258-264.1596 | Get rights and content
- There are currently no refbacks.
© Copyright 2010 - 2020 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2010-2020 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.