Utilization of UPLC-tandem mass spectrometry for quantitation of colouring matters in dietary formulation

Wafaa Abdou Zaghary; Ahmed Mostafa Abdalla; Emily Tawfik Hanna; Marwa Abdel Rahman Tohamy Zanoun

doi:10.5155/eurjchem.10.3.239-243.1786

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Published: Sep 30, 2019

DOI: https://doi.org/10.5155/eurjchem.10.3.239-243.1786

Keywords:

UPLC, Limit of detection, Colouring matters, Limit of quantitation, Multiple reaction monitoring, Tandem mass spectrometric technique

Section

Research Article

Issue

Vol. 10 No. 3 (2019): September 2019

Dates

Received 2018-09-12
Accepted 2019-05-15
Published 2019-09-30

Wafaa Abdou Zaghary

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt

http://orcid.org/0000-0002-9037-9696

Ahmed Mostafa Abdalla

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt

http://orcid.org/0000-0003-4059-6390

Emily Tawfik Hanna

Consultant Nutrition Biochemistry and Metabolism Department, National Nutrition Institute, Cairo, 11562, Egypt

http://orcid.org/0000-0003-4559-7691

Marwa Abdel Rahman Tohamy Zanoun

Consultant Nutrition Biochemistry and Metabolism Department, National Nutrition Institute, Cairo, 11562, Egypt

http://orcid.org/0000-0001-7221-7071

Abstract

A simple and sensitive ultra-performance liquid chromatography with Tandem Mass Spectrometric detection (UPLC-MS/MS) method was developed and validated for the simultaneous quantitation of two food colouring matters, Curcumin (CUR) and Riboflavin (RIB). Chromatographic separation was done on Hypersil gold 50×2.1 mm (1.9 μm) column, with gradient programing of mobile phase starting with aqueous 0.1% formic acid and increasing the percentage of 0.1% formic acid in acetonitrile. The utilization of multiple reaction monitoring (MRM) improved the selectivity of detection and decreased the matrix effect. The method was linear in the range of 5-500 ng/mL for CUR and RIB. Intra- and inter-day reproducibility were within the accepted criteria. The method was successfully applied for the determination the laboratory prepared mixtures of the two selected colouring matters. The use of mass spectrometry enhanced the selectivity and sensitivity of detection which allows the robust use of the method in routine quality control tests of the two colouring matters (CUR and RIB).

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How to Cite

(1)

Zaghary, W. A.; Abdalla, A. M.; Hanna, E. T.; Zanoun, M. A. R. T. Utilization of UPLC-Tandem Mass Spectrometry for Quantitation of Colouring Matters in Dietary Formulation. Eur. J. Chem. 2019, 10, 239-243.

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Supporting Agencies

The Pharmaceutical Services Centre and LC-MS Unit, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt.

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