OPEN ACCESS | PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS
Utilization of UPLC-tandem mass spectrometry for quantitation of colouring matters in dietary formulation
Wafaa Abdou Zaghary (1) , Ahmed Mostafa Abdalla (2,*) , Emily Tawfik Hanna (3) , Marwa Abdel Rahman Tohamy Zanoun (4)
(1) Pharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt
(2) Pharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt
(3) Consultant Nutrition Biochemistry and Metabolism Department, National Nutrition Institute, Cairo, 11562, Egypt
(4) Consultant Nutrition Biochemistry and Metabolism Department, National Nutrition Institute, Cairo, 11562, Egypt
(*) Corresponding Author
Received: 12 Sep 2018 | Revised: 24 Dec 2018 | Accepted: 15 May 2019 | Published: 30 Sep 2019 | Issue Date: September 2019
A simple and sensitive ultra-performance liquid chromatography with Tandem Mass Spectrometric detection (UPLC-MS/MS) method was developed and validated for the simultaneous quantitation of two food colouring matters, Curcumin (CUR) and Riboflavin (RIB). Chromatographic separation was done on Hypersil gold 50×2.1 mm (1.9 μm) column, with gradient programing of mobile phase starting with aqueous 0.1% formic acid and increasing the percentage of 0.1% formic acid in acetonitrile. The utilization of multiple reaction monitoring (MRM) improved the selectivity of detection and decreased the matrix effect. The method was linear in the range of 5-500 ng/mL for CUR and RIB. Intra- and inter-day reproducibility were within the accepted criteria. The method was successfully applied for the determination the laboratory prepared mixtures of the two selected colouring matters. The use of mass spectrometry enhanced the selectivity and sensitivity of detection which allows the robust use of the method in routine quality control tests of the two colouring matters (CUR and RIB).
Our editors have decided to support scientists to publish their manuscripts in European Journal of Chemistry without any financial constraints.
1- The article processing fee will not be charged from the articles containing the single-crystal structure characterization or a DFT study between September 15, 2023 and October 31, 2023 (Voucher code: FALL2023).
2. A 50% discount will be applied to the article processing fee for submissions made between September 15, 2023 and October 31, 2023 by authors who have at least one publication in the European Journal of Chemistry (Voucher code: AUTHOR-3-2023).
3. Young writers will not be charged for the article processing fee between September 15, 2023 and October 31, 2023 (Voucher code: YOUNG2023).
European Journal of Chemistry
Links for Article
| | | | | | |
| | | | | | |
| | | |
Article MetricsThis Abstract was viewed 912 times | PDF Article downloaded 439 times
The Pharmaceutical Services Centre and LC-MS Unit, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt.
. Tada, A.; Jin, Z. L.; Sugimoto, N.; Sato, K.; Yamazaki, T.; Tanamoto, K. Food Hyg. Safe. Sci. 2005, 46(5), 198-204.
. Jasim, F.; Ali, F. Microchem. J. 1988, 38(1), 106-110.
. Lopez-de-Alba, P. L.; Lopez-Martinez, L.; Cerda, V.; Amador-Hernandez, J. J. Brazil. Chem. Soc. 2006, 17, 715-722.
. Garcia, L.; Blazquez, S.; San-Andres, M. P.; Vera, S. Anal. Chim. Acta 2001, 434(2), 193-199.
. Petteys, B. J.; Frank, E. L. Clin. Chim. Acta 2011, 412(1), 38-43.
. Tang, X.; Cronin, D. A.; Brunton, N. P. J. Food Compos. Anal. 2006, 19(8), 831-837.
. Jadhav, B. K.; Mahadik, K. R.; Paradkar, A. R. Chromatographia 2007, 65(7), 483-488.
. Jayaprakasha, G. K.; Jagan, M. R. L.; Sakariah, K. K. J. Agr. Food Chem. 2002, 50(13), 3668-3672.
. Berset, J. D.; Ochsenbein, N. Chemosphere 2012, 88(5), 563-569.
. Chen, X. H.; Zhao, Y. G.; Shen, H. Y.; Jin, M. C. J. Chromatogr. A 2012, 1263, 34-42.
. Vallverdu-Queralt, A.; Regueiro, J.; Martinez-Huelamo, M.; Rinaldi, A. J. F.; Leal, L. N.; Lamuela-Raventos, R. M. Food Chem. 2014, 154, 299-307.
. ICH Harmonized Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology, Q2(R1).. Current Step 4 Version, 2005.
. Shrivastava, A.; Gupta, V. B. Chron. Young. Sci. SP. 2011, 2(1), 21-25.
How to cite
The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item
DOI Link: https://doi.org/10.5155/eurjchem.10.3.239-243.1786
| | | | | | | |
| | | | | | |
Save to Zotero Save to Mendeley
European Journal of Chemistry 2019, 10(3), 239-243 | doi: https://doi.org/10.5155/eurjchem.10.3.239-243.1786 | Get rights and content
- There are currently no refbacks.
Copyright (c) 2019 Authors
This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at http://www.eurjchem.com/index.php/eurjchem/pages/view/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (http://www.eurjchem.com/index.php/eurjchem/pages/view/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).
© Copyright 2010 - 2023 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2010-2023 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.