European Journal of Chemistry

RP-HPLC method development and validation for simultaneous estimation of ramipril and felodipine


Main Article Content

Elham Anwar Taha
Manal Mohammed Fouad
Ali Kamal Attia
Zainab Mahmoud Yousef


A rapid and sensitive High Performance Liquid Chromatography (HPLC) method has been developed and validated as per ICH guideline for simultaneous determination of ramipril and felodipine binary mixture. Chromatographic separation was achieved on a Hyperchom C18 column (250 × 4.6 mm i.d., 5 μm) using an isocratic mobile phase of potassium di-hydrogen phosphate (pH = 3.4): methanol: acetonitrile in the ratio 15:15:70 (v:v:v). The flow rate was 1.5 mL/min, temperature of the column was maintained at 30 °C and detection was made at 210 nm. Linearity studies indicated that the drugs obey Beer’s law over the range of 10-80 μg/mL for ramipril and 5-80 μg/mL for felodipine. The proposed method is precise, accurate, linear and robust. The short retention time allows the analysis of a large number of samples in a short period of time and, therefore, considered to be cost-effective that can be used for routine analysis of both drugs in the pharmaceutical industry.

icon graph This Abstract was viewed 1866 times | icon graph Article PDF downloaded 576 times

How to Cite
Taha, E. A.; Fouad, M. M.; Attia, A. K.; Yousef, Z. M. RP-HPLC Method Development and Validation for Simultaneous Estimation of Ramipril and Felodipine. Eur. J. Chem. 2019, 10, 113-117.

Article Details

Crossref - Scopus - Google - European PMC

[1]. Raja, A. P.; Venkateshwar, R. J. Asian J. Pharm. Clin. Res. 2013, 6(3), 178-181.

[2]. British Pharmacopoeia. 9th edition, The Council of Europe: 2018; Vol. I 768-771& II 1003-1005.

[3]. Brunton, L.L.; Goodman and Gilman's., 2011. The Pharmacological Basis of Therapeutics, The McGraw-Hill Co., Inc., China.

[4]. Whalen, K., Finkel, R., Panavelil, T.A., 2015. Lippincott's Illustrated Reviews: Pharmacology, Lippincott Williams and Wilkins, Philadelphia, Baltimore, New York, London.

[5]. Salama, F. M.; El-Sattar, O. I. A.; El-Aba, S. N. M.; Fuad, M. M. Al-Azhar J. Pharmaceu. Sci. 2001, 27, 121-132.

[6]. Ustun, F. G.; Ustun, O.; Atay, O. Turk. J. Pharm. Sci. 2004, 2(1), 65-76.

[7]. Rahman, N.; Ahmad, Y.; Azmi, S. N. H. AAPS Pharm. Sci. Tech. 2005, 6(3), E543-E551.

[8]. Patil, K. R.; Rane, V. P.; Sangshetti, J. N.; Shinde, D. B. Chromatographia 2008, 67(7-8), 575-582.

[9]. Rajput, P. S.; Kaur, A.; Gill, N. K.; Mittal, K.; Sarma, G. S. J. Appl. Pharm. Sci. 2012, 2(7), 160-165.

[10]. Szpot, P.; Buszewicz, G. Acta Pharmaceut. 2015, 65(2), 159-169.

[11]. Patel, A. V.; Patel, P.; Chaudhary, B.; Rajgor, N.; Rathi, S. Int. J. Biol. Pharm. Res. 2010, 1(1), 18-24.

[12]. Parmar, D. A.; V. Thakkar, D.; Patel, R. B.; Patel, M. R. Thai J. Pharm. Sci. 2015, 39(3), 83-88.

[13]. Belal, F.; Al-Zaagi, I. A.; Abounassif, M. A. J. AOAC Int. 2001, 84(1), 1-8.

[14]. Prieto, J. A.; Jimenez, R. M.; Alonso, R. M. Il Farmaco 2003, 58(5), 343-350.

[15]. Mattos, G. J.; Scremin, J.; Salamanca-Neto, C. A. R.; Sartori, E. R. Electroanalysis 2017, 29(4), 1180-1187.

[16]. Silva, T. A.; Fatibello-Filho, O. Anal. Methods-UK 2017, 9(32), 4680-4687.

[17]. Basavaiah, K.; Chandrashekar, U.; Prameela, H. C. Il Farmaco 2003, 58(2), 141-148.

[18]. Salem, H.; Abdallah, O. M. Am. J. Appl. Sci. 2007, 4(9), 709-717.

[19]. Nimje, H.; Oswal, R.; Kshirsagar, S. S.; Chavan, M. Res. J. Pharm. Tech. 2011, 4(12), 1805-1806.

[20]. Jadhav, N. R.; Kambar, R. S.; Nadaf, S. Adv. Chem. 2014, Article ID 131974, 1-6.

[21]. Karlsson, A.; Pettersson, K.; Hernqvist, K. Chirality 1995, 7(3), 147-153.

[22]. Cardoza, R.; Amin, P. J. Pharmaceut. Biomed. 2002, 27(5), 711-718.

[23]. Baranda, A.B.; Jimenez, R. M.; Alonso, R. M. J. Chromatogr. A 2004, 1031(1-2), 275-280.

[24]. Walash, M. I.; Belal, F. F.; El-Enany, N. M.; El-Maghrabey, M. H. Chem. Cent. J. 2011, 5, 61, 1-11.

[25]. Dru, J. D. Y.; Hsieh, J. Y. K.; Matuszewski, B. K.; Dobrinska, M. R. J. Chromatogr. B 1995, 666(2), 259-267.

[26]. Jammal, A. E.; Vire, J. C.; Patriarche, G. J.; Palmeiro, O. N. Electroanalysis 1992, 4(1), 57-64.

[27]. Sikkander, A. R. M.; Vedhi, C.; Manisankar, P. Int. J. Indust. Chem. 2012, 3(1), 29-37.

[28]. Rontogianni, M. A.; Markopoulou, C. K.; Koundourellis, J. E. J. Liq. Chromatogr. 2006, 29(18), 2701-2719.

[29]. El-Yazbi, F. A.; Mahrous, M. E.; Hammud, H. H.; Sonji, G. M.; Sonji, N. M. Anal. Lett. 2008, 41(5), 853-870.

[30]. B. Raja; Rao, A. L. Asian J. Res. Chem. 2013, 6(11), 1018-1022.

[31]. Gawai, A. A.; Shaikh, T.; Kolhe, S.; Shaikh, F.; Deokar, N. Int. J. Chem. Tech. Res. 2018, 11(2), 228-239.

[32]. Mohamed, A. M.; Omar, M. A.; Hammad, M. A.; Mohamed, A. A. Biomed. Chromatogr. 2016, 30(2), 200-207.

[33]. ICH Harmonised Tripartite Guideline. USA, 2005.

[34]. Miller, J. N.; Miller, J. C. Statistics and Chemometrics for Analytical Chemistry. 6th edition, Pearson Education Limited: Harlow, England, 2010.


Dimensions - Altmetric - scite_ - PlumX

Downloads and views


Download data is not yet available.


Metrics Loading ...
License Terms

License Terms


Copyright © 2024 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License ( By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License ( are administered by Atlanta Publishing House LLC (European Journal of Chemistry).