European Journal of Chemistry 2019, 10(2), 113-117 | doi: | Get rights and content


RP-HPLC method development and validation for simultaneous estimation of ramipril and felodipine

Elham Anwar Taha (1) orcid , Manal Mohammed Fouad (2,*) orcid , Ali Kamal Attia (3) orcid , Zainab Mahmoud Yousef (4) orcid

(1) National Organization for Drug Control and Research (NODCAR), Giza, 12553, Egypt
(2) Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, 11651, Egypt
(3) National Organization for Drug Control and Research (NODCAR), Giza, 12553, Egypt
(4) National Organization for Drug Control and Research (NODCAR), Giza, 12553, Egypt
(*) Corresponding Author

Received: 09 Feb 2019 | Revised: 20 Apr 2019 | Accepted: 04 May 2019 | Published: 30 Jun 2019 | Issue Date: June 2019


A rapid and sensitive High Performance Liquid Chromatography (HPLC) method has been developed and validated as per ICH guideline for simultaneous determination of ramipril and felodipine binary mixture. Chromatographic separation was achieved on a Hyperchom C18 column (250 × 4.6 mm i.d., 5 μm) using an isocratic mobile phase of potassium di-hydrogen phosphate (pH = 3.4): methanol: acetonitrile in the ratio 15:15:70 (v:v:v). The flow rate was 1.5 mL/min, temperature of the column was maintained at 30 °C and detection was made at 210 nm. Linearity studies indicated that the drugs obey Beer’s law over the range of 10-80 μg/mL for ramipril and 5-80 μg/mL for felodipine. The proposed method is precise, accurate, linear and robust. The short retention time allows the analysis of a large number of samples in a short period of time and, therefore, considered to be cost-effective that can be used for routine analysis of both drugs in the pharmaceutical industry.


Isocratic; Ramipril; Felodipine; Routine analysis; Pharmaceuticals; Chromatography

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DOI: 10.5155/eurjchem.10.2.113-117.1832

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National Organization for Drug Control and Research (NODCAR), Egypt.


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How to cite

Taha, E.; Fouad, M.; Attia, A.; Yousef, Z. Eur. J. Chem. 2019, 10(2), 113-117. doi:10.5155/eurjchem.10.2.113-117.1832
Taha, E.; Fouad, M.; Attia, A.; Yousef, Z. RP-HPLC method development and validation for simultaneous estimation of ramipril and felodipine. Eur. J. Chem. 2019, 10(2), 113-117. doi:10.5155/eurjchem.10.2.113-117.1832
Taha, E., Fouad, M., Attia, A., & Yousef, Z. (2019). RP-HPLC method development and validation for simultaneous estimation of ramipril and felodipine. European Journal of Chemistry, 10(2), 113-117. doi:10.5155/eurjchem.10.2.113-117.1832
Taha, Elham, Manal Mohammed Fouad, Ali Kamal Attia, & Zainab Mahmoud Yousef. "RP-HPLC method development and validation for simultaneous estimation of ramipril and felodipine." European Journal of Chemistry [Online], 10.2 (2019): 113-117. Web. 6 Aug. 2020
Taha, Elham, Fouad, Manal, Attia, Ali, AND Yousef, Zainab. "RP-HPLC method development and validation for simultaneous estimation of ramipril and felodipine" European Journal of Chemistry [Online], Volume 10 Number 2 (30 June 2019)

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