

Spectroscopic and molecular docking elucidation to binding characteristics of bovine serum albumin with bupropion an aminoketone-medication for nicotine addiction
Manjushree Makegowda (1)


(1) Department of Chemistry, University of Mysore, Manasagangothri, Mysuru 570 006, Karnataka, India
(2) Department of Chemistry, University of Mysore, Manasagangothri, Mysuru 570 006, Karnataka, India
(*) Corresponding Author
Received: 08 Mar 2019 | Revised: 29 Apr 2019 | Accepted: 04 May 2019 | Published: 30 Jun 2019 | Issue Date: June 2019
Abstract
One of the highly soluble protein presents in circulatory system of bovine body is bovine serum albumin (BSA). Bupropion hydrochloride (BRN) served to treat prime smoking cessation and disorder due to depressive. BRN binding to BSA was studied by molecular docking and lots of spectroscopic (UV-vis, emission, synchronous, 3D fluorescence, CD and FT-IR) methods at pH = 7.40. Static quenching with strong binding was obtained for BSA-BRN system by forming complex. Secondary structures, conformations and microenvironments of BSA were altered after BRN interaction. Distance between BRN and BSA was also achieved. Biologically active metal ions (Cu2+, Ca2+, Mg2+, Fe2+ and Zn2+) were also influenced on the BSA-BRN complex. Bonds of hydrogen and Van der Waals were major binding forces to stabilize BSA-BRN complex at site I (IIA) of BSA. Hence, binding of BRN to transport protein (BSA) is of prominent importance and these findings could be helpful for BRN pharmacology and potential clinical research.
Announcements
One of our sponsors will cover the article processing fee for all submissions made between May 17, 2023 and June 16, 2023 (Voucher code: SPONSOR2023).
Editor-in-Chief
European Journal of Chemistry
Keywords
Full Text:
PDF

DOI: 10.5155/eurjchem.10.2.146-155.1845
Links for Article
| | | | | | |
| | | | | | |
| | | |
Related Articles
Article Metrics


Funding information
University Grants Commission of Basic Scientific Research section (UGC-BSR) for awarding the fellowship under meritorious students, the CATERS CSIR-CLRI, Chennai, India.
References
[1]. Bupropion hydrochloride, RxList. https://www.rxlist.com/ wellbutrin-xl-drug.htm/ , 2018 (accessed 06 September 2018).
[2]. Bupropion hydrochloride, WebMD. https://www.webmd.com/ drugs/2/drug-13507-7155/bupropion-hcl-oral/bupropion-extended-release-antidepressant-oral/details/, 2018 (accessed 06 September 2018).
[3]. Zhang, G.; Wang, L.; Fu, P.; Hu, M. Spectrochim. Acta A 2011, 82, 424-431.
https://doi.org/10.1016/j.saa.2011.07.073
[4]. Zhang, Q.; Fu, X.; Garcia Martín, J. F. Ultrason. Sonochem. 2017, 37, 405-413.
https://doi.org/10.1016/j.ultsonch.2017.01.031
[5]. Manjushree, M.; Revanasiddappa, H. D. J. Mol. Liq. 2017, 237, 23-37.
[6]. Sajid Ali, M.; Al-Lohedan, H. J. Mol. Liq. 2017, 236, 232-240.
https://doi.org/10.1016/j.molliq.2017.04.020
[7]. Skrt, M.; Benedik, E.; Podlipnik, C.; Ulrih, N. P. J. Food. Chem. 2012, 135, 2418-2424.
https://doi.org/10.1016/j.foodchem.2012.06.114
[8]. Majorek, K. A.; Porebski, P. J.; A. Dayal, M. D.; Zimmerman, K.; Jablonska, A. J.; Stewart, M.; Chruszcz, W. J. Mol. Immunol. 2012, 52, 174-182.
https://doi.org/10.1016/j.molimm.2012.05.011
[9]. Ojha, B.; Das, G. J. Phys. Chem. 2010, 114, 3979-3986.
https://doi.org/10.1021/jp907576r
[10]. Manjushree, M.; Revanasiddappa, H. D. Spectrochim. Acta A 2019, 209, 264-273.
[11]. Lakowicz, J. R. Principles of Fluorescence Spectroscopy. Third ed. Springer, New York, 2006.
https://doi.org/10.1007/978-0-387-46312-4
[12]. Rosenheck, K.; Doty, P. Proc. Natl. Acad. Sci. U. S. A. 1961, 47, 1775-1785.
https://doi.org/10.1073/pnas.47.11.1775
[13]. Zhou, K.; Pan, D.; Lou, Y.; Shi, J. J. Mol. Recognit. 2018, e2716.
https://doi.org/10.1002/jmr.2716
[14]. Sun, Z.; Xu, H.; Cao, Y.; Wang, F.; Mi, W. J. Mol. Liq. 2016, 219, 405-410.
https://doi.org/10.1016/j.molliq.2016.03.040
[15]. Lakowicz, J. R.; Weber, G. Biochemistry 1973, 12, 4161-4170.
https://doi.org/10.1021/bi00745a020
[16]. Chaves, O. A.; Silva, V. A.; Sant'Anna, C. M. R.; Ferreira, A. B. B.; Ribeiro, T. A. N.; Carvalho, M. G.; Cesarin-Sobrinho, D.; Netto-Ferreira, J. C. J. Mol. Struct. 2017, 1128, 606-611.
https://doi.org/10.1016/j.molstruc.2016.09.036
[17]. Wei, X. L.; Xiao, J. B.; Wang, Y.; Bai, Y. Spectrochim. Acta A. 2010, 75, 299-304.
https://doi.org/10.1016/j.saa.2009.10.027
[18]. Ross, P. D.; Subramanian, S. Biochemistry 1981, 20, 3096-3102.
https://doi.org/10.1021/bi00514a017
[19]. Xi, J. Q.; Fan, L. J. Therm. Anal. Calorim. 2010, 102, 217-223.
https://doi.org/10.1007/s10973-010-0792-y
[20]. Katrahalli, U.; Jaldappagari, S.; Kalanur, S. J. Lumin. 2010, 130, 211-216.
https://doi.org/10.1016/j.jlumin.2009.07.033
[21]. Yang, P.; Gao, F. The Principle of Bioinorganic Chemistry. Science Press: Beijing, China, 2002, pp. 349.
[22]. Förster, T. Delocalized excitation and excitation transfer, O. Sinanoglu (Ed.), Modern Quantum Chemistry. Istanbul Lectures 3, Academic Press, New York/London, 1965, pp. 93-137.
[23]. Zhang, Q.; Ni, Y.; Kokot, S. J. Pharm. Biomed. Anal. 2010, 52, 280-288.
https://doi.org/10.1016/j.jpba.2010.01.006
[24]. Molina-Bolívar, J. A.; Galisteo-González, F.; Ruiz, C. C.; Donnell, M. M.; Parra, A. J. Mol. Liq. 2015, 208, 304-313.
https://doi.org/10.1016/j.molliq.2015.04.050
[25]. Ni, Y.; Zhu, R.; Kokot, S. Analyst 2011, 136, 4794-4801.
https://doi.org/10.1039/c1an15550d
[26]. Wang, N.; Ye, L.; Yan, F.; Xu, R. Int. J. Pharm., 2008, 351, 55-60.
https://doi.org/10.1016/j.ijpharm.2007.09.016
[27]. Xiao, J. B.; Chen, J. W.; Cao, H.; Ren, F. L.; Yang, C. S.; Chen, Y.; Xu, M. J. Photochem. Photobiol. A 2007, 191, 222-227.
https://doi.org/10.1016/j.jphotochem.2007.04.027
Supporting information
The Supplementary Material for this article can be found online at: Supplementary files
How to cite
The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item
DOI Link: https://doi.org/10.5155/eurjchem.10.2.146-155.1845

















European Journal of Chemistry 2019, 10(2), 146-155 | doi: https://doi.org/10.5155/eurjchem.10.2.146-155.1845 | Get rights and content
Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Authors

This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at http://www.eurjchem.com/index.php/eurjchem/pages/view/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (http://www.eurjchem.com/index.php/eurjchem/pages/view/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).
© Copyright 2010 - 2023 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2010-2023 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.