European Journal of Chemistry

Crystallization of 3-hexulose-6-phosphate synthase


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Masoud Delfi
Leila Mahdavian
Mohammad Sattarifar
Nina Hakulinen
Juha Rouvinen


The crystal structures can reveal detailed information about the overall structure, active site structure, and functional mechanism of enzymes. This study focused on the crystallization of 3-hexulose-6-phosphate synthase from Methylomonas aminofaciens 77a, to produce higher resolution crystals for precise structural characterization. 3-Hexulose-6-phosphate synthase is from Methylomonas aminofaciens 77a (EC It belongs to the orotidine 5'-monophosphate decarboxylase superfamily, and acts as a key enzyme for a ribulose-monophosphate cycle of formaldehyde fixation and detoxification. 3-Hexulose-6-phosphate synthase catalyzes the aldol condensation of formaldehyde with D-ribulose-5-phosphate. For the maximum activity, 3-hexulose-6-phosphate synthase requires Mg2+ or Mn2+ as ligands. MaHPS crystallized at the concentration of 7 mg/mL and conditions consisting of 0.2 M MgCl2, 18% PEG 3350 at pH = 7.0.

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How to Cite
Delfi, M.; Mahdavian, L.; Sattarifar, M.; Hakulinen, N.; Rouvinen, J. Crystallization of 3-Hexulose-6-Phosphate Synthase. Eur. J. Chem. 2021, 12, 299-303.

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[1]. Stolzenberger, J.; Lindner, S. N.; Wendisch, V. F. Microbiology 2013, 159 (Pt 8), 1770-1781.

[2]. Kato, N.; Yurimoto, H.; Thauer, R. K. Biosci. Biotechnol. Biochem. 2006, 70 (1), 10-21.

[3]. Zhang, W.; Zhang, T.; Wu, S.; Wu, M.; Xin, F.; Dong, W.; Ma, J.; Zhang, M.; Jiang, M. RSC Adv. 2017, 7 (7), 4083-4091.

[4]. Park, Y.; Yoo, H.; Song, M.; Lee, D.-H.; Lee, S. Catalysts 2018, 8 (12), 582.

[5]. Orita, I.; Kita, A.; Yurimoto, H.; Kato, N.; Sakai, Y.; Miki, K. Proteins 2010, 78 (16), 3488-3492.

[6]. Rahman, M. M.; Andberg, M.; Koivula, A.; Rouvinen, J.; Hakulinen, N. Sci. Rep. 2018, 8 (1), 865.

[7]. Witthoff, S.; Schmitz, K.; Niedenführ, S.; Nöh, K.; Noack, S.; Bott, M.; Marienhagen, J. Appl. Environ. Microbiol. 2015, 81 (6), 2215-2225.

[8]. Lee, J. C.; Herman, P. Methods Enzymol. 2011, 488, 185-217.

[9]. Kato, N.; Ohashi, H.; Hori, T.; Tani, Y.; Ogata, K. Agric. Biol. Chem. 1977, 41 (7), 1133-1140.

[10]. Khurshid, S.; Saridakis, E.; Govada, L.; Chayen, N. E. Nat. Protoc. 2014, 9 (7), 1621-1633.

[11]. Price, J. V.; Chen, L.; Whitaker, W. B.; Papoutsakis, E.; Chen, W. Proc. Natl. Acad. Sci. U. S. A. 2016, 113 (45), 12691-12696.

[12]. Yew, W. S.; Wise, E. L.; Rayment, I.; Gerlt, J. A. Biochemistry 2004, 43 (21), 6427-6437.

[13]. Wise, E.; Yew, W. S.; Babbitt, P. C.; Gerlt, J. A.; Rayment, I. Biochemistry 2002, 41 (12), 3861-3869.

[14]. Yew, W. S.; Akana, J.; Wise, E. L.; Rayment, I.; Gerlt, J. A. Biochemistry 2005, 44 (6), 1807-1815.

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Department of Chemistry, University of Eastern Finland, Joensuu Campus, Joensuu, FIN-80101 Finland.
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