European Journal of Chemistry 2011, 2(3), 372-377. doi:10.5155/eurjchem.2.3.372-377.396

Development and validation of novel analytical methods for estimation of doxofylline in bulk and dosage forms

Atkuru Veera Venkata Naga Krishna Sunil (1,*) , Settaluri Vijaya Saradhi (2) , Chandra Bala Sekaran (3) , Tamanampudi Varahala Reddy (4)

(1) Department of Chemistry, NRI College, Vijayawada, 520 010, India
(2) Department of Biotechnology, Koneru Lakshmaiah University, Guntur district, 522 502, India
(3) Department of Biotechnology, Jagarlamudi Kuppuswamy Choudary College, Guntur, 522 006, India
(4) Department of Chemistry, Parvathaneni Brahmayya Siddhartha College of Arts and Sciences, Vijayawada, 520 010, India
(*) Corresponding Author

Received: 27 Jan 2011, Accepted: 24 Feb 2011, Published: 30 Sep 2011


Three simple, sensitive and accurate spectrophotometric methods (A, B and C) were developed to determine doxofylline in bulk and in its dosage forms. Method A is based on charge-transfer complex formation of the drug with p-chloranilic acid. Method B involves the formation of colored chloroform extractable ion-pair complex of the drug with bromophenol blue under acidic condition. Method C is based on ternary complex formation of the drug with molybdenum(V) thiocyanate binary complex. The colored products are quantitated spectrophotometrically at 540, 390 and 690 nm by methods A, B and C, respectively. The variables, which affected the reactions were carefully studied and optimized. The linear ranges for the proposed methods were 4-32 μg/mL (method A & B) and 2-16 μg/mL (method C). Analytical performance of the methods was statistically validated. The proposed methods were successfully applied to the analysis of doxofylline in its dosage forms and were found to be comparable with that of reference method.



Doxofylline; Ion association complex; Charge transfer complex; Ternary complex; Spectrophotometric analysis; Doxofylline analysis

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How to cite

Sunil, A.; Saradhi, S.; Sekaran, C.; Reddy, T. Eur. J. Chem. 2011, 2(3), 372-377. doi:10.5155/eurjchem.2.3.372-377.396
Sunil, A.; Saradhi, S.; Sekaran, C.; Reddy, T. Development and validation of novel analytical methods for estimation of doxofylline in bulk and dosage forms. Eur. J. Chem. 2011, 2(3), 372-377. doi:10.5155/eurjchem.2.3.372-377.396
Sunil, A., Saradhi, S., Sekaran, C., & Reddy, T. (2011). Development and validation of novel analytical methods for estimation of doxofylline in bulk and dosage forms. European Journal of Chemistry, 2(3), 372-377. doi:10.5155/eurjchem.2.3.372-377.396
Sunil, Atkuru, Settaluri Vijaya Saradhi, Chandra Bala Sekaran, & Tamanampudi Varahala Reddy. "Development and validation of novel analytical methods for estimation of doxofylline in bulk and dosage forms." European Journal of Chemistry [Online], 2.3 (2011): 372-377. Web. 22 Sep. 2019
Sunil, Atkuru, Saradhi, Settaluri, Sekaran, Chandra, AND Reddy, Tamanampudi. "Development and validation of novel analytical methods for estimation of doxofylline in bulk and dosage forms" European Journal of Chemistry [Online], Volume 2 Number 3 (30 September 2011)

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