European Journal of Chemistry 2011, 2(4), 524-534. doi:10.5155/eurjchem.2.4.524-534.438

A quick method for surveillance of 59 pesticide residues in fruits and vegetables using rapid three-dimensional gas chromatography (GC/MSD/µ-ECD/FPD) and LC/MS-MS


Gouri Satpathy (1) , Yogesh Kumar Tyagi (2,*) , Rajinder Kumar Gupta (3)

(1) University School of Basic and Applied Sciences, Guru Gobind Singh Indraprastha University, Sector 16 C, Dwarka, Delhi-110075, India
(2) University School of Basic and Applied Sciences, Guru Gobind Singh Indraprastha University, Sector 16 C, Dwarka, Delhi-110075, India
(3) University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector 16 C, Dwarka, Delhi-110075, India
(*) Corresponding Author

Received: 03 Apr 2011, Accepted: 23 May 2011, Published: 31 Dec 2011

Abstract


This article describes a simple, quick and inexpensive method for determination of pesticides in fruits and vegetables. The method, known as the quick, easy, cheap, effective, rugged and safe (QuEChERS) method for pesticide residues, involves the extraction and simultaneous liquid-liquid partitioning formed by adding anhydrous magnesium sulfate (MgSO4) plus sodium acetate (NaAc) followed by a simple cleanup step known as dispersive solid-phase extraction (dSPE). The extracts were analyzed by three-dimensional gas chromatography GC/MSD/µ-ECD/FPD in trace ion mode and liquid chromatography/tandem mass spectrometry (LC/MS-MS). Method sensitivity, linearity, repeatability and reproducibility, accuracy, matrix effects, and overall uncertainties have been studied for method validation according to the international norm ISO/IEC: 17025:2005 for both techniques. Identification, quantification and reporting with Total and Extracted ion chromatograms, µECD and DFPD were facilitated to a great extent by Deconvolution Reporting Software (DRS) for GC and Mass hunter software for LC. For all compounds LODs were 0.001 to 0.01 mg/kg and LOQs were 0.005 to 0.020 mg/kg. Correlation coefficients of the calibration curves were >0.991. To validate the effects of matrices, repeatability, reproducibility, recovery and overall uncertainty were calculated for twenty-four matrices at 0.020, 0.050 and 0.500 mg/kg. Recovery ranged between 75-107 % with RSD <17 % for repeatability and intermediate precision and UM of ± 13-22 %.

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Keywords


Fruits and vegetables; Pesticides; QuEChERS; Method validation; GC-MSD-µECD-DFPD; LC-MS/MS

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DOI: 10.5155/eurjchem.2.4.524-534.438

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How to cite


Satpathy, G.; Tyagi, Y.; Gupta, R. Eur. J. Chem. 2011, 2(4), 524-534. doi:10.5155/eurjchem.2.4.524-534.438
Satpathy, G.; Tyagi, Y.; Gupta, R. A quick method for surveillance of 59 pesticide residues in fruits and vegetables using rapid three-dimensional gas chromatography (GC/MSD/µ-ECD/FPD) and LC/MS-MS. Eur. J. Chem. 2011, 2(4), 524-534. doi:10.5155/eurjchem.2.4.524-534.438
Satpathy, G., Tyagi, Y., & Gupta, R. (2011). A quick method for surveillance of 59 pesticide residues in fruits and vegetables using rapid three-dimensional gas chromatography (GC/MSD/µ-ECD/FPD) and LC/MS-MS. European Journal of Chemistry, 2(4), 524-534. doi:10.5155/eurjchem.2.4.524-534.438
Satpathy, Gouri, Yogesh Kumar Tyagi, & Rajinder Kumar Gupta. "A quick method for surveillance of 59 pesticide residues in fruits and vegetables using rapid three-dimensional gas chromatography (GC/MSD/µ-ECD/FPD) and LC/MS-MS." European Journal of Chemistry [Online], 2.4 (2011): 524-534. Web. 17 Sep. 2019
Satpathy, Gouri, Tyagi, Yogesh, AND Gupta, Rajinder. "A quick method for surveillance of 59 pesticide residues in fruits and vegetables using rapid three-dimensional gas chromatography (GC/MSD/µ-ECD/FPD) and LC/MS-MS" European Journal of Chemistry [Online], Volume 2 Number 4 (31 December 2011)

DOI Link: https://doi.org/10.5155/eurjchem.2.4.524-534.438

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