European Journal of Chemistry 2010, 1(2), 110-114 | doi: https://doi.org/10.5155/eurjchem.1.2.110-114.74 | Get rights and content

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The cytotoxic activity of Linum grandiflorum leaves


Magdy Mostafa Desoky Mohammed (1,*) , Ming Chen (2) , Lin Zhai (3) , Nabaweya Ali Ibrahim (4)

(1) Danish Institute of Agricultural Sciences, Department of Food Science, Research Center Aarslev, Kirstinebjergvej 10, DK-5792 Aarslev, Denmark
(2) Department of Clinical Microbiology, University Hospital of Copenhagen (Rigshospitalet), Tagensvej 20, DK-2200 Copenhagen, Denmark
(3) Department of Clinical Microbiology, University Hospital of Copenhagen (Rigshospitalet), Tagensvej 20, DK-2200 Copenhagen, Denmark
(4) Pharmacognosy Department, National Research Center, Dokki-12311, Cairo, Egypt
(*) Corresponding Author

Received: 26 Apr 2010 | Accepted: 03 May 2010 | Published: 15 Jun 2010 | Issue Date: June 2010

Abstract


The CHCl3 and MeOH fractions of the leaves of Linum grandiflorum, showed cytotoxic activity against EL4 (Murine Leukemia) cell line with IC50 = 60 and 250 mg/mL respectively. Bioassay guided fractionation and isolation (BGFI) of the MeOH fraction resulted in the isolation of two new cytotoxic compounds 1 [luteolin 7-O-α-D-(6```-E-feruloyl)glucopyranosyl (1→2)-β-D-glucopyranoside] and 6 [2-[(3`-isopropoxy-O-β-D-glucopyranosyl)oxy]-2-methylbutane nitrile] against EL4 with IC50 = 0.2 and 0.3 mM/mL respectively, together with 2 [luteolin 7-O-β-D-glucopyranoside], 3 [vicenin-1], 4 [vicenin-2], 5 [vicenin-3], 7 [linamarin], 8 [lotaustralin], 9 [neolinustatin], and  10 [butan-2-O-β-D-glucopyranoside] which showed cytotoxicity against EL4 with IC50 = 0.2, 0.9, 0.8, 0.9, 0.4, 0.4, 0.2, 0.4 mM/mL respectively. BGFI of the CHCl3 fraction revealed the isolation of three cytotoxic aryltetrahydronaphthalene-type lignans identified as 11 [podophyllotoxin], 12 [deoxypodophylotoxin] and 13 [5-methoxypodophyllotoxin] against EL4 with IC50 = 0.2, 0.09 and 0.2 mM/mL respectively. The isolated compounds were structurally elucidated using 1D, 2D NMR, HR-ESI-MS, and HR-MALDI-MS techniques.

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Keywords


Linum grandiflorum Desf.; Linaceae; Flavonoids glycosides; Cyanogenic glycosides; Lignans; Cytotoxicity; Murine Leukemia (EL4)

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DOI: 10.5155/eurjchem.1.2.110-114.74

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Funding information


Danish Institute of Agricultural Sciences, Research Center Aarslev, Denmark and University of Southern Denmark, Odense, Denmark

Citations

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How to cite


Mohammed, M.; Chen, M.; Zhai, L.; Ibrahim, N. Eur. J. Chem. 2010, 1(2), 110-114. doi:10.5155/eurjchem.1.2.110-114.74
Mohammed, M.; Chen, M.; Zhai, L.; Ibrahim, N. The cytotoxic activity of Linum grandiflorum leaves. Eur. J. Chem. 2010, 1(2), 110-114. doi:10.5155/eurjchem.1.2.110-114.74
Mohammed, M., Chen, M., Zhai, L., & Ibrahim, N. (2010). The cytotoxic activity of Linum grandiflorum leaves. European Journal of Chemistry, 1(2), 110-114. doi:10.5155/eurjchem.1.2.110-114.74
Mohammed, Magdy, Ming Chen, Lin Zhai, & Nabaweya Ali Ibrahim. "The cytotoxic activity of Linum grandiflorum leaves." European Journal of Chemistry [Online], 1.2 (2010): 110-114. Web. 2 Mar. 2021
Mohammed, Magdy, Chen, Ming, Zhai, Lin, AND Ibrahim, Nabaweya. "The cytotoxic activity of Linum grandiflorum leaves" European Journal of Chemistry [Online], Volume 1 Number 2 (15 June 2010)

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