European Journal of Chemistry

A simple spectrophotometric method for determination of thiamine (vitamin B1) in pharmaceuticals

Main Article Content

Khairia Mohammed Al-Ahmary


A simple spectrophotometric method has been described for the determination of thiamine. The method is based on the precipitation of sulphur as barium sulphate using barium chloride after oxidation of thiamine by sodium hydroxide and hydrogen peroxide. Beer's law was obeyed over the concentration range from 2-32 µg/mL thiamine. The average of standard deviation was 1.18×10-2; standard error, 5.20×10-3; relative standard deviation, 0.51%; relative standard error, 5.40×10-3; 95% coefficient, 1.48×10-2; the limit of detection, 0.41 µg/mL; the limit of quantification, 1.38 µg/mL and recovery, 100.03% thiamine. Finally, the developed method was applied to the determination of thiamine in pharmaceutical formulations.

icon graph This Abstract was viewed 3349 times | icon graph Article PDF downloaded 1331 times

How to Cite
Al-Ahmary, K. M. A Simple Spectrophotometric Method for Determination of Thiamine (vitamin B1) in Pharmaceuticals. Eur. J. Chem. 2014, 5, 81-84.

Article Details

Crossref - Scopus - Google - European PMC

[1]. Perez-Ruiz, T.; Martinz-Lozano, C.; Sanz, A.; Guillen, A. Biomed. Anal. 2004, 34, 551-557.

[2]. Chin, H. B. Methods of vitamin assay, 3rd ed., Interscience Publishers, New York, 1966, Chap. 6, pp. 123-142.

[3]. Karlberg, B.; Thelender, S. Anal. Chim. Acta 1980, 114, 129-136.

[4]. Grekas, N.; Calokerinos, A. C. Talanta 1990, 37, 1043-1048.

[5]. Martinez-Lozano, C.; Perez-Ruiz, T.; Tomas, V.; Abellan, C. Analyst 1990, 115, 217-220.

[6]. Perez-Ruiz, T.; Martinez-Lozano, C.; Tomas, V.; Ibarra, I. Talanta 1992, 39, 907-911.

[7]. Calatayud, J. M.; Gomez-Bentio, C.; Gimenez, D. J. Pharm. Biomed. Anal. 1990, 8, 667-670.

[8]. Kssube, K.; Abe, K.; Ishiguro, Y.; Ishikawa, S.; Hosida, H. Chem. Pharm. Bull. 1983, 31, 3589-3594.

[9]. Danet, A. F.; Calatayud, J. M. Talanta 1994, 41, 2147-2151.

[10]. Chen, H.; Cao, X.; Fang, Q.; Zhu, J. Analyst 1998, 123, 1017-1021.

[11]. Vinas, P.; Lopez-Erroz, C.; Cerdan, E. J.; Campillo, N. Mikrochim. Acta 2000, 134, 83-87.

[12]. El-Gindy, A.; El-Yazby, F.; Mostafa, A.; Maher, M. M. J. Pharm. Biomed. Anal. 2004, 35(4), 703-713.

[13]. Tietz, N. W. Textbook of Clinical Chemistry, W. B. Saunders, Philadelphia, PA, 1986.

[14]. Costa-Neto C. O.; Pereira A. V.; Aniceto C.; Fatibello-Filho O. Talanta 1999, 48(3), 659-667.

[15]. Khan, M. A.; Jin, S. O.; Lee, S. H.; Chung, H. Y. Luminescence 2009, 24(2), 73-78.

[16]. Mohamed, A. M. I.; Mohamed, H. A.; Abdel-Latif, N. M.; Mohamed, M. R. J. AOAC Inter. 2011, 94(6), 1758-1769.

[17]. Szpikowska-Sroka, B. J. Anal. Chem. 2013, 68(3), 218-222.

[18]. Miller, J. C.; Miller, J. N. Statistics for Analytical Chemistry, 3rd ed. Horwood, Chichester, UK, 1993.

Supporting Agencies

Most read articles by the same author(s)

Dimensions - Altmetric - scite_ - PlumX

Downloads and views


Download data is not yet available.


Metrics Loading ...
License Terms

License Terms


Copyright © 2024 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License ( By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License ( are administered by Atlanta Publishing House LLC (European Journal of Chemistry).