OPEN ACCESS | PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS
Rapid chiral separation and impurity determination of ropivacaine and bupivacaine by Densitometry-HPTLC, using mucopolysaccharide as chiral mobile phase additive
Nahla Nour El-Din Salama (1,*)
(1) Pharmaceutical Chemistry, Department National Organization for Drug Control and Research, Giza, 35521, Egypt
(*) Corresponding Author
Received: 02 Nov 2013 | Revised: 27 Nov 2013 | Accepted: 28 Nov 2013 | Published: 30 Jun 2014 | Issue Date: June 2014
Enantio separation of the local anesthetic drugs; ropivacaine (Rop), bupivacaine (Bup) and potential organic impurities (2,6-Dimethylaniline, 2,6-DMA) were accomplished on HPTLC using mucopolysaccharide selector (Chondroitin) as chiral mobile phase additive (CMPA). The enantioseparation was achieved in acetonitrile:water:methanol (16:3:1, v:v:v) containing 0.25% chondroitin as chiral mobile phase additive. The influence of separation conditions, including type and concentration of chiral selector, organic modifiers and temperatures on enantioseparation were evaluated. The enantioselective HPTLC method was validated to control the enantiomeric purity of the (S)-enantiomers (S-Rop and S-Bup); the active ingredients contained in drug products. In these conditions, linearity over the concentration range, 1.0-10.0 µg/spot for each (R)-enantiomer and 1.0-8.0 µg/spot for 2,6-DMA main organic impurity were obtained. The detection limits are less than 0.6 µg/spot of chiral and organic impurities. The intra and inter-day assay precision was less than 3.0% (RSD%).
Our editors have decided to support scientists to publish their manuscripts in European Journal of Chemistry without any financial constraints.
1- The article processing fee will not be charged from the articles containing the single-crystal structure characterization between November 15, 2022 and December 28, 2022 (Voucher code: SINGLE2022).
2. Young writers will not be charged for the article processing fee between November 15, 2022 and December 28, 2022 (Voucher code: YOUNG2022).
3. The article processing fee will not be charged from the articles containing a part of the PhD thesis between November 15, 2022 and December 28, 2022 (Voucher code: PhD2022).
4. The article processing fee will not be charged from authors who have at least one publication in the European Journal of Chemistry between November 15, 2022 and December 28, 2022 (Voucher code: (Voucher code: AUTHOR2022).
European Journal of Chemistry
Links for Article
| | | | | | |
| | | | | | |
| | | |
Article MetricsThis Abstract was viewed 1283 times | PDF Article downloaded 541 times
. Zhijian Tan, Fenfang Li, Congcong Zhao, Yubin Teng, Yanfei Liu
Chiral separation of mandelic acid enantiomers using an aqueous two-phase system based on a thermo-sensitive polymer and dextran
Separation and Purification Technology 172, 382, 2017
. Shengqiang Tong, Hu Zhang, Jizhong Yan
Analytical Enantioseparation of β-Substituted-2-Phenylpropionic Acids by High-Performance Liquid Chromatography with Hydroxypropyl-β-Cyclodextrin as Chiral Mobile Phase Additive
Journal of Chromatographic Science 54(4), 593, 2016
. Wainer, I. W.; Editor, Drug Stereochemistry: Analytical Methods and Pharmacology, 2nd Ed., Marcel Dekker, New York, 1993.
. Albright, G. A. Anesthesiology 1979, 51, 285-287.
. Burlacu, C. L.; Buggy, D. J. Ther. Clin. Risk. Manag. 2008, 4, 381-392.
. McClure, J. H. Br. J. Anaesth. 1996, 76, 300-307.
. Valenzuela, C. V.; Snyders, D. J.; Bennett, P. B.; Tamargo, J.; Hondeghem, L. M. Circulation 1995, 92, 3014-3024.
. Tsuchiya, H.; Mizogami, M. Local Reg. Anesth. 2008, 1, 1-9.
. Amini, A.; Javerfalk, E.; Bastami, S.; Westerlund, D. Electrophoresis 1999, 20, 204-211.
. Javerfalk, E. M.; Amini, A.; Westerlund, D.; Andren, P. E. J. Mass Spectrom. 1998, 33, 183-186.
. Tang, W.; Muderawan, I. W.; Siu-Choon, N. G.; Chan, H. S. O. Anal. Chim. Acta 2006, 555, 63-67.
. Amini, A.; Wiersma, B.; Westerlund, D.; Paulsen-Sorman, U. Eur. J. Pharm. Sci. 1999, 9, 17-24.
. Fanali, S. J. Chromatogr. A 2000, 875, 89-122.
. Gubitz, G.; Schmid, M. G. Electrophoresis 2000, 21, 4112-4135.
. Nishi, H. J. Chromatogr. A 1997, 792, 327-347.
. Nystrom, A.; Strandberg, A.; Aspegren, A.; Behr, S.; Karlsson, A. Chromatographia 1999, 50, 208-219.
. Tanaka, Y.; Terabe, S. Chromatographia 1999, 49, 489-495.
. Schneiderman, E.; Stalcup, A. M. J. Chromatogr. B 2000, 745, 83-102.
. Arvidsson, T.; Bruce, H. F.; Halldin, M. M. Chirality 1995, 7, 272-277.
. Salama, N. N. J. Planar Chromatogr. Mod. TLC. 2008, 21, 441-446.
. Taha, E. A. Curr. Pharm. Chem. 2007, 3, 273-277.
. ICH Q2A. Validation of analytical methods definitions and terminology, in IFPMA (ed.), International Conference on Harmonization, Geneva 1994.
. ICH Q2B. Validation of analytical procedure methodology, in IFPMA (ed.), International Conference on Harmonization, Geneva 1996.
. Aboul-Enein, H. Y.; El-Awady, M. I.; Heard, C. H. J. Pharm. Biomed. Anal. 2003, 32, 1055-1059.
How to cite
The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item
DOI Link: https://doi.org/10.5155/eurjchem.5.2.214-218.964
| | | | | | | |
| | | | | | |
Save to Zotero Save to Mendeley
European Journal of Chemistry 2014, 5(2), 214-218 | doi: https://doi.org/10.5155/eurjchem.5.2.214-218.964 | Get rights and content
- There are currently no refbacks.
© Copyright 2010 - 2022 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2010-2022 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.