European Journal of Chemistry 2015, 6(2), 135-140 | doi: https://doi.org/10.5155/eurjchem.6.2.135-140.1178 | Get rights and content






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Spectrophotometric determination of ammonia using ninhydrin assay and kinetic studies


Hutaf Mustafa Baker (1,*) , Khairieh Fadi Alzboon (2)

(1) Department of Chemistry, Faculty of Science, Al Al-Bayt University, Mafraq, 25113, Jordan
(2) Department of Chemistry, Faculty of Science, Al Al-Bayt University, Mafraq, 25113, Jordan
(*) Corresponding Author

Received: 10 Nov 2014 | Accepted: 01 Feb 2015 | Published: 30 Jun 2015 | Issue Date: June 2015

Abstract


A spectrophotometric method for the determination of ammonia in aqueous media was investigated. This newly developed method for the reaction of ammonia with ninhydrin found to be simple, accurate and precise. A deep red color product was formed in the presence of sodium hydroxide at wavelength of 508 nm. The effects of variables such as temperature, time, acidity and reagents concentration have been evaluated to select the appropriate conditions for this reaction. Beer's law is obeyed; the calibration curve is linear over the range of 2×10-4-1.4×10-3 M of ammonia with regression coefficient (r2) of 0.994 (n = 7). The calculated molar absorptivity and Sandell's sensitivity values are 1.069×103 L/mol.cm and 0.015 μg/cm2, respectively. The limits of detection (LOD) and quantification (LOQ) are calculated to be 1.2×10-5 and 3.6×10-5 M, respectively. The intra-day accuracy expressed as relative error was less than 0.5% with precision (RSD) ranging from 0.49 to 1.55%. The inter-day accuracy ranged (RSD) from 0.49 to 1.90 % with a relative error was less than 1.50 %. The stoichiometry of this reaction was determined to be of 1:2 between ammonia and ninhydrin with first order reaction.


Keywords


Assay; Kinetic; Ammonia; Ninhydrin; Waste water; Spectrophotometric method

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DOI: 10.5155/eurjchem.6.2.135-140.1178

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Al Al-Bayt University, Mafraq, 25113, Jordan

Citations

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How to cite


Baker, H.; Alzboon, K. Eur. J. Chem. 2015, 6(2), 135-140. doi:10.5155/eurjchem.6.2.135-140.1178
Baker, H.; Alzboon, K. Spectrophotometric determination of ammonia using ninhydrin assay and kinetic studies. Eur. J. Chem. 2015, 6(2), 135-140. doi:10.5155/eurjchem.6.2.135-140.1178
Baker, H., & Alzboon, K. (2015). Spectrophotometric determination of ammonia using ninhydrin assay and kinetic studies. European Journal of Chemistry, 6(2), 135-140. doi:10.5155/eurjchem.6.2.135-140.1178
Baker, Hutaf, & Khairieh Fadi Alzboon. "Spectrophotometric determination of ammonia using ninhydrin assay and kinetic studies." European Journal of Chemistry [Online], 6.2 (2015): 135-140. Web. 25 Nov. 2020
Baker, Hutaf, AND Alzboon, Khairieh. "Spectrophotometric determination of ammonia using ninhydrin assay and kinetic studies" European Journal of Chemistry [Online], Volume 6 Number 2 (30 June 2015)

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DOI Link: https://doi.org/10.5155/eurjchem.6.2.135-140.1178

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