European Journal of Chemistry 2014, 5(3), 503-506. doi:10.5155/eurjchem.5.3.503-506.1070

Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus

Sayed Abdel Kader Ahmed (1) , Mahmoud Al-Refai (2,*)

(1) Department of Chemistry, Faculty of Science, Beni-Suef University, Beni-Suef, 62514, Egypt
(2) Department of Chemistry , Faculty of Science, Al Al-Bayt University, Mafraq, 25113, Jordan
(*) Corresponding Author

Received: 07 Apr 2014, Accepted: 13 May 2014, Published: 30 Sep 2014


The phytochemical investigation of the ethanolic extract of the aerial parts of Melilotus indicus resulted in the isolation of eight known flavonoids, namely; catechin (1), epicatechin (2), taxifolin (3), quercetin (4), quercetin 3-O-β-D-glucopyranoside (5), and quercetin 3-O-α-L-rhamnopyranoside (6), isorhamnetin 3-O-β-D-glucopyranoside (7) and isorhamnetin 3-O-β-D-rutinoside (8). These compounds have never been isolated from this species. The cytotoxic activities of the extracts of Melilotus indicus was evaluated by MTT assay against six cancer cell lines using doxorubicin as standard drug. The chloroform extract was the most potent cytotoxic agent against HepG-2cells with an IC50: 9.21 µg/mL.


Fabaceae; Phenolics; Flavonoids; Melilotus indicus; Cytotoxic activity; Phytochemical studies

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DOI: 10.5155/eurjchem.5.3.503-506.1070

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How to cite

Ahmed, S.; Al-Refai, M. Eur. J. Chem. 2014, 5(3), 503-506. doi:10.5155/eurjchem.5.3.503-506.1070
Ahmed, S.; Al-Refai, M. Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus. Eur. J. Chem. 2014, 5(3), 503-506. doi:10.5155/eurjchem.5.3.503-506.1070
Ahmed, S., & Al-Refai, M. (2014). Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus. European Journal of Chemistry, 5(3), 503-506. doi:10.5155/eurjchem.5.3.503-506.1070
Ahmed, Sayed, & Mahmoud Al-Refai. "Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus." European Journal of Chemistry [Online], 5.3 (2014): 503-506. Web. 17 Sep. 2019
Ahmed, Sayed, AND Al-Refai, Mahmoud. "Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus" European Journal of Chemistry [Online], Volume 5 Number 3 (30 September 2014)

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