European Journal of Chemistry 2014, 5(3), 503-506 | doi: https://doi.org/10.5155/eurjchem.5.3.503-506.1070 | Get rights and content

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Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus


Sayed Abdel Kader Ahmed (1) , Mahmoud Al-Refai (2,*)

(1) Department of Chemistry, Faculty of Science, Beni-Suef University, Beni-Suef, 62514, Egypt
(2) Department of Chemistry , Faculty of Science, Al Al-Bayt University, Mafraq, 25113, Jordan
(*) Corresponding Author

Received: 07 Apr 2014 | Revised: 10 May 2014 | Accepted: 13 May 2014 | Published: 30 Sep 2014 | Issue Date: September 2014

Abstract


The phytochemical investigation of the ethanolic extract of the aerial parts of Melilotus indicus resulted in the isolation of eight known flavonoids, namely; catechin (1), epicatechin (2), taxifolin (3), quercetin (4), quercetin 3-O-β-D-glucopyranoside (5), and quercetin 3-O-α-L-rhamnopyranoside (6), isorhamnetin 3-O-β-D-glucopyranoside (7) and isorhamnetin 3-O-β-D-rutinoside (8). These compounds have never been isolated from this species. The cytotoxic activities of the extracts of Melilotus indicus was evaluated by MTT assay against six cancer cell lines using doxorubicin as standard drug. The chloroform extract was the most potent cytotoxic agent against HepG-2cells with an IC50: 9.21 µg/mL.


Keywords


Fabaceae; Phenolics; Flavonoids; Melilotus indicus; Cytotoxic activity; Phytochemical studies

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DOI: 10.5155/eurjchem.5.3.503-506.1070

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[2]. Amer Ali Abd El-Hafeez, Hazim O. Khalifa, Rania Abdelrahman Elgawish, Samia A. Shouman, Magdy Hussein Abd El-Twab, Seiji Kawamoto
Melilotus indicus extract induces apoptosis in hepatocellular carcinoma cells via a mechanism involving mitochondria-mediated pathways
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DOI: 10.1007/s10616-018-0195-7
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[3]. Haroon Iftikhar, Dildar Ahmed, Muhammad T. Qamar
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[4]. Kyoung Jin Park, Joon Min Cha, Lalita Subedi, Sun Yeou Kim, Kang Ro Lee
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How to cite


Ahmed, S.; Al-Refai, M. Eur. J. Chem. 2014, 5(3), 503-506. doi:10.5155/eurjchem.5.3.503-506.1070
Ahmed, S.; Al-Refai, M. Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus. Eur. J. Chem. 2014, 5(3), 503-506. doi:10.5155/eurjchem.5.3.503-506.1070
Ahmed, S., & Al-Refai, M. (2014). Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus. European Journal of Chemistry, 5(3), 503-506. doi:10.5155/eurjchem.5.3.503-506.1070
Ahmed, Sayed, & Mahmoud Al-Refai. "Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus." European Journal of Chemistry [Online], 5.3 (2014): 503-506. Web. 12 Apr. 2021
Ahmed, Sayed, AND Al-Refai, Mahmoud. "Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus" European Journal of Chemistry [Online], Volume 5 Number 3 (30 September 2014)

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