European Journal of Chemistry 2015, 6(2), 135-140 | doi: https://doi.org/10.5155/eurjchem.6.2.135-140.1178 | Get rights and content

Issue cover





  Open Access OPEN ACCESS | Open Access PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS

Spectrophotometric determination of ammonia using ninhydrin assay and kinetic studies


Hutaf Mustafa Baker (1,*) , Khairieh Fadi Alzboon (2)

(1) Department of Chemistry, Faculty of Science, Al Al-Bayt University, Mafraq, 25113, Jordan
(2) Department of Chemistry, Faculty of Science, Al Al-Bayt University, Mafraq, 25113, Jordan
(*) Corresponding Author

Received: 10 Nov 2014 | Accepted: 01 Feb 2015 | Published: 30 Jun 2015 | Issue Date: June 2015

Abstract


A spectrophotometric method for the determination of ammonia in aqueous media was investigated. This newly developed method for the reaction of ammonia with ninhydrin found to be simple, accurate and precise. A deep red color product was formed in the presence of sodium hydroxide at wavelength of 508 nm. The effects of variables such as temperature, time, acidity and reagents concentration have been evaluated to select the appropriate conditions for this reaction. Beer's law is obeyed; the calibration curve is linear over the range of 2×10-4-1.4×10-3 M of ammonia with regression coefficient (r2) of 0.994 (n = 7). The calculated molar absorptivity and Sandell's sensitivity values are 1.069×103 L/mol.cm and 0.015 μg/cm2, respectively. The limits of detection (LOD) and quantification (LOQ) are calculated to be 1.2×10-5 and 3.6×10-5 M, respectively. The intra-day accuracy expressed as relative error was less than 0.5% with precision (RSD) ranging from 0.49 to 1.55%. The inter-day accuracy ranged (RSD) from 0.49 to 1.90 % with a relative error was less than 1.50 %. The stoichiometry of this reaction was determined to be of 1:2 between ammonia and ninhydrin with first order reaction.


Announcements


Our editors have decided to support scientists to publish their manuscripts in European Journal of Chemistry without any financial constraints.

1- The article processing fee will not be charged from the articles containing the single-crystal structure characterization between July 1, 2022 and August 15, 2022 (Voucher code: SINGLE2022).

2. Young writers will not be charged for the article processing fee between July 1, 2022 and August 15, 2022 (Voucher code: YOUNG2022).

3. The article processing fee will not be charged from the articles containing a part of the PhD thesis between July 1, 2022 and August 15, 2022 (Voucher code: PhD2022).

Editor-in-Chief

European Journal of Chemistry

Keywords


Assay; Kinetic; Ammonia; Ninhydrin; Waste water; Spectrophotometric method

Full Text:

PDF
PDF    Open Access

DOI: 10.5155/eurjchem.6.2.135-140.1178

Links for Article


| | | | | | |

| | | | | | |

| | | |

Related Articles




Article Metrics

icon graph This Abstract was viewed 1388 times | icon graph PDF Article downloaded 510 times

Funding information


Al Al-Bayt University, Mafraq, 25113, Jordan

Citations

/


[1]. Hutaf Mustafa Baker, Hussam Ahmad Alsaoud, Hamzeh Mohamad Abdel-Halim
Spectrophotometric method for determination of ranitidine hydrochloride in bulk and in pharmaceutical preparation using ninhydrin
European Journal of Chemistry  11(4), 291, 2020
DOI: 10.5155/eurjchem.11.4.291-297.2002
/


[2]. Md Mahamudul Hasan Mredul, Antara Binta Akkas, Nagham Elsaidy, Mohammad Lokman Ali, Md. Anwar Hossain Mondal, Md Rushna Alam
Using of mustard oil cake in safe organic aquaculture through increasing pond primary productivity
Aquaculture Reports  23, 101073, 2022
DOI: 10.1016/j.aqrep.2022.101073
/


[3]. Pirom Didpinrum, Watsaka Siriangkhawut, Kraingkrai Ponhong, Piyanete Chantiratikul, Kate Grudpan
A newly designed sticker-plastic sheet platform and smartphone-based digital imaging for protein assay in food samples with downscaling Kjeldahl digestion
RSC Advances  11(58), 36494, 2021
DOI: 10.1039/D1RA04321H
/


[4]. Ibrahim A. Salem, Mohamed H. Shaltout, Ahmed B. Zaki
Homogeneous and heterogeneous catalytic oxidation of some azo dyes using copper(II) ions
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy  227, 117618, 2020
DOI: 10.1016/j.saa.2019.117618
/


[5]. Abhijeet Rana, Soutick Nandi, Shyam Biswas
Sulfonic acid functionalized zirconium-based metal–organic framework for the selective detection of copper(ii) ions
New Journal of Chemistry  46(21), 10477, 2022
DOI: 10.1039/D2NJ01068B
/


References


[1]. Qian, P. L.; Jia-Zhong, Z.; Frank, J.; Dennis, M.; Hansell, A. Mar. Chem. 2005, 6, 73-85.

[2]. Michalski, R.; Kurzyca, I. Pol. J. Environ. Stud. 2006, 15(1), 5-18.

[3]. Skjemstad, J. O.; Reeve, R. J. Environ. Qual. 1976, 7, 137-141.
http://dx.doi.org/10.2134/jeq1978.00472425000700010028x

[4]. Marczenko, Z. Separation and spectrophotometric determination of elements, 4th Edit., John Wiley and Sons, 1986.

[5]. Svehla, G. Pure Appl. Chem. 1993, 65(10), 2291-2298.
http://dx.doi.org/10.1351/pac199365102291

[6]. Al-Tamimi, S. A.; Aly, F. A.; Almutairi, A. M. J. Anal. Chem. 2013, 68(4), 313-320.
http://dx.doi.org/10.1134/S1061934813040102

[7]. Rahman, N.; Rahman, H.; Azmi, N. H. Int. J. Bio. Life Sci. 2006, 2(1), 52-58.

[8]. Kalynaramu, B.; Raghubabu, K. Int. J. Chem. Tech. Res. 2011, 3, 1279-1284.

[9]. Basavaiah, K.; Tharpa, K.; Hiriyanna, S. G.; Vinay, K. B. J. Food. Drug. Anal. 2009, 17, 93-99.

[10]. Sutormina, E. F. J. Anal. Chem+ 2004, 59, 331-334.
http://dx.doi.org/10.1023/B:JANC.0000022784.32604.a8

[11]. Mahmood, K.; F. Wattoo, H.; Wattoo, M. H. S.; Imran, M.; Asad, M. J.; Tirmizi, S. A.; Wadood, A. Saudi. J. Bio. Sci. 2012, 19, 247-250.
http://dx.doi.org/10.1016/j.sjbs.2012.01.001

[12]. Jeong, H.; Park, J.; Kim, H. Y. J. Chem. 2013, 2013, 1-9.

[13]. Sekran, B.; Rani, P. J. Pharm. Pharm. Sci. 2010, 2, 138-142.

[14]. Salem, H. Chin. Pharm. J. 1999, 51, 123-142.

[15]. El-Enany, N.; Belal, F.; Rizk M. Sci. Pharm. 2006, 74, 99-119.
http://dx.doi.org/10.3797/scipharm.2006.74.99

[16]. Kukoc-Modun, L.; Radic, N. Anal. Sci. 2010, 26, 491-495.
http://dx.doi.org/10.2116/analsci.26.491

[17]. Mohamed, M. A.; Abdellatef, H. E.; Hosny, M. M.; Sharaf, A. Y. Eur. J. Chem. 2013, 4(1), 35‐43.
http://dx.doi.org/10.5155/eurjchem.4.1.35-43.713

[18]. Rahman, N.; Siddiqui M. R.; Azmi, S. N. H. J. Chin. Chem. Soc. 2006, 53, 735-743.
http://dx.doi.org/10.1002/jccs.200600097

[19]. Christian, G. D. Data Handling, Analytical Chemistry, 6th Edition, John Wiley and Sons, 2004.

[20]. Abdellatef, H. E.; Ayad, M. M.; Taha E. A. J. Pharmaceut. Biomed. 1999, 18, 1021-1027.
http://dx.doi.org/10.1016/S0731-7085(98)00113-7


How to cite


Baker, H.; Alzboon, K. Eur. J. Chem. 2015, 6(2), 135-140. doi:10.5155/eurjchem.6.2.135-140.1178
Baker, H.; Alzboon, K. Spectrophotometric determination of ammonia using ninhydrin assay and kinetic studies. Eur. J. Chem. 2015, 6(2), 135-140. doi:10.5155/eurjchem.6.2.135-140.1178
Baker, H., & Alzboon, K. (2015). Spectrophotometric determination of ammonia using ninhydrin assay and kinetic studies. European Journal of Chemistry, 6(2), 135-140. doi:10.5155/eurjchem.6.2.135-140.1178
Baker, Hutaf, & Khairieh Fadi Alzboon. "Spectrophotometric determination of ammonia using ninhydrin assay and kinetic studies." European Journal of Chemistry [Online], 6.2 (2015): 135-140. Web. 16 Aug. 2022
Baker, Hutaf, AND Alzboon, Khairieh. "Spectrophotometric determination of ammonia using ninhydrin assay and kinetic studies" European Journal of Chemistry [Online], Volume 6 Number 2 (30 June 2015)

The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item



DOI Link: https://doi.org/10.5155/eurjchem.6.2.135-140.1178

CrossRef | Scilit | GrowKudos | Researchgate | Publons | ScienceGate | scibey | Scite | Lens | OUCI

WorldCat Paperbuzz | LibKey Citeas | Dimensions | Semanticscholar | Plumx | Kopernio | Zotero | Mendeley

ZoteroSave to Zotero MendeleySave to Mendeley



European Journal of Chemistry 2015, 6(2), 135-140 | doi: https://doi.org/10.5155/eurjchem.6.2.135-140.1178 | Get rights and content

Refbacks

  • There are currently no refbacks.




Copyright (c)





© Copyright 2010 - 2022  Atlanta Publishing House LLC All Right Reserved.

The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.

Copyright 2010-2022 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.