European Journal of Chemistry

Voltammetric and viscometric studies of flavonoids interactions with DNA at physiological conditions



Main Article Content

Syed Tajammul Hussain
Asima Siddiqa

Abstract

Cyclic voltammetric behaviour of three flavonoids: quercetin, morin and rutin was investigated for their interaction with DNA at pH = 4.7 and pH = 7.4 at body temperature (310 K), using glassy carbon electrode. The diffusion coefficients of the free and DNA bound forms of the flavonoids were evaluated using Randles–Sevcik equation. The binding parameters like binding constant, binding site size and binding free energy were also determined from voltammetric data. Moreover, the binding modes of flavonoids with DNA were evaluated from viscommetric analysis. A comparatively high value of binding constant, binding site size and diffusion coefficients for quercetin reveals its stronger binding with DNA under physiological conditions. The negative values of ∆G indicate the spontaneity of binding of flavonoids with DNA.

2_1_109_112_800


icon graph This Abstract was viewed 1601 times | icon graph Article PDF downloaded 671 times

How to Cite
(1)
Hussain, S. T.; Siddiqa, A. Voltammetric and Viscometric Studies of Flavonoids Interactions With DNA at Physiological Conditions. Eur. J. Chem. 2011, 2, 109-112.

Article Details

Share
Crossref - Scopus - Google - European PMC
References

[1]. Battin, E. E.; Perron, N. R.; Brumaghim, J. L. Inorg. Chem. 2006, 45, 499-501.
doi:10.1021/ic051594f
PMid:16411681

[2]. Ader, P.; Wessmann, A.; Wolffram, S. Free Rad. Biol. and Med. 2000, 28, 1056-1060.
doi:10.1016/S0891-5849(00)00195-7

[3]. Russo, A.; Acquaviva, R.; Campisi, A. Cell Biol. Toxicol. 2000, 16, 91-95.
doi:10.1023/A:1007685909018
PMid:10917564

[4]. Ren, W.; Qiao, Z.; Wang, H.; Zhu, L.; Zhang, L. Med. Res. Rev. 2003, 23, 519-523.
doi:10.1002/med.10033
PMid:12710022

[5]. Middleton, E.; Kandaswami, C.; Theoharides T. C. Pharmacol. Rev. 2000, 52, 673-680.
PMid:11121513

[6]. Pietta, P. G. J. Nat. Prod. 2000, 63, 1035-1039.
doi:10.1021/np9904509
PMid:10924197

[7]. Hollman, P. C.; Katan, M. B. Food Chem. Toxicol. 1999, 37, 937-941.
doi:10.1016/S0278-6915(99)00079-4

[8]. Vijayalakshmi, R.; Kantimathi, M.; Subramanian, V. Biochem. Biophys. Res. Commun. 2000, 271, 731-735.
doi:10.1006/bbrc.2000.2707
PMid:10814531

[9]. Shah, A.; Qureshi, R.; Kausar, N.; Haque, S. Anal. Sci. 2008, 24, 1437-1441.
doi:10.2116/analsci.24.1437
PMid:18997372

[10]. Zhu, Z.; Li, C.; Li, N. Q. Microchem. J. 2002, 71, 57-62.
doi:10.1016/S0026-265X(01)00118-7

[11]. Cao, Y.; He, X. Spectrochim. Acta A 1998, 54, 883-884.
doi:10.1016/S1386-1425(97)00277-1

[12]. Wang, F.; Xu, Y.; Zhao, J.; Shengshui, H. Bioelectrochem. 2007, 70, 356-361.
doi:10.1016/j.bioelechem.2006.05.003
PMid:16787762

[13]. Kanakis, C. D.; Tarantilis, P. A.; Polissou, M. S.; Diamantoglou, S. J. Biomol. Struc. Dyn. 2005, 22, 739-742.

[14]. Kang, J.; Li, Z.; Lu, X. J. Pharm. Biomed. Anal. 2006, 40, 1166-1170.
doi:10.1016/j.jpba.2005.08.030
PMid:16256288

[15]. Aslanoglu, M. Anal. Sci. 2006, 22, 439-445.
doi:10.2116/analsci.22.439
PMid:16733319

[16]. Janjua, N. K.; Siddiqa, A.; Yaqub, A.; Sabahat, S.; Qureshi, R.; Haque, S. Spectrochim. Acta. A 2009, 74, 1135-1140.
doi:10.1016/j.saa.2009.09.022
PMid:19836298

[17]. Aslanoglu, M. Turk. J. Chem. 2005, 29, 477-481.

[18]. Veal, J. M.; Rill, R. L. Biochem. 1991, 30, 1132-1136.
doi:10.1021/bi00218a035
PMid:1989681

Supporting Agencies

Quaid-i-Azam University
TrendMD

Dimensions - Altmetric - scite_ - PlumX

Downloads and views

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...
License Terms

License Terms

by-nc

Copyright © 2024 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at https://www.eurjchem.com/index.php/eurjchem/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (https://www.eurjchem.com/index.php/eurjchem/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).