European Journal of Chemistry 2017, 8(1), 8-12 | doi: https://doi.org/10.5155/eurjchem.8.1.8-12.1514 | Get rights and content

Issue cover





  Open Access OPEN ACCESS | Open Access PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS

Validated simultaneous spectrophotometric quantification of a new antiviral combination


Sherif Abdel-Naby Abdel-Gawad (1,*)

(1) Pharmaceutical Chemistry Department, College of Pharmacy, Prince Sattam Bin-Abdul Aziz University, Al-Kharj, 11942, Kingdom of Saudi Arabia
(*) Corresponding Author

Received: 07 Dec 2016 | Revised: 05 Jan 2017 | Accepted: 07 Jan 2017 | Published: 31 Mar 2017 | Issue Date: March 2017

Abstract


Simple, selective and precise spectrophotometric methods were applied for simultaneous quantification of sofosbuvir (SFV) and ledipasvir (LDI) both in their raw and dosage forms. In the first method, the two cited drugs were determined simultaneously using first derivative (D1) method. It was accomplished by measuring peak heights at 275 nm and 344 nm, for SFV and LDI, respectively, in concentration ranges of 5 - 80 μg/mL and 3 - 50 μg/mL, for SFV and LDI, respectively. In the second one, a first derivative of ratio spectra (1DD) method was adopted to quantify SFV in concentration range of 5 - 80 µg/mL. It was adopted by measuring the peak amplitudes (valley and peak) at 259 nm and 280 nm, using 25 µg/mL LDI as a divisor. The proposed method was also used to determine LDI in concentration range of 3 - 50 µg/mL by recording the peak amplitudes (valley and peak) at 319 nm and 375 nm, using 80 µg/mL SFV as a divisor. The developed methods were validated with respect to linearity, accuracy, precision, selectivity, robustness and limits of detection and quantification (LOD and LOQ), as per the guidelines of International Conference on Harmonization (ICH)-Q2B.


Keywords


Ledipasvir; Sofosbuvir; Dosage form; Antiviral combination; Spectrophotometric method; Derivative spectrophotometry

Full Text:

PDF
PDF    Open Access

DOI: 10.5155/eurjchem.8.1.8-12.1514

Links for Article


| | | | | | |

| | | | | | |

| | |

Related Articles




Article Metrics

icon graph This Abstract was viewed 773 times | icon graph PDF Article downloaded 303 times

Funding information


The Central Laboratory, College of Science, Prince Sattam Bin Abdul-Aziz University, Kingdom of Saudi Arabia

Citations

/


[1]. Fatma A.M. Abdel-aal, Azza H. Rageh, Mohamed I. Said, Gamal A. Saleh
ε-MnO2-modified graphite electrode as a novel electrochemical sensor for the ultrasensitive detection of the newly FDA approved Hepatitis C antiviral drug ledipasvir
Analytica Chimica Acta  1038, 29, 2018
DOI: 10.1016/j.aca.2018.07.018
/


[2]. Mohamed M. El-Wekil, Ashraf M. Mahmoud, Saad A. Alkahtani, Adel A. Marzouk, Ramadan Ali
A facile synthesis of 3D NiFe2O4 nanospheres anchored on a novel ionic liquid modified reduced graphene oxide for electrochemical sensing of ledipasvir: Application to human pharmacokinetic study
Biosensors and Bioelectronics  109, 164, 2018
DOI: 10.1016/j.bios.2018.03.015
/


[3]. Hanaa S. El-Desoky, Mohamed M. Ghoneim, Mohamed A. Momtaz, Mohamed M. Abdel-Galeil
Utilizing the Synergetic Benefit of Synthesized NiO Nano Flakes and Natural Activated Charcoal (an Alternative of Graphene) as a Novel Platform for Voltammetric Determination of Chronic Hepatitis C Virus Inhibitor Ledipasvir
Journal of The Electrochemical Society  167(11), 117504, 2020
DOI: 10.1149/1945-7111/ab9e86
/


[4]. Mansuri Reema, Patel Dhara, Patel Khushboo, Meshram Dhananjay
In depth investigation of quantitative analytical and bioanalytical techniques of hepatitic drug sofosbuvir in different matrices: a review
Journal of Analytical & Pharmaceutical Research  7(2), , 2018
DOI: 10.15406/japlr.2018.07.00228
/


References


[1]. Te, H. S.; Jensen, D. M. Clin. Liver Dis. 2010, 14, 1-21.
https://doi.org/10.1016/j.cld.2009.11.009

[2]. Gane, E. J.; Stedman, C. A.; Hyland, R. H.; Ding, X.; Svarovskaia, E.; Symonds, W. T.; Hindes, R. G.; Berrey, M. M. New Engl. J. Med. 2013, 368, 34-44.
https://doi.org/10.1056/NEJMoa1208953

[3]. Gentile, I.; Buonomo, A. R.; Borgia, F.; Castaldo, G.; Borgia, G. Expert Open. Inv. Drug. 2014, 23, 561-571.

[4]. Afdhal, N.; Zeuzem, S.; Kwo, P. Chojkier, M.; Gitlin, N.; Puoti, M.; Romero-Gomez, M.; Zarski, J. -P.; Agarwal, K.; Buggisch, P. New Engl. J. Med. 2014, 370, 1889-1898.
https://doi.org/10.1056/NEJMoa1402454

[5]. Bahrami, M. T.; Mohammadi, B.; Miraghaei, S.; Babaei, A.; Ghaheri, M.; Bahrami, G. J. Sep. Sci. 2016, 39, 2702-2709.
https://doi.org/10.1002/jssc.201501375

[6]. Abdel-Gawad, S. A. Eur. J. Chem. 2016, 7, 375-379.
https://doi.org/10.5155/eurjchem.7.3.375-379.1439

[7]. Nebsen, M.; Elzanfaly, E. S. J. Chromatogr. Sci. 2016, 54, 1631-1640.
https://doi.org/10.1093/chromsci/bmw119

[8]. Devilal, J.; Durgaprasad, B.; Pal, N.; Rao, A. S. World J. Pharm. Pharm. Sci. 2016, 5, 1312-1321.

[9]. Zaman, B.; Siddique, F.; Hassan, W. Chromatographia 2016, 79, 1605-1613.
https://doi.org/10.1007/s10337-016-3179-9

[10]. Pan, C.; Chen, Y.; Chen, W.; Zhou, G.; Jin, L.; Zheng, Y.; Lin, W.; Pan, Z. J. Chromatogr. B 2016, 1008, 255-259.
https://doi.org/10.1016/j.jchromb.2015.11.056

[11]. Ariaudo, A.; Favata, F.; De Nicolo, A.; Simiele, M.; Paglietti, L.; Boglione, L.; Cardellino, C. S.; Carcieri, C.; Di Perri, G.; D'Avolio, A. J. Pharmaceut. Biomed. 2016, 125, 369-375.
https://doi.org/10.1016/j.jpba.2016.04.031

[12]. Elkady, E. F.; Aboelwafa, A. A. J. AOAC Int. 2016, 99, 1252-1259.
https://doi.org/10.5740/jaoacint.16-0021

[13]. Rezk, M. R.; Bendas, E. R.; Basalious, E. B.; Karim, I. A. J. Chromatogr. B 2016, 1028, 63-70.
https://doi.org/10.1016/j.jchromb.2016.06.004

[14]. ICH harmonized tripartite guideline, validation of analytical procedures: Text and methodology Q2 (B), 2005.

[15]. Abdel-Gawad, S. A.; Elsherif, Z. A. Eur. J. Chem. 2012, 3, 447-454.
https://doi.org/10.5155/eurjchem.3.4.447-454.681

[16]. Slainas, F.; Nevado, J. J.; Mansilla, A. E. Talanta 1990, 37, 347-351.
https://doi.org/10.1016/0039-9140(90)80065-N

[17]. Darwish, H. W.; Hassan, S. A.; Salem, M. Y.; El-Zeiny, B. A. Spectrochim. Acta A 2011, 83, 140-148.
https://doi.org/10.1016/j.saa.2011.08.005


How to cite


Abdel-Gawad, S. Eur. J. Chem. 2017, 8(1), 8-12. doi:10.5155/eurjchem.8.1.8-12.1514
Abdel-Gawad, S. Validated simultaneous spectrophotometric quantification of a new antiviral combination. Eur. J. Chem. 2017, 8(1), 8-12. doi:10.5155/eurjchem.8.1.8-12.1514
Abdel-Gawad, S. (2017). Validated simultaneous spectrophotometric quantification of a new antiviral combination. European Journal of Chemistry, 8(1), 8-12. doi:10.5155/eurjchem.8.1.8-12.1514
Abdel-Gawad, Sherif. "Validated simultaneous spectrophotometric quantification of a new antiviral combination." European Journal of Chemistry [Online], 8.1 (2017): 8-12. Web. 2 Mar. 2021
Abdel-Gawad, Sherif. "Validated simultaneous spectrophotometric quantification of a new antiviral combination" European Journal of Chemistry [Online], Volume 8 Number 1 (31 March 2017)

The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item



DOI Link: https://doi.org/10.5155/eurjchem.8.1.8-12.1514

CrossRef | Scilit | GrowKudos | Researchgate | Publons | Microsoft | scibey | Scite | Lens | OUCI

WorldCat Paperbuzz Citeas | Dimensions | Semanticscholar | Plumx | Kopernio | Zotero | Mendeley

ZoteroSave to Zotero MendeleySave to Mendeley



European Journal of Chemistry 2017, 8(1), 8-12 | doi: https://doi.org/10.5155/eurjchem.8.1.8-12.1514 | Get rights and content

Refbacks

  • There are currently no refbacks.




Copyright (c)





© Copyright 2010 - 2021  Atlanta Publishing House LLC All Right Reserved.

The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.

Copyright 2010-2021 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.