European Journal of Chemistry 2017, 8(1), 8-12. doi:10.5155/eurjchem.8.1.8-12.1514

Validated simultaneous spectrophotometric quantification of a new antiviral combination


Sherif Abdel-Naby Abdel-Gawad (1,*)

(1) Pharmaceutical Chemistry Department, College of Pharmacy, Prince Sattam Bin-Abdul Aziz University, Al-Kharj, 11942, Kingdom of Saudi Arabia
(*) Corresponding Author

Received: 07 Dec 2016, Accepted: 07 Jan 2017, Published: 31 Mar 2017

Abstract


Simple, selective and precise spectrophotometric methods were applied for simultaneous quantification of sofosbuvir (SFV) and ledipasvir (LDI) both in their raw and dosage forms. In the first method, the two cited drugs were determined simultaneously using first derivative (D1) method. It was accomplished by measuring peak heights at 275 nm and 344 nm, for SFV and LDI, respectively, in concentration ranges of 5 - 80 μg/mL and 3 - 50 μg/mL, for SFV and LDI, respectively. In the second one, a first derivative of ratio spectra (1DD) method was adopted to quantify SFV in concentration range of 5 - 80 µg/mL. It was adopted by measuring the peak amplitudes (valley and peak) at 259 nm and 280 nm, using 25 µg/mL LDI as a divisor. The proposed method was also used to determine LDI in concentration range of 3 - 50 µg/mL by recording the peak amplitudes (valley and peak) at 319 nm and 375 nm, using 80 µg/mL SFV as a divisor. The developed methods were validated with respect to linearity, accuracy, precision, selectivity, robustness and limits of detection and quantification (LOD and LOQ), as per the guidelines of International Conference on Harmonization (ICH)-Q2B.


Keywords


Ledipasvir; Sofosbuvir; Dosage form; Antiviral combination; Spectrophotometric method; Derivative spectrophotometry

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DOI: 10.5155/eurjchem.8.1.8-12.1514

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Citations

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[1]. Fatma A.M. Abdel-aal, Azza H. Rageh, Mohamed I. Said, Gamal A. Saleh
ε-MnO2-modified graphite electrode as a novel electrochemical sensor for the ultrasensitive detection of the newly FDA approved Hepatitis C antiviral drug ledipasvir
Analytica Chimica Acta  1038, 29, 2018
DOI: 10.1016/j.aca.2018.07.018
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[2]. Mohamed M. El-Wekil, Ashraf M. Mahmoud, Saad A. Alkahtani, Adel A. Marzouk, Ramadan Ali
A facile synthesis of 3D NiFe2O4 nanospheres anchored on a novel ionic liquid modified reduced graphene oxide for electrochemical sensing of ledipasvir: Application to human pharmacokinetic study
Biosensors and Bioelectronics  109, 164, 2018
DOI: 10.1016/j.bios.2018.03.015
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[3]. Mansuri Reema, Patel Dhara, Patel Khushboo, Meshram Dhananjay
In depth investigation of quantitative analytical and bioanalytical techniques of hepatitic drug sofosbuvir in different matrices: a review
Journal of Analytical & Pharmaceutical Research  7(2), , 2018
DOI: 10.15406/japlr.2018.07.00228
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How to cite


Abdel-Gawad, S. Eur. J. Chem. 2017, 8(1), 8-12. doi:10.5155/eurjchem.8.1.8-12.1514
Abdel-Gawad, S. Validated simultaneous spectrophotometric quantification of a new antiviral combination. Eur. J. Chem. 2017, 8(1), 8-12. doi:10.5155/eurjchem.8.1.8-12.1514
Abdel-Gawad, S. (2017). Validated simultaneous spectrophotometric quantification of a new antiviral combination. European Journal of Chemistry, 8(1), 8-12. doi:10.5155/eurjchem.8.1.8-12.1514
Abdel-Gawad, Sherif. "Validated simultaneous spectrophotometric quantification of a new antiviral combination." European Journal of Chemistry [Online], 8.1 (2017): 8-12. Web. 23 Oct. 2019
Abdel-Gawad, Sherif. "Validated simultaneous spectrophotometric quantification of a new antiviral combination" European Journal of Chemistry [Online], Volume 8 Number 1 (31 March 2017)

DOI Link: https://doi.org/10.5155/eurjchem.8.1.8-12.1514

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