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New HPLC method for determination of cystatin C biomarker in human blood
Othman Ibrahem AlMusaimi (1,*)
, Abd-Alhakeem Hasan Abu-Nawwas (2) , Danah Mahdi AlShaer (3) , Nabeel Yousef Khaleel (4) (1) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(2) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(3) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(4) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(*) Corresponding Author
Received: 04 Oct 2017 | Revised: 24 Oct 2017 | Accepted: 25 Oct 2017 | Published: 31 Dec 2017 | Issue Date: December 2017
Abstract
A rapid and sensitive method for determination of cystatin C (CC) protein in human blood by means of high performance liquid chromatography (HPLC) was developed and validated. Estimated glomerular filtration rate (eGFR) has been calculated for the patients with chronic kidney disease (CKD). Ion pair liquid chromatography technique was utilized to separate CC along with UV detection. Calibration curve with excellent correlation (r2 = 0.99) over the range from 0.75 to 20.50 mg/L of CC was accomplished. Limits of detection and quantify-cation were 0.375 and 0.75 mg/L of CC, respectively. The recoveries were in the range of 93.6-102.3%. Intra-assay and inter-assay variabilities were 8.2 and 6.14%, respectively.
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DOI: 10.5155/eurjchem.8.4.378-383.1658
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Funding information
King Abdul Aziz City for Science and Technology (KACST): Grant# SP-36-16.
Citations
[1]. O. Al Musaimi, A.H. Abu-Nawwas, D. Al Shaer, N.Y. Khaleel, M. Fawzi
Influence of age, gender, smoking, diabetes, thyroid and cardiac dysfunctions on cystatin C biomarker
Medicina de Familia. SEMERGEN 45(1), 44, 2019
DOI: 10.1016/j.semerg.2018.07.005
[2]. Wenying Zhao, Zhiqiang Wu, Zhixian Fan, Shuguang Xiang
Determination of formic, acetic, propionic, and n-butyric acids by indirect photometric chromatography
Journal of Liquid Chromatography & Related Technologies 41(9), 481, 2018
DOI: 10.1080/10826076.2018.1465434
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DOI Link: https://doi.org/10.5155/eurjchem.8.4.378-383.1658
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European Journal of Chemistry 2017, 8(4), 378-383 | doi: https://doi.org/10.5155/eurjchem.8.4.378-383.1658 | Get rights and content
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