European Journal of Chemistry

New HPLC method for determination of cystatin C biomarker in human blood

Article Sidebar

PDF
Published: Dec 31, 2017
DOI: https://doi.org/10.5155/eurjchem.8.4.378-383.1658
Keywords:
Cystatin C, Biomarker, Kidney failure, Chromatography, Glomerular filtration rate, Determination of cystatin C
Section
Research Article
Issue
Vol. 8 No. 4 (2017): December 2017
Dates
Received 2017-10-04
Accepted 2017-10-25
Published 2017-12-31


Main Article Content

Othman Ibrahem AlMusaimi
Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
Abd-Alhakeem Hasan Abu-Nawwas
Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
Danah Mahdi AlShaer
Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
Nabeel Yousef Khaleel
Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia

Abstract

A rapid and sensitive method for determination of cystatin C (CC) protein in human blood by means of high performance liquid chromatography (HPLC) was developed and validated. Estimated glomerular filtration rate (eGFR) has been calculated for the patients with chronic kidney disease (CKD). Ion pair liquid chromatography technique was utilized to separate CC along with UV detection. Calibration curve with excellent correlation (r2 = 0.99) over the range from 0.75 to 20.50 mg/L of CC was accomplished. Limits of detection and quantify-cation were 0.375 and 0.75 mg/L of CC, respectively. The recoveries were in the range of 93.6-102.3%. Intra-assay and inter-assay variabilities were 8.2 and 6.14%, respectively.


icon graph This Abstract was viewed 2610 times | icon graph Article PDF downloaded 861 times

How to Cite
(1)
AlMusaimi, O. I.; Abu-Nawwas, A.-A. H.; AlShaer, D. M.; Khaleel, N. Y. New HPLC Method for Determination of Cystatin C Biomarker in Human Blood. Eur. J. Chem. 2017, 8, 378-383.

Article Details

Share
Links for Article


Crossref - Scopus - Google - European PMC
Crossref
Scopus
Google Scholar
Europe PMC
References

[1]. Marshall, W. J.; Lapsley, M.; Day, A. Clinical Chemistry, 8th ed., Chapter 5, pp. 73-97, Edinburgh, Elsevier, 2016.

[2]. Mussap, M.; Plebani, M. Crit. Rev. Clin. Lab. Sci. 2004, 41(5-6), 467-550.
https://doi.org/10.1080/10408360490504934

[3]. Paskalev, E.; Lambreva, L.; Simeonov, P.; Koicheva, N.; Beleva, B.; Genova, M.; Marcovska, R.; Nashkov, A. Clin. Chim. Acta 2001, 310, 53-56.
https://doi.org/10.1016/S0009-8981(01)00522-8

[4]. Uzun, H.; Keles, M.; Ataman, R.; Aydin, S.; Kalender, U. E.; Simsek, G.; Halac, M.; Kaya, S. Clin. Biochem. 2005, 38, 792-798.
https://doi.org/10.1016/j.clinbiochem.2005.05.012

[5]. Finney, H.; Bates, C. J.; Price, C. P. Arch. Gerontol. Geriatr. 1999, 29, 75-94.
https://doi.org/10.1016/S0167-4943(99)00025-4

[6]. Hoek, F. J.; Kemperman, F. A. W.; Krediet, R. T. Nephrol. Dial. Transplant. 2003, 18, 2024-2031.
https://doi.org/10.1093/ndt/gfg349

[7]. Demirtas, S.; Akan, O.; Can, M.; Elmali, E.; Akan, H. Clin. Biochem. 2006, 39, 115-118.
https://doi.org/10.1016/j.clinbiochem.2005.10.009

[8]. Filler, G.; Bokenkamp, A.; Hofmann, W.; LeBricon, T.; Martinez-Bru, C.; Grubb, A. Clin. Biochem. 2005, 38, 1-8.
https://doi.org/10.1016/j.clinbiochem.2004.09.025

[9]. Bobek, L. A.; Levine, M. J. Crit. Rev. Oral Biol. Med. 1992, 3(4), 307-332.
https://doi.org/10.1177/10454411920030040101

[10]. Uchida, K.; Gotoh, A. Clin. Chim. Acta 2002, 323, 121-128.
https://doi.org/10.1016/S0009-8981(02)00177-8

[11]. Finney, H.; Newman, D. J.; Gruber, W.; Merle, P.; Price, C. P. Clin Chem. 1997, 43(6), 1016-1022.

[12]. Laterza, O. F.; Price, C. P.; Scott, M. G. Clin. Chem. 2002, 48(5), 699-707.

[13]. Kyhse-Anderson, J.; Schmidt, C.; Nordin, G.; Anderson, B.; Ehle, P. N.; Lindstrom, V.; Grubb, A. Clin Chem. 1994, 40(10), 1921-1926.

[14]. Mussap, M.; Vestra, M. D.; Fioretto, P.; Saller, A.; Varagnolo, M.; Nosadini, R.; Plebani, M. Kidney Int. 2002, 61(4), 1453-1461.
https://doi.org/10.1046/j.1523-1755.2002.00253.x

[15]. Storme, M. L.; Sinnaeve, B. A.; VanBocxlaer, J. F. J. Sep. Sci. 2005, 28, 1759-1763.
https://doi.org/10.1002/jssc.200500127

[16]. Huoyan, J.; Jianxin, W.; Shaoqin, J.; Hui, C.; Xudong, W.; Jianyou, S.; Huimin, W. J. Chromatogr. B 2017, 1059, 49-55.
https://doi.org/10.1016/j.jchromb.2017.04.007

[17]. Al-Musaimi, O.; Fayyad, M. K.; Mishal, A. K. J. Chromatogr. B 2009, 877, 747-750.
https://doi.org/10.1016/j.jchromb.2009.02.009

[18]. Al-Musaimi, O.; Fayyad, M. K.; Mishal, A. K. Cystatins: protease inhibitors, biomarkers, and immunomodulators, Ch. 10. Nova Publisher, pp. 215-224, 2010.

[19]. ICH Harmonised Tripartite Guideline - Validation of analytical procedures: text and methodology Q2(R1). 2005 [Accessed on October 26, 2017]; Available from: http://www.ich.org/fileadmin/ Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf.

[20]. Draft guidance for industry - Bioanalytical method validation. 2013 [Accessed on October 26, 2017]; Available from: https:// www.fda.gov/downloads/Drugs/Guidances/ucm368107.pdf .

[21]. Gerhartz, B.; Ekiel, I.; Abrahamson, M. Biochemistry 1998, 37, 17309-17317.
https://doi.org/10.1021/bi980873u

[22]. Cao, E.; Chen, Y.; Cui, Z.; Foster, P. R. Biotechnol. Bioeng. 2002, 82, 684-690.
https://doi.org/10.1002/bit.10612

[23]. Spithoven, E. M.; Bakker, S. J.; Kootstra-Ros, J. E; Jong, P. E.; Gansevoort, R. T. Clin. Biochem. 2013, 46(15), 1611-1614.
https://doi.org/10.1016/j.clinbiochem.2013.06.022

[24]. Knight, E. L.; Verhave, J. C.; Spiegelman, D.; Hillege, H. L.; Zeeuw, D.; Curhan, G. C.; Jong, P. E. Kidney Int. 2004, 65(4), 1416-1421.
https://doi.org/10.1111/j.1523-1755.2004.00517.x

[25]. Inker, L. A.; Schmid, C. H.; Tighiouart, H.; Eckfeldt, J. H.; Feldman, H. I.; Greene, T.; Kusek, J. W.; Manzi, J.; VanLente, F.; Zhang, Y. L.; Coresh, J.; Levey, A. S. N. Engl. J. Med. 2012, 367, 20-29.
https://doi.org/10.1056/NEJMoa1114248

[26]. Dönmez, O.; Korkmaz, H. A.; Yıldız, N.; Ediz, B. Renal failure 2015, 37(5), 784-790.
https://doi.org/10.3109/0886022X.2015.1014771

[27]. Bashier, A. M.; Fadlallah, A. A. S.; Alhashemi, N.; Thadani, P. M.; Abdelgadir, E.; Rashid, F. Adv. Endocrinol. 2015, ID: 254042, 1-8.

[28]. LeBricon, T.; Thervet, E.; Benlakehal, M.; Bousquet, B.; Legendre, C.; Erlich, D. Clin Chem. 1999, 45(12), 2243-2249.

[29]. Tate, J.; Ward, G. Clin. Biochem. Rev. 2004, 25(2), 105-120.

[30]. Sunde, K.; Nilsen, T.; Flodin, M. Scand. J. Clin. Lab. Invest. 2009, 70, 300-304.

[31]. Flodin, M.; Hansson, L. O.; Larsson, A. Clin. Chem. Lab. Med. 2006, 44(12), 1481-5.
https://doi.org/10.1515/CCLM.2006.271

[32]. Furmanova, V.; Vorsek, V.; Cernikova, B.; Palicka, V. Klin. Biochem. Metabol. 2009, 17(4), 233-238.

Supporting Agencies

King Abdul Aziz City for Science and Technology (KACST), Grant# SP-36-16.
Most read articles by the same author(s)
TrendMD

Dimensions - Altmetric - scite_ - PlumX

Downloads and views

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...
License Terms

License Terms

by-nc

Copyright © 2025 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at https://www.eurjchem.com/index.php/eurjchem/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (https://www.eurjchem.com/index.php/eurjchem/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).