European Journal of Chemistry 2017, 8(4), 378-383. doi:10.5155/eurjchem.8.4.378-383.1658

New HPLC method for determination of cystatin C biomarker in human blood


Othman Ibrahem AlMusaimi (1,*) , Abd-Alhakeem Hasan Abu-Nawwas (2) , Danah Mahdi AlShaer (3) , Nabeel Yousef Khaleel (4)

(1) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(2) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(3) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(4) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(*) Corresponding Author

Received: 04 Oct 2017, Accepted: 25 Oct 2017, Published: 31 Dec 2017

Abstract


A rapid and sensitive method for determination of cystatin C (CC) protein in human blood by means of high performance liquid chromatography (HPLC) was developed and validated. Estimated glomerular filtration rate (eGFR) has been calculated for the patients with chronic kidney disease (CKD). Ion pair liquid chromatography technique was utilized to separate CC along with UV detection. Calibration curve with excellent correlation (r2 = 0.99) over the range from 0.75 to 20.50 mg/L of CC was accomplished. Limits of detection and quantify-cation were 0.375 and 0.75 mg/L of CC, respectively. The recoveries were in the range of 93.6-102.3%. Intra-assay and inter-assay variabilities were 8.2 and 6.14%, respectively.


Keywords


Cystatin C; Biomarker; Kidney failure; Chromatography; Glomerular filtration rate; Determination of cystatin C

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DOI: 10.5155/eurjchem.8.4.378-383.1658

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[1]. O. Al Musaimi, A.H. Abu-Nawwas, D. Al Shaer, N.Y. Khaleel, M. Fawzi
Influence of age, gender, smoking, diabetes, thyroid and cardiac dysfunctions on cystatin C biomarker
Medicina de Familia. SEMERGEN  45(1), 44, 2019
DOI: 10.1016/j.semerg.2018.07.005
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[2]. Wenying Zhao, Zhiqiang Wu, Zhixian Fan, Shuguang Xiang
Determination of formic, acetic, propionic, and n-butyric acids by indirect photometric chromatography
Journal of Liquid Chromatography & Related Technologies  41(9), 481, 2018
DOI: 10.1080/10826076.2018.1465434
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How to cite


AlMusaimi, O.; Abu-Nawwas, A.; AlShaer, D.; Khaleel, N. Eur. J. Chem. 2017, 8(4), 378-383. doi:10.5155/eurjchem.8.4.378-383.1658
AlMusaimi, O.; Abu-Nawwas, A.; AlShaer, D.; Khaleel, N. New HPLC method for determination of cystatin C biomarker in human blood. Eur. J. Chem. 2017, 8(4), 378-383. doi:10.5155/eurjchem.8.4.378-383.1658
AlMusaimi, O., Abu-Nawwas, A., AlShaer, D., & Khaleel, N. (2017). New HPLC method for determination of cystatin C biomarker in human blood. European Journal of Chemistry, 8(4), 378-383. doi:10.5155/eurjchem.8.4.378-383.1658
AlMusaimi, Othman, Abd-Alhakeem Hasan Abu-Nawwas, Danah Mahdi AlShaer, & Nabeel Yousef Khaleel. "New HPLC method for determination of cystatin C biomarker in human blood." European Journal of Chemistry [Online], 8.4 (2017): 378-383. Web. 17 Sep. 2019
AlMusaimi, Othman, Abu-Nawwas, Abd-Alhakeem, AlShaer, Danah, AND Khaleel, Nabeel. "New HPLC method for determination of cystatin C biomarker in human blood" European Journal of Chemistry [Online], Volume 8 Number 4 (31 December 2017)

DOI Link: https://doi.org/10.5155/eurjchem.8.4.378-383.1658

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