New HPLC method for determination of cystatin C biomarker in human blood

(1) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(2) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(3) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(4) Department of Chemistry, University of Hail, Hail, 81412, Kingdom of Saudi Arabia
(*) Corresponding Author

Received: 04 Oct 2017, Accepted: 25 Oct 2017, Published: 31 Dec 2017

Abstract

A rapid and sensitive method for determination of cystatin C (CC) protein in human blood by means of high performance liquid chromatography (HPLC) was developed and validated. Estimated glomerular filtration rate (eGFR) has been calculated for the patients with chronic kidney disease (CKD). Ion pair liquid chromatography technique was utilized to separate CC along with UV detection. Calibration curve with excellent correlation (r² = 0.99) over the range from 0.75 to 20.50 mg/L of CC was accomplished. Limits of detection and quantify-cation were 0.375 and 0.75 mg/L of CC, respectively. The recoveries were in the range of 93.6-102.3%. Intra-assay and inter-assay variabilities were 8.2 and 6.14%, respectively.

Keywords

Cystatin C; Biomarker; Kidney failure; Chromatography; Glomerular filtration rate; Determination of cystatin C

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DOI: 10.5155/eurjchem.8.4.378-383.1658

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Citations

[1]. O. Al Musaimi, A.H. Abu-Nawwas, D. Al Shaer, N.Y. Khaleel, M. Fawzi
Influence of age, gender, smoking, diabetes, thyroid and cardiac dysfunctions on cystatin C biomarker
Medicina de Familia. SEMERGEN 45(1), 44, 2019
DOI: 10.1016/j.semerg.2018.07.005

[2]. Wenying Zhao, Zhiqiang Wu, Zhixian Fan, Shuguang Xiang
Determination of formic, acetic, propionic, and n-butyric acids by indirect photometric chromatography
Journal of Liquid Chromatography & Related Technologies 41(9), 481, 2018
DOI: 10.1080/10826076.2018.1465434

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How to cite

AlMusaimi, O.; Abu-Nawwas, A.; AlShaer, D.; Khaleel, N. Eur. J. Chem. 2017, 8(4), 378-383. doi:10.5155/eurjchem.8.4.378-383.1658

AlMusaimi, O.; Abu-Nawwas, A.; AlShaer, D.; Khaleel, N. New HPLC method for determination of cystatin C biomarker in human blood. Eur. J. Chem. 2017, 8(4), 378-383. doi:10.5155/eurjchem.8.4.378-383.1658

AlMusaimi, O., Abu-Nawwas, A., AlShaer, D., & Khaleel, N. (2017). New HPLC method for determination of cystatin C biomarker in human blood. European Journal of Chemistry, 8(4), 378-383. doi:10.5155/eurjchem.8.4.378-383.1658

AlMusaimi, Othman, Abd-Alhakeem Hasan Abu-Nawwas, Danah Mahdi AlShaer, & Nabeel Yousef Khaleel. "New HPLC method for determination of cystatin C biomarker in human blood." European Journal of Chemistry [Online], 8.4 (2017): 378-383. Web. 17 Sep. 2019

AlMusaimi, Othman, Abu-Nawwas, Abd-Alhakeem, AlShaer, Danah, AND Khaleel, Nabeel. "New HPLC method for determination of cystatin C biomarker in human blood" European Journal of Chemistry [Online], Volume 8 Number 4 (31 December 2017)

DOI Link: https://doi.org/10.5155/eurjchem.8.4.378-383.1658

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