OPEN ACCESS | PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS
Determination of antihypertensive drugs by using ratio difference spectrophotometric method
Nessreen Salah Abdelhamid (1) , Eglal Abdelhamid Abd El Aleem El Aleem (2) , Aml Mohamed Khorshed (3) , Mahmoud Mohsen Amin (4,*)
(1) Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt
(2) Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt
(3) Analytical Chemistry Department, Faculty of Pharmacy, Nahda University, Sharq El Nile Beni-Suef, 62511, Egypt
(4) Analytical Chemistry Department, Faculty of Pharmacy, Nahda University, Sharq El Nile Beni-Suef, 62511, Egypt
(*) Corresponding Author
Received: 01 Jul 2018 | Revised: 03 Jan 2019 | Accepted: 07 Jan 2019 | Published: 31 Mar 2019 | Issue Date: March 2019
An accurate, sensitive and time saving spectrophotometric method has been developed and validated for the determination of two antihypertensive drug mixtures. Mixture 1 contains spironolactone (SPIR), furosemide (FUR) and anthranilic acid (ANTH) (impurity of furosemide) and mixture 2 contains triamterene (TRI), hydrochlorothiazide (HCZ) and chlorothiazide (CZ) (impurity of hydrochlorothiazide). In mixture 1, the determination of drugs depends on dividing the spectrum of ternary mixture by the spectrum of 10 µg/mL of standard furosemide and then spironolactone and anthranilic acid were determined using the difference in amplitude between 242.3 and 254.6 nm, and between 250.8 and 242.4 nm in the ratio spectrum, respectively. On the other hand, furosemide could be determined by dividing the spectrum of ternary mixture by the spectrum of 10 µg/mL of standard spironolactone and then it was determined using the difference in amplitude between 244.8 and 229.7 nm in the ratio spectrum. In mixture 2, the determination of drugs depends on dividing the spectrum of ternary mixture by spectrum of 10 µg/mL of standard triamterene and then hydrochlorothiazide and chlorothiazide were determined using the difference in amplitude between 268.9 and 232.8 nm, and between 292.9 and 250.7 nm in the ratio spectrum, respectively. On the other hand, triamterene could be determined by dividing spectrum of ternary mixture by spectrum of 10 µg/mL of standard hydrochlorothiazide and then triamterene was determined using the difference in amplitude between 230.1 and 244 nm in the ratio spectrum. The developed analytical methods were validated regarding good accuracy and precision according to The International Conference on Harmonisation guidelines, and they were applied to pharmaceutical preparations in addition to laboratory prepared mixtures successfully. Statistically the results were compared with those obtained by reported method and no significant difference was found.
Links for Article
| | | | |
| | | | | | |
Article MetricsThis Abstract was viewed 336 times | PDF Article downloaded 121 times
. Ram, V. R.; Dave, P. N.; Joshi, H. S. J. Chromatog. Sci. 2012, 50, 721-726.
. National Center for Biotechnology Information. Pub Chem Compound Database; CID=227, https://pubchem.ncbi.nlm.nih.gov/compound/227 (accessed Oct. 14, 2018).
. Bosch, M. E.; Sanchez, A. J. R.; Rojas, F. S.; Ojeda, C. B. Int. J. Pharm. Bio. Sci. 2013, 3(4), 168-181.
. Hassouna, M. E. M.; Issa, Y. M.; Zayed, A. G. Forensic Res. Criminol. Int. J. 2015, 1(6), 0003, 1-10.
. Patel, H.; Solanki, S. Int. J. Pharm. Pharm. Sci. 2012, 4, 383-386.
. Sanchez, F. G.; Gutierrez, A. F.; Blanco, C. C. Anal. Chim. Acta 1995, 306(2-3), 313-321.
. Maulik, B.; Ketan, D.; Shital, F. J. Pharmaceut. Sci. Biosci. Res. 2012, 2, 144-147.
. Abd-El-Hay, S. S.; Hashem, H.; Gouda, A. A. Acta Pharmaceut. 2016, 66(1), 109-118.
. Naguib, I. A.; Abdelaleem, E. A.; Draz, M. E.; Zaazaa H. E. Spectrochim. Acta A 2014, 130, 350-356.
. Jonczyk, A.; Nowakowska, Z. Acta Poloniae Pharma. 2001, 58(5), 339-344.
. ICH, Q2 (R1) Validation of Analytical Procedures: Text and Methodology, ICH Harmonized Tripartite Guideline, 2005.
How to cite
DOI Link: https://doi.org/10.5155/eurjchem.10.1.12-18.1768
| | | | |
| | | | | |
Save to Zotero Save to Mendeley
European Journal of Chemistry 2019, 10(1), 12-18 | doi: https://doi.org/10.5155/eurjchem.10.1.12-18.1768 | Get rights and content
- There are currently no refbacks.
Copyright (c) 2019 Authors
This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at http://www.eurjchem.com/index.php/eurjchem/pages/view/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (http://www.eurjchem.com/index.php/eurjchem/pages/view/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).
© Copyright 2019 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2019 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 4614 Lavista road, Tucker, GA, 30084, USA. Registered in USA.