European Journal of Chemistry

Composition, antioxidant and anti-inflammatory activities of different polarity extracts of Artemisia nilagirica collected from hilly areas in the Himalayan terrain of Uttarakhand

Crossmark


Main Article Content

Vaishali Garia
Shiv Kumar Dubey
Ananya Bahuguna
Ravendra Kumar
Om Prakash
Dharmendra Singh Rawat

Abstract

The plant Artemesia nilagirica, collected from the terrain of the Himalayan region in Uttarakhand, India, was evaluated for its phytochemical composition, antioxidant and anti-inflammatory activities in vitro. The different polarity extracts of the plant were prepared and subjected to GC-MS analysis for their phytochemical composition. Twenty-six compounds were identified in the hexane extract of Artemisia nilagirica that represents 73.30% of the total area. The main compounds were tetracontane (15.21%), heneicosane (6.52%), and phytyl tetradecanoate (5.11%). The methanol extract yielded 26 compounds, accounting for 83.78% of the total compounds detected. The main compounds were palmitic acid (13.25%), alpha-linolenic acid (10.32%), oleamide (9.41%), phytol (8.58%), muco-inositol (7.27%), and neophytadiene (5.05%). The hexane and methanol extracts showed significant metal chelating activity having IC50 values of 2.23±0.01 and 2.41±0.02 µg/mL, respectively, with standard EDTA having an IC50 value of 2.22±0.01 µg/mL. The methanol extract showed a better anti-inflammatory property having an IC50 value of 2.97±0.03 µg/mL compared to the standard diclofenac potassium having an IC50 value of 3.79±0.01 µg/mL. The hexane extract showed better antioxidant activity in terms of metal chelating activity and reducing power activity than the methanol extract. The anti-inflammatory activity of both hexane and methanol extracts showed better results than the standard marketed drug diclofenac potassium. The current study reveals that Artemisia nilagirica plant extracts have potent antioxidant and anti-inflammatory activities. The effective biological compounds of plant extracts, such as phenols and flavonoids, can be potential alternatives to standard pharmaceuticals.


icon graph This Abstract was viewed 362 times | icon graph Article PDF downloaded 143 times

How to Cite
(1)
Garia, V.; Dubey, S. K.; Bahuguna, A.; Kumar, R.; Prakash, O.; Rawat, D. S. Composition, Antioxidant and Anti-Inflammatory Activities of Different Polarity Extracts of Artemisia Nilagirica Collected from Hilly Areas in the Himalayan Terrain of Uttarakhand. Eur. J. Chem. 2023, 14, 323-329.

Article Details

Share
Crossref - Scopus - Google - European PMC
References

[1]. Seidel, V. Plant-derived chemicals: A source of inspiration for new drugs. Plants 2020, 9, 1562.
https://doi.org/10.3390/plants9111562

[2]. Saini, A. Physicians of ancient India. J. Family Med. Prim. Care 2016, 5, 254-258.
https://doi.org/10.4103/2249-4863.192322

[3]. Phaniendra, A.; Jestadi, D. B.; Periyasamy, L. Free radicals: properties, sources, targets, and their implication in various diseases. Indian J. Clin. Biochem. 2015, 30, 11-26.
https://doi.org/10.1007/s12291-014-0446-0

[4]. Krishnaiah, D.; Sarbatly, R.; Nithyanandam, R. A review of the antioxidant potential of medicinal plant species. Food Bioprod. Process. 2011, 89, 217-233.
https://doi.org/10.1016/j.fbp.2010.04.008

[5]. Goswami, H. K.; Sen, K.; Mukhopadhyay, R. Pteridophytes: evolutionary boon as medicinal plants. Plant Genet. Resour. 2016, 14, 328-355.
https://doi.org/10.1017/S1479262116000290

[6]. Garcia-Oliveira, P.; Barral, M.; Carpena, M.; Gullón, P.; Fraga-Corral, M.; Otero, P.; Prieto, M. A.; Simal-Gandara, J. Traditional plants from Asteraceae family as potential candidates for functional food industry. Food Funct. 2021, 12, 2850-2873.
https://doi.org/10.1039/D0FO03433A

[7]. Rolnik, A.; Olas, B. The plants of the Asteraceae family as agents in the protection of human health. Int. J. Mol. Sci. 2021, 22, 3009.
https://doi.org/10.3390/ijms22063009

[8]. Sharifi-Rad, J.; Sureda, A.; Tenore, G. C.; Daglia, M.; Sharifi-Rad, M.; Valussi, M.; Tundis, R.; Sharifi-Rad, M.; Loizzo, M. R.; Ademiluyi, A. O.; Sharifi-Rad, R.; Ayatollahi, S. A.; Iriti, M. Biological activities of essential oils: From plant chemoecology to traditional healing systems. Molecules 2017, 22, 70.
https://doi.org/10.3390/molecules22010070

[9]. Cala, A. C.; Chagas, A. C. S.; Oliveira, M. C. S.; Matos, A. P.; Borges, L. M. F.; Sousa, L. A. D.; Souza, F. A.; Oliveira, G. P. In vitro anthelmintic effect of Melia azedarach L. and Trichilia claussenii C. against sheep gastrointestinal nematodes. Exp. Parasitol. 2012, 130, 98-102.
https://doi.org/10.1016/j.exppara.2011.12.011

[10]. Bahuguna, A.; Dubey, S. K.; Garia, V.; Kumar, R.; Prakash, O.; Rawat, D. S. Composition, antioxidant and anti-inflammatory activities of different polarity extracts of Anaphalis busua from the Himalayan terrain of Uttarakhand. Eur. J. Chem. 2023, 14, 114-120.
https://doi.org/10.5155/eurjchem.14.1.114-120.2395

[11]. McLafferty, F. W. Wiley registry of mass spectral data, 9th ed. With nist 2011; John Wiley & Sons, 2011.

[12]. Adams, R. P. Identification of essential oil components by gas chromatography/mass spectrometry; Allured Publishing Corporation: Carol Stream, 2007.

[13]. Huang, H.-C.; Wang, H.-F.; Yih, K.-H.; Chang, L.-Z.; Chang, T.-M. The dual antimelanogenic and antioxidant activities of the essential oil extracted from the leaves of Acorus macrospadiceus (Yamamoto) F. n. wei et Y. k. li. Evid. Based. Complement. Alternat. Med. 2012, 2012, 781280.
https://doi.org/10.1155/2012/781280

[14]. Yen, G. Antioxidant activity of anthraquinones and anthrone. Food Chem. 2000, 70, 437-441.
https://doi.org/10.1016/S0308-8146(00)00108-4

[15]. Pavithra, K.; Vadivukkarasi, S. Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima (Jacq.) Cogn. Food Sci. Hum. Wellness 2015, 4, 42-46.
https://doi.org/10.1016/j.fshw.2015.02.001

[16]. Kar, B.; Kumar, R. B. S.; Karmakar, I.; Dola, N.; Bala, A.; Mazumder, U. K.; Hadar, P. K. Antioxidant and in vitro anti-inflammatory activities of Mimusops elengi leaves. Asian Pac. J. Trop. Biomed. 2012, 2, S976-S980.
https://doi.org/10.1016/S2221-1691(12)60346-3

[17]. Bewick, V.; Cheek, L.; Ball, J. Statistics review 9: one-way analysis of variance. Crit. Care 2004, 8, 130-136.
https://doi.org/10.1186/cc2836

[18]. Amarowicz, R.; Pegg, R. B.; Rahimi-Moghaddam, P.; Barl, B.; Weil, J. A. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem. 2004, 84, 551-562.
https://doi.org/10.1016/S0308-8146(03)00278-4

[19]. Gairola, K.; Gururani, S.; Kumar, R.; Prakash, O.; Agrawal, S.; Dubey, S. K. Composition, Antioxidant and Anti-inflammatory activities of Hexane and Methanol extracts of Acmella uliginosa from Terai region of Uttarakhand. Braz. J. Pharm. Sci. 2022, 58.
https://doi.org/10.1590/s2175-97902022e20353

[20]. Al-Rajhi, A. M. H.; Qanash, H.; Almuhayawi, M. S.; Al Jaouni, S. K.; Bakri, M. M.; Ganash, M.; Salama, H. M.; Selim, S.; Abdelghany, T. M. Molecular interaction studies and phytochemical characterization of Mentha pulegium L. constituents with multiple biological utilities as antioxidant, antimicrobial, anticancer and anti-hemolytic agents. Molecules 2022, 27.
https://doi.org/10.3390/molecules27154824

[21]. Desta, M.; Molla, A.; Yusuf, Z. Characterization of physico-chemical properties and antioxidant activity of oil from seed, leaf and stem of purslane (Portulaca oleracea L.). Biotechnol. Rep. (Amst.) 2020, 27, e00512.
https://doi.org/10.1016/j.btre.2020.e00512

[22]. Arora, S.; Meena, S. Pharmagnostic potentialities of ceropegia bulbosa roxb. Var. Lushii (grah.) Hook.f.: an endangered plant from thar desert, Rajasthan, India. Int. Res. J. Pharm. 2017, 8, 77-82.
https://doi.org/10.7897/2230-8407.086100

[23]. Kim, S.-K.; Karadeniz, F. Biological importance and applications of squalene and squalane. Adv. Food Nutr. Res. 2012, 65, 223-233.
https://doi.org/10.1016/B978-0-12-416003-3.00014-7

[24]. Swamy, M. K.; Arumugam, G.; Kaur, R.; Ghasemzadeh, A.; Yusoff, M. M.; Sinniah, U. R. GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves. Evid. Based. Complement. Alternat. Med. 2017, 2017, 1517683.
https://doi.org/10.1155/2017/1517683

[25]. Ibrahim, M.; Oyebanji, E.; Fowora, M.; Aiyeolemi, A.; Orabuchi, C.; Akinnawo, B.; Adekunle, A. A. Extracts of endophytic fungi from leaves of selected Nigerian ethnomedicinal plants exhibited antioxidant activity. BMC Complement. Med. Ther. 2021, 21, 98.
https://doi.org/10.1186/s12906-021-03269-3

[26]. Silva, R. O.; Sousa, F. B. M.; Damasceno, S. R. B.; Carvalho, N. S.; Silva, V. G.; Oliveira, F. R. M. A.; Sousa, D. P.; Aragão, K. S.; Barbosa, A. L. R.; Freitas, R. M.; Medeiros, J. V. R. Phytol, a diterpene alcohol, inhibits the inflammatory response by reducing cytokine production and oxidative stress. Fundam. Clin. Pharmacol. 2014, 28, 455-464.
https://doi.org/10.1111/fcp.12049

[27]. Akbari, S.; Abdurahman, N. H.; Yunus, R. M.; Alara, O. R.; Abayomi, O. O. Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil. Mater. Sci. Energy Technol. 2019, 2, 349-355.
https://doi.org/10.1016/j.mset.2018.12.001

Supporting Agencies

Advanced Instrumentation Research Facility of Jawaharlal Nehru University (JNU), New Delhi, India and Department of Biochemistry, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India.
Most read articles by the same author(s)

Most read articles by the same author(s)

TrendMD

Dimensions - Altmetric - scite_ - PlumX

Downloads and views

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...
License Terms

License Terms

by-nc

Copyright © 2024 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at https://www.eurjchem.com/index.php/eurjchem/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (https://www.eurjchem.com/index.php/eurjchem/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).