European Journal of Chemistry 2011, 2(2), 276-281 | doi: https://doi.org/10.5155/eurjchem.2.2.276-281.401 | Get rights and content






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Fundamentals of micellar electrokinetic chromatography (MEKC)


Syed Asad Ali Rizvi (1,*) , Duc Phuc Do (2) , Ayman Mahmoud Saleh (3)

(1) School of Pharmacy, Lake Erie College of Osteopathic Medicine (LECOM), Erie, PA 16509, USA
(2) Department of Pharmaceutical Sciences, College of Pharmacy, Chicago State University, Chicago, IL 60628, USA
(3) Department of Biochemistry and Molecular Medicine, School of Medicine, Lake Erie College of Osteopathic Medicine (LECOM), Erie, PA 16509, USA
(*) Corresponding Author

Received: 04 Feb 2011 | Accepted: 24 Feb 2011 | Published: 30 Jun 2011 | Issue Date: June 2011

Abstract


Micellar electrokinetic chromatography (MEKC) is a useful branch of capillary electrophoresis (CE) that utilizes surfactant above critical micelle concentration (CMC) as pseudo-stationary phase. MEKC can be employed to separate both charged and neutral molecules, individually or simultaneously, including chiral compounds. MECK benefits from high peak efficiency due to electroosmotic flow (EOF) in the separation capillary, compounded with large variety of synthetic surfactants, organic modifiers, temperature and variable separation voltage has made MECK the method of choice for separation scientists. In this review, we present the introduction of CE, fundamentals of surfactant chemistry as it relates to MEKC, separation principles in MECK including equations involved in calculating separation parameters (capacity factor, resolution etc.).

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Keywords


Capillary electrophoresis; Electroosmotic flow; Micellar electrokinetic chromatography; Surfactants; Critical micelle concentration; Polymeric surfactants

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DOI: 10.5155/eurjchem.2.2.276-281.401

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References

[1]. http://old.iupac.org/goldbook/C01075.pdf

[2]. Tiselius, A. The Moving Boundary Method of Studying the Electrophoresis of Proteins, Ph.D. Thesis, Nova Acta Regiae Societatis Scientiarum Upsaliensis, Ser. IV, Vol. 17, No.4, Almqvist and Wiksell, Uppsala, Sweden, 1930.

[3]. Jorgenson, J. W.; Lukacs, K. D. Clin. Chem. 1981, 27, 1551-1553.
PMid:7261333

[4]. Jorgenson, J. W.; Lukacs, K. D. J. High Resolut. Chromatogr. 1981, 4, 230-231.
doi:10.1002/jhrc.1240040507

[5]. Terabe, S.; Otsuka, K.; Ando, T. Anal. Chem. 1989, 61, 251-260.
doi:10.1021/ac00178a013

[6]. Terabe, S.; Otsuka, K.; Ando, T. Anal. Chem. 1985, 57, 834-841.
doi:10.1021/ac00281a014

[7]. Barret, D. G.; Gellman, S. H. J. Am. Chem. Soc. 1993, 115, 9343-9344.
doi:10.1021/ja00073a082

[8]. Fuhrhop, J. H.; Gellman, S. H. Acc. Chem. Res. 1986, 19, 130-137.
doi:10.1021/ar00125a002

[9]. Clint, J. H. Surfactant Aggregation, Blackie & Sons, Glasgow, 1992.

[10]. Gill, S. J.; Wadso, I. Proc. Natl. Acad. Sci. USA. 1976, 73, 2955-2958.
doi:10.1073/pnas.73.9.2955

[11]. Tanford, C. The hydrophobic effect: formation of micelles and biological membranes, John Wiley & Sons, New York, 1980.

[12]. Lange, K. R. Surfactants: A Practical Handbook, Hanser Gardner Publications, Ohio, 1999.

[13]. Rosen, M. J. Surfactants and Interfacial Phenomena, Wiley-Interscience, NY, 2004.
doi:10.1002/0471670561

[14]. Notter, R. H. Lung Surfactants: Basic Science and Clinical Applications, CRC, Boca Raton, FL, 2000.

[15]. Kallay, N. Interfacial Dynamics, CRC, Boca Raton, FL, 2000.

[16]. Tsujii, K. Surface Activity: Pronciles, Phenomena, and Applications (Polymers, Interfaces and Biomaterials), Academic Press. San Diego, CA, 1998.

[17]. Myers, D. Surfactant Science and Technology, John Wiley & Sons, NJ, 2005.
doi:10.1002/047174607X

[18]. Holmberg, K.; Jönsson, B.; Kronberg, B.; Lindman, B. Surfactants and Polymers in Aqueous Solution, John Wiley & Sons, NY, 2002.
doi:10.1002/0470856424

[19]. Jones, M. N.; Chapman, D. Micelles, Monolayers, and Biomembranes, Wiley-Liss, NY, 1994.

[20]. Stache, H. W. Anionic Surfactants, CRC, Boca Raton, FL, 1995.

[21]. Van Os, N. M.; Haak, J. R.; Rupert, L. A. M. Physico-Chemical Properties of Selected Anionic, Cationic and Nonionic Surfactants, Elsevier Science Pub Co, NY, 1993.

[22]. Rubingh, D. Cationic Surfactants, CRC, Boca Raton, FL, 1990.

[23]. Cross, J. Cationic Surfactants, CRC, Boca Raton, FL, 1994.

[24]. Schramm, L. L. Surfactants: Fundamentals and Applications in the Petroleum Industry, Cambridge University Press, Cambridge, UK, 2000.
doi:10.1017/CBO9780511524844

[25]. Holmberg, K. Novel Surfactants: Preparation, Applications, and Biodegradability, CRC, Boca Raton, FL, 2003.
doi:10.1201/9780203911730

[26]. Lomax, E. G. Amphoteric Surfactants, CRC, Boca Raton, FL, 1996.

[27]. Rieger, M. Surfactants in Cosmetics, CRC, Boca Raton, FL, 1997.

[28]. Schick. M. J. Nonionic Surfactants, CRC, Boca Raton, FL, 1987.

[29]. Van Os, N. M. Nonionic Surfactants, CRC, Boca Raton, FL, 1997.

[30]. Patist, A.; Kanicky, J. R.; Shukla, P.; Shah, D. O. J. Colloid Interface Sci. 2002, 245, 1-15.
doi:10.1006/jcis.2001.7955
PMid:16290329

[31]. Hiemenz, P. C.; Rajagopalan, R. Principles of Colloid and Surface Chemistry, Dekker, New York, 1997.

[32]. Hunter, R, J. Foundations of Colloid Science, Oxford Univ. Press, New York, 1987.

[33]. Tanford, C. The Hydrophobic Effect. The Formation of Micelles and Biological Membranes, Wiley, New York, 1980.

[34]. McBain, J. W. Trans. Faraday Soc. 1913, 9, 99-101.

[35]. McBain, J. W.; Salmon, C. S. J. Am. Chem. Soc. 1920, 42, 426-436.
doi:10.1021/ja01448a010

[36]. Hartley, G. S. Aqueous Solutions of Paraffin-Chain Salts, Hermann, Paris, 1936.

[37]. Hartley, G. S.; Collie, B.; Samis, C. S. Trans. Faraday Soc. 1936, 32, 795-815.
doi:10.1039/tf9363200795

[38]. McBain, J. W.; Martin, H. E. J. Chem. Soc. 1914, 105, 957-977.

[39]. Debye, P.; Anacker, E. W. J. Phys. Colloid. Chem. 1951, 55, 644-655.

[40]. Menger, F. M. Angew. Chem. Int. Ed. Engl. 1991, 30, 1086-1099.
doi:10.1002/anie.199110861

[41]. Terabe, S. Anal. Chem. 2004, 76, 240A-246A.
doi:10.1021/ac0415859

[42]. Sandra, P.; Vindevogel, J. Introduction to Micellar Electrokinetic Chromatography, Huthig Pub Ltd, Verlag, Heidelberg, 1992, pp 43-67.

[43]. Khaledi, M. G. (Ed.), High-Performance Capillary Electrophoresis: Theory, Techniques, and Applications, Wiley-Interscience, New York, 1998, pp 77-131.

[44]. Lander, J. P. (Ed.), Handbook of Capillary Electrophoresis, Second Edition, CRC-Press, Florida, 1996, pp 49-74.

[45]. Piirma, I. Polymeric Surfactants, Marcel Dekker, Inc, New York, 1992.

[46]. Gambogi, R. J.; Blum, F. D. J. Colloid Interface Sci. 1990, 140, 525-534.
doi:10.1016/0021-9797(90)90372-U

[47]. Palmer, C. P.; Terabe, S. Anal. Chem. 1997, 69, 1852-1860.
doi:10.1021/ac960801d

[48]. Palmer, C. P. J. Chromatogr. A. 1997, 780, 75-92.
doi:10.1016/S0021-9673(97)00367-1

[49]. Shamsi, S. A.; Warner, I. M. Electrophoresis 1997, 18, 853-872.
doi:10.1002/elps.1150180604
PMid:9221871

[50]. Ward, T. J.; Hamburg, D. M. Anal. Chem. 2004, 76, 4635-4644.
doi:10.1021/ac040093t
PMid:15307771

[51]. Palmer, C. P.; McCarney, J. P. Electrophoresis 2004, 25, 4086-4094.
doi:10.1002/elps.200406110
PMid:15597413

[52]. Palmer, C. P.; McCarney, J. P. J. Chromatogr. A 2004, 1044, 159-176.
doi:10.1016/j.chroma.2004.04.068
PMid:15354436

[53]. Rundlett, K. L.; Armstrong, D. W. Anal. Chem. 1996, 68, 3493-3497.
doi:10.1021/ac960472p
PMid:21619282

[54]. Liu, H.; Farrell, S.; Uhrich, U. J. Control. Release. 2000, 68, 167-174.
doi:10.1016/S0168-3659(00)00247-9

[55]. Camilleri, P. Capillary Electrophoresis: THEORY and PRACTICE, CRC Press, Florida, USA, 1998, pp 217-218.

[56]. Chankvetadze, B. Capillary Electrophoresis In Chiral Analysis, John Wiley & Sons, Ltd, West Sussex, England, 1997.

How to cite


Rizvi, S.; Do, D.; Saleh, A. Eur. J. Chem. 2011, 2(2), 276-281. doi:10.5155/eurjchem.2.2.276-281.401
Rizvi, S.; Do, D.; Saleh, A. Fundamentals of micellar electrokinetic chromatography (MEKC). Eur. J. Chem. 2011, 2(2), 276-281. doi:10.5155/eurjchem.2.2.276-281.401
Rizvi, S., Do, D., & Saleh, A. (2011). Fundamentals of micellar electrokinetic chromatography (MEKC). European Journal of Chemistry, 2(2), 276-281. doi:10.5155/eurjchem.2.2.276-281.401
Rizvi, Syed, Duc Phuc Do, & Ayman Mahmoud Saleh. "Fundamentals of micellar electrokinetic chromatography (MEKC)." European Journal of Chemistry [Online], 2.2 (2011): 276-281. Web. 20 Jan. 2020
Rizvi, Syed, Do, Duc, AND Saleh, Ayman. "Fundamentals of micellar electrokinetic chromatography (MEKC)" European Journal of Chemistry [Online], Volume 2 Number 2 (30 June 2011)

DOI Link: https://doi.org/10.5155/eurjchem.2.2.276-281.401

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