Fundamentals of micellar electrokinetic chromatography (MEKC)

Syed Asad Ali Rizvi; Duc Phuc Do; Ayman Mahmoud Saleh

doi:10.5155/eurjchem.2.2.276-281.401

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Published: Jun 30, 2011

DOI: https://doi.org/10.5155/eurjchem.2.2.276-281.401

Keywords:

Capillary electrophoresis, Electroosmotic flow, Micellar electrokinetic chromatography, Surfactants, Critical micelle concentration, Polymeric surfactants

Section

Review Article

Issue

Vol. 2 No. 2 (2011): June 2011

Dates

Received 2011-02-04
Accepted 2011-02-24
Published 2011-06-30

Syed Asad Ali Rizvi

School of Pharmacy, Lake Erie College of Osteopathic Medicine (LECOM), Erie, PA 16509, USA

Duc Phuc Do

Department of Pharmaceutical Sciences, College of Pharmacy, Chicago State University, Chicago, IL 60628, USA

Ayman Mahmoud Saleh

Department of Biochemistry and Molecular Medicine, School of Medicine, Lake Erie College of Osteopathic Medicine (LECOM), Erie, PA 16509, USA

Abstract

Micellar electrokinetic chromatography (MEKC) is a useful branch of capillary electrophoresis (CE) that utilizes surfactant above critical micelle concentration (CMC) as pseudo-stationary phase. MEKC can be employed to separate both charged and neutral molecules, individually or simultaneously, including chiral compounds. MECK benefits from high peak efficiency due to electroosmotic flow (EOF) in the separation capillary, compounded with large variety of synthetic surfactants, organic modifiers, temperature and variable separation voltage has made MECK the method of choice for separation scientists. In this review, we present the introduction of CE, fundamentals of surfactant chemistry as it relates to MEKC, separation principles in MECK including equations involved in calculating separation parameters (capacity factor, resolution etc.).

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Rizvi, S. A. A.; Do, D. P.; Saleh, A. M. Fundamentals of Micellar Electrokinetic Chromatography (MEKC). Eur. J. Chem. 2011, 2, 276-281.

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