European Journal of Chemistry 2011, 2(2), 276-281. doi:10.5155/eurjchem.2.2.276-281.401

Fundamentals of micellar electrokinetic chromatography (MEKC)


Syed Asad Ali Rizvi (1,*) , Duc Phuc Do (2) , Ayman Mahmoud Saleh (3)

(1) School of Pharmacy, Lake Erie College of Osteopathic Medicine (LECOM), Erie, PA 16509, USA
(2) Department of Pharmaceutical Sciences, College of Pharmacy, Chicago State University, Chicago, IL 60628, USA
(3) Department of Biochemistry and Molecular Medicine, School of Medicine, Lake Erie College of Osteopathic Medicine (LECOM), Erie, PA 16509, USA
(*) Corresponding Author

Received: 04 Feb 2011, Accepted: 24 Feb 2011, Published: 30 Jun 2011

Abstract


Micellar electrokinetic chromatography (MEKC) is a useful branch of capillary electrophoresis (CE) that utilizes surfactant above critical micelle concentration (CMC) as pseudo-stationary phase. MEKC can be employed to separate both charged and neutral molecules, individually or simultaneously, including chiral compounds. MECK benefits from high peak efficiency due to electroosmotic flow (EOF) in the separation capillary, compounded with large variety of synthetic surfactants, organic modifiers, temperature and variable separation voltage has made MECK the method of choice for separation scientists. In this review, we present the introduction of CE, fundamentals of surfactant chemistry as it relates to MEKC, separation principles in MECK including equations involved in calculating separation parameters (capacity factor, resolution etc.).

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Keywords


Capillary electrophoresis; Electroosmotic flow; Micellar electrokinetic chromatography; Surfactants; Critical micelle concentration; Polymeric surfactants

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DOI: 10.5155/eurjchem.2.2.276-281.401

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How to cite


Rizvi, S.; Do, D.; Saleh, A. Eur. J. Chem. 2011, 2(2), 276-281. doi:10.5155/eurjchem.2.2.276-281.401
Rizvi, S.; Do, D.; Saleh, A. Fundamentals of micellar electrokinetic chromatography (MEKC). Eur. J. Chem. 2011, 2(2), 276-281. doi:10.5155/eurjchem.2.2.276-281.401
Rizvi, S., Do, D., & Saleh, A. (2011). Fundamentals of micellar electrokinetic chromatography (MEKC). European Journal of Chemistry, 2(2), 276-281. doi:10.5155/eurjchem.2.2.276-281.401
Rizvi, Syed, Duc Phuc Do, & Ayman Mahmoud Saleh. "Fundamentals of micellar electrokinetic chromatography (MEKC)." European Journal of Chemistry [Online], 2.2 (2011): 276-281. Web. 16 Sep. 2019
Rizvi, Syed, Do, Duc, AND Saleh, Ayman. "Fundamentals of micellar electrokinetic chromatography (MEKC)" European Journal of Chemistry [Online], Volume 2 Number 2 (30 June 2011)

DOI Link: https://doi.org/10.5155/eurjchem.2.2.276-281.401

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