European Journal of Chemistry 2012, 3(2), 147-151. doi:10.5155/eurjchem.3.2.147-151.569

Cytotoxic and antioxidant effects of grape seed oil on the treatment of leukemia with methotrexate


Nefise Ozlen Sahin (1,*) , Mehmet Berkoz (2) , Ebru Derici Eker (3) , Bartosz Pomierny (4) , Katarzyna Przejczowska (5)

(1) Mersin University, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Mersin, TR-33169, Turkey
(2) Mersin University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Mersin, TR-33169, Turkey
(3) Mersin University, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Mersin, TR-33169, Turkey
(4) Jagellonian University, Medical College, Krakow, 30-688, Poland
(5) Jagellonian University, Medical College, Krakow, 30-688, Poland
(*) Corresponding Author

Received: 07 Dec 2011, Accepted: 18 Dec 2011, Published: 30 Jun 2012

Abstract


Methotrexate (MTX), a widely used cytotoxic chemotherapeutic agent, is often limited by its severe toxicity. Regarding the mechanisms of its adverse effects, several hypotheses have been put forward, among which oxidative stress is highly noticeable. Additive effect of oxidative damage caused by MTX to oxidative stress induced by cancer makes the situation dramatically bad. In order to reduce the damage, several approaches have been suggested. Grape seed is one of the most significant prophylactic agents due to its antioxidant and bioflavonoids composition. The aim of this study was to investigate the protective effect of grape seed oil against MTX-induced oxidative stress in K-562 human chronic myeloid leukemia cell lines. Cells were divided into groups as following control, GSOH (tumor cells treated with 200 mg/mL of grape seed oil), GSOL (tumor cells treated with 100 mg/mL of grape seed oil), MTX (tumor cells treated with 50 nM methotrexate) and MTX + GSOH ( tumor cells treated with 200 mg/mL of grape seed oil and methotrexate). For antioxidant statue; superoxide dismutase (SOD), catalase (CAT), paraoxonase (PON) and aryl esterase (ARE) activities, for lipid peroxidation; malondialdehyde (MDA) level and also for cytotoxicity; cell viability were detected in 24th and 48th hours of the cell culture incubation. Based on the data, 200 mg/mL of grape seed oil indicates synergic effects with MTX on K562 regarding cytotoxicity especially in 48th hour. In case of GSOH + MTX combined treatment for 24 hours, antioxidant system take part preventing lipid peroxidation and a possible oxidative damage. Upon 48 hour-GSOH treatment, antioxidant parameters show significant increase and hence, prevent lipid peroxidation in cancer cells. In conclusion, GSOH complimentary treatment may be suggested for leukemia therapy with MTX to reduce side effects and enhance the cytotoxicity of MTX.

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Keywords


Leukemia; Cytotoxicity; Methotrexate; K562 cell line; Grape seed oil; Oxidative stress

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DOI: 10.5155/eurjchem.3.2.147-151.569

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[1]. Mayumi Minamisawa, Shoichiro Yoshida, Atsushi Uzawa
The functional evaluation of waste yuzu (Citrus junos) seeds
Food Funct.  5(2), 330, 2014
DOI: 10.1039/C3FO60440C
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[2]. M. de Sá, V. Justino, M. I. Spranger, Y. Q. Zhao, L. Han, B. S. Sun
Extraction Yields and Anti-oxidant Activity of Proanthocyanidins from Different Parts of Grape Pomace: Effect of Mechanical Treatments
Phytochemical Analysis  25(2), 134, 2014
DOI: 10.1002/pca.2479
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How to cite


Sahin, N.; Berkoz, M.; Eker, E.; Pomierny, B.; Przejczowska, K. Eur. J. Chem. 2012, 3(2), 147-151. doi:10.5155/eurjchem.3.2.147-151.569
Sahin, N.; Berkoz, M.; Eker, E.; Pomierny, B.; Przejczowska, K. Cytotoxic and antioxidant effects of grape seed oil on the treatment of leukemia with methotrexate. Eur. J. Chem. 2012, 3(2), 147-151. doi:10.5155/eurjchem.3.2.147-151.569
Sahin, N., Berkoz, M., Eker, E., Pomierny, B., & Przejczowska, K. (2012). Cytotoxic and antioxidant effects of grape seed oil on the treatment of leukemia with methotrexate. European Journal of Chemistry, 3(2), 147-151. doi:10.5155/eurjchem.3.2.147-151.569
Sahin, Nefise, Mehmet Berkoz, Ebru Derici Eker, Bartosz Pomierny, & Katarzyna Przejczowska. "Cytotoxic and antioxidant effects of grape seed oil on the treatment of leukemia with methotrexate." European Journal of Chemistry [Online], 3.2 (2012): 147-151. Web. 17 Sep. 2019
Sahin, Nefise, Berkoz, Mehmet, Eker, Ebru, Pomierny, Bartosz, AND Przejczowska, Katarzyna. "Cytotoxic and antioxidant effects of grape seed oil on the treatment of leukemia with methotrexate" European Journal of Chemistry [Online], Volume 3 Number 2 (30 June 2012)

DOI Link: https://doi.org/10.5155/eurjchem.3.2.147-151.569

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