European Journal of Chemistry

Composition and content of total phenolics and flavonoids, and antioxidant activity of Trigonella isthmocarpa

Article Sidebar

PDF
Published: Mar 31, 2013
DOI: https://doi.org/10.5155/eurjchem.4.1.7-9.719
Keywords:
HPLC, Fabaceae, Total phenol, T. isthmocarpa, Total flavonoid, DPPH radical scavenging activity
Section
Research Article
Issue
Vol. 4 No. 1 (2013): March 2013
Dates
Received 2012-12-21
Accepted 2013-01-07
Published 2013-03-31


Main Article Content

Serife Selma Uras Gungor
Department of Pharmacognosy, Faculty of Pharmacy, Mersin University, Yenişehir Campus, 33169 Mersin, Turkey
Sevda Guzel Ozay
Department of Pharmacognosy, Faculty of Pharmacy, Mersin University, Yenişehir Campus, 33169 Mersin, Turkey
Ahmet Ilcim
Department of Biology, Faculty of Arts and Science, Mustafa Kemal University, 31000, Antakya, Hatay, Turkey
Gamze Kokdil
Department of Pharmacognosy, Faculty of Pharmacy, Mersin University, Yenişehir Campus, 33169 Mersin, Turkey

Abstract

The aqueous methanolic extracts obtained from aerial parts and seeds of T. isthmocarpa Boiss. & Bal. from Turkey were analyzed by reversed-phase HPLC for their phenolic acid and flavonoid composition. Total phenolic content was determined using the Folin-Ciocalteu assay, while total flavonoid content was measured spectrometrically with AlCl3 assay. Primary antioxidant activity in terms of free radical scavenging activities of both extracts was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The highest total flavonoid and total phenolic content was determined in the extract of aerial parts (830.8 mg/100 g and 877.9 mg gallic acid equivalent/100 g, respectively). The extracts of aerial parts and seeds had the high antioxidant activity (87.2 % and 78.2%, respectively). Gallic acid, caffeic acid, luteolin, apigenin and kaempferol were detected as phenolic compounds in both extracts by HPLC methods. In addition, an isoflavone, formononetin was determined in the extract from aerial parts of the plant. The present results suggest that T. isthmocarpa have shown potential a source of natural antioxidants.

4_1_7_9

icon graph This Abstract was viewed 1841 times | icon graph Article PDF downloaded 933 times

How to Cite
(1)
Gungor, S. S. U.; Ozay, S. G.; Ilcim, A.; Kokdil, G. Composition and Content of Total Phenolics and Flavonoids, and Antioxidant Activity of Trigonella Isthmocarpa. Eur. J. Chem. 2013, 4, 7-9.

Article Details

Share
Links for Article


Crossref - Scopus - Google - European PMC
Crossref
Scopus
Google Scholar
Europe PMC
References

[1]. Bown, D. Encyclopedia of Herbs & Their Uses, 1st edition, Darling Kindersley Limited, 2002.

[2]. Evans, W. C. Trease and Evans Pharmacognosy, 15th edition, University of Nottingham, 2002.

[3]. Townsend, C. C. Leguminosae. In Flora of Iraq, The University Press, 1974.
PMCid:1355892

[4]. Basch, E.; Ulbricht, C.; Kuo, G.; Szapary, P.; Smith, M. Altern. Med. Rev. 2003, 8, 20-26.
PMid:12611558

[5]. Srinivasan, K. Food Rev. Int. 2006, 22, 203-224.
http://dx.doi.org/10.1080/87559120600586315

[6]. Pandian, R. S.; Anuradha, C. V.; Viswanathan, P. J. Ethnopharmacol. 2002, 81, 393¬-397.

[7]. Toppo, F. A.; Akhand, R.; Pathak A. K. Asian J. Pharm. Clin. Res. 2009, 2(4), 28-38.

[8]. Yaday, R.; Kaushik, R.; Gupta, D. Inter. J. Eng. Res. Appl. 2011, 1, 32-35.

[9]. Bown Yuldashev, M. P. Chem. Nat. Compd. 2002, 38, 291-292.
http://dx.doi.org/10.1023/A:1020448531522

[10]. Kawashyt, S. A.; Abdalla, M. F.; El Din, E. M. G.; Saleh, N. A. M. Biochem. Syst. Ecol. 1998, 26, 851-856.
http://dx.doi.org/10.1016/S0305-1978(96)00101-9

[11]. Huber-Morath, A. Trigonella L. in: Davis P. H. (ed.), Flora of Turkey and The East Aegean Island, Edinburgh University Press, 1969.

[12]. Davis, P. H.; Mill, R. R.; Tan, K. Flora of Turkey and The East Aegean Island, Edinburgh University Press, 1988.

[13]. Guner, A.; Ozhatay, N.; Ekim, T.; Baser, K. H. C. Leguminosae. Flora of Turkey and The East Aegean Islands (Supplement 2), Edinburgh University Press, 2000.

[14]. Gikas, E.; Bazoti, F. N.; Papadopoulos, N.; Alesta, A.; Economou, G.; Tsarbopoulos, A. Anal. Lett. 2011, 44, 1463-1472.
http://dx.doi.org/10.1080/00032719.2010.512683

[15]. Kim, D. O.; Jeong, S. W.; Lee, C. Y. Food Chem. 2003, 81, 321-326.
http://dx.doi.org/10.1016/S0308-8146(02)00423-5

[16]. Sunhasree, B.; Baskar, R.; Keerthana, R. L.; Susan, R. L.; Rajasekaran, P. Food Chem. 2009, 115, 1213-1220.
http://dx.doi.org/10.1016/j.foodchem.2009.01.029

[17]. Wang, S.; Di, D.; Liu, X.; Jiang, S. J. Liq. Chromatogr. Rel. Technol. 2007, 30, 1991-1999.
http://dx.doi.org/10.1080/10826070701386637

[18]. Khoschsorur, G. A.; Erwa, W. J. Chromatogr. B 2004, 799, 355-360.
http://dx.doi.org/10.1016/j.jchromb.2003.10.074

[19]. Yen, G. C.; Duht, P. D. J. Agr. Food Chem. 1994, 42, 629-632.
http://dx.doi.org/10.1021/jf00039a005

[20]. Mariod, A. A.; Matthaus, B.; Hussein I. H. Int. J. Food Sci. Tech. 2008, 43, 921-926.
http://dx.doi.org/10.1111/j.1365-2621.2007.01543.x

[21]. Zheng, C. D.; Li, G.; Li, H. Q.; Xu, X. J.; Gao, J. M.; Zhang, A. L. Nat. Prod. Commun. 2010, 5, 1759-1765.
PMid:21213975

Supporting Agencies

Advanced Technology of Education, Research and Application Center, Mersin University
Most read articles by the same author(s)
TrendMD

Dimensions - Altmetric - scite_ - PlumX

Downloads and views

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...
License Terms

License Terms

by-nc

Copyright © 2025 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at https://www.eurjchem.com/index.php/eurjchem/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (https://www.eurjchem.com/index.php/eurjchem/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).