European Journal of Chemistry

Electrochemically activated carbon fiber sensors as fast and sensitive adenine metabolite amperometric detectors



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Kholoud Mohammed Abou El-Nour

Abstract

A direct and fast voltammetric method of determination of 2,8-dihydroxyadenine (2,8-DHA), with a novel nanostructured carbon fiber sensor (CFS), is revealed in this work. To achieve high sensitivity required in biological determinations, before the determination of 2,8-DHA, carbon fiber sensors were activated by electrochemical pretreatment method. The electrochemical activation process generates a nanostructured carbon fiber surface, which is stable. The high stability of the active surface, and the high reproducibility of the activation process, leads to reproducible and sensitive fast scan voltammetric (FSV) determinations of 2,8-DHA. The 2,8-DHA sensitivity was determined in 31 mM phosphate buffer and Hank’s Balanced Salt solution (HBSS) : phosphate buffer (1:5) pH = 7.4, with different scan rates of 250, 500 and 1000 V/s. The potential window in the determinations was from -1.0 to 1.5 V (vs. SCE). A high sensitivity of the new method of 2,8-DHA determinations by FSV at 500 V/s was found to be 0.40±0.01 nA µM-1 (r2 = 0.998) from the calibration plot of 2,8-DHA, with a linear dynamic range from 1-20 µM, and a limit of detection of 1 µM under physiological conditions.

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El-Nour, K. M. A. Electrochemically Activated Carbon Fiber Sensors As Fast and Sensitive Adenine Metabolite Amperometric Detectors. Eur. J. Chem. 2013, 4, 162-167.

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References

[1]. Wessel, T.; Lanvers, C.; Fruend, S.; Hempel, G. J. Chromatogr. A 2000, 894, 157-164.
http://dx.doi.org/10.1016/S0021-9673(00)00439-8

[2]. McCaskey, P. C.; Rigsby, W. E.; Hinton, D. M.; Friedlander, L.; Hurst, V. J. Vet. Pathol. 1991, 28, 99-109.
http://dx.doi.org/10.1177/030098589102800201
PMid:2063521

[3]. Deng, L.; Yang, M.; Fründ, S.; Wessel, T.; De Abreu, R. A.; Tischfield, J. A.; Sahota, J. A. Mol. Genet. Metab. 2001, 72, 260-264.
http://dx.doi.org/10.1006/mgme.2000.3142
PMid:11243733

[4]. Jacomelli, G.; Micheli, V.; Peruzzi, L.; Notarantonio, L.; Cerboni, B.; Sestini, S.; Pompucci, G. Clin. Chim. Acta 2002, 324, 135-139.
http://dx.doi.org/10.1016/S0009-8981(02)00243-7

[5]. Safranow, K.; Machoy, Z. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2005, 819(2), 229-235.
http://dx.doi.org/10.1016/j.jchromb.2004.11.013
PMid:15833286

[6]. Kathiwala, M.; Abou El-Nour, K. M.; Cohen-Shohet, R.; Brajter-Toth, A. Analyst 2010, 135, 296-301.
http://dx.doi.org/10.1039/b911649d
PMid:20098761

[7]. Kathiwala, M.; Affum, A. O.; Perry, J.; Brajter-Toth, A. Analyst 2008, 133, 810-816.
http://dx.doi.org/10.1039/b718125f
PMid:18493684

[8]. Bravo, R.; Hsueh, C. C.; Jamarillo, A.; Brajter-Toth, A. Analyst 1998, 123, 1625-1630.
http://dx.doi.org/10.1039/a802341g

[9]. Brajter-Toth, A.; Abou El-Nour, K.; Cavalheiro, E. T.; Bravo, R. Anal. Chem. 2000, 72, 1576-1584.
http://dx.doi.org/10.1021/ac9906680
PMid:10763255

[10]. Abou El-Nour, K.; Brajter-Toth, A. Electroanalysis 2000, 12, 805-810.
http://dx.doi.org/10.1002/1521-4109(200007)12:11<805::AID-ELAN805>3.0.CO;2-X

[11]. Hsueh, C. C.; Brajter-Toth, A. Anal. Chim. Acta 1996, 321, 209-214.
http://dx.doi.org/10.1016/0003-2670(95)00585-4

[12]. Bard, A. J. The Encyclopedia of The electrochemistry of The Elements, Marcel Dekker, 1973.

[13]. Bard, A. J.; Faulkner, R. L. Elechochemical Methods: Fundamentals and Application, Wiley, 1980.

[14]. Green, S. J.; Stokes, J. J.; Hostetler, M. J.; Pietron, J.; Murray, R. W. J. Phys. Chem. B.1997, 101(14), 2663-2668.
http://dx.doi.org/10.1021/jp962664u

[15]. Bravo, R.; Brajter-Toth, A. Chem. Anal. (Warsaw) 1999, 44, 423-436.

[16]. Hsueh, C. C.; Bravo, R.; Jaramillo, A.; Brajter-Toth, A. Anal. Chim. Acta 1997, 349, 67-76.
http://dx.doi.org/10.1016/S0003-2670(97)00209-2

[17]. McCreery, L. R. Electroanalytical Chemishy, in: Bard, A. J. (Ed.), Marcel Dekker, 1991, pp. 221-225.

[18]. Anjo, D. M.; Kahr, M.; Khodabakhsh, M. M.; Nowinski, S.; Wagner, M. Anal. Chem. 1989, 61, 2603-2608.
http://dx.doi.org/10.1021/ac00198a004

[19]. Kinoshita, K. Carbon: Electrochemical and Physical Properties, Wiley & Son, 1988.

[20]. Falat, L.; Cheng, H. Y. J. Electroanal. Chem. 1983, 157, 393-397.
http://dx.doi.org/10.1016/0022-0728(83)80344-1

[21]. Diculescu, V. C.; Piedade, J. A. P.; Oliveira-Brett, A. M. Bioelechemistry 2007, 70, 141-146.
http://dx.doi.org/10.1016/j.bioelechem.2006.03.015
PMid:16713382

[22]. Goyal, R. N.; Mittal, A. Indian J. Chem. 1993, 32A, 852-856.

[23]. Goyal, R. N.; Sangal, A. J. Electroanal. Chem. 2003, 557, 147-155.
http://dx.doi.org/10.1016/S0022-0728(03)00367-X

[24]. Goyal, R. N.; Sangal, A. J. Electroanal. Chem. 2002, 521, 72-80.
http://dx.doi.org/10.1016/S0022-0728(02)00645-9

[25]. Subramanian, P.; Dryhurst, G. J. Electroanal. Chem. 1987, 224, 137-162.
http://dx.doi.org/10.1016/0022-0728(87)85089-1

[26]. Oliveira-Brett, A. M.; Diculescu, V. C.; Piedade, J. A. P. Bioelechemistry 2002, 55, 61-62.
http://dx.doi.org/10.1016/S1567-5394(01)00147-5

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Egyptian Higher Education Ministry and University of Suez Canal, Ismailia, 41522, Egypt
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