European Journal of Chemistry 2013, 4(2), 162-167. doi:10.5155/eurjchem.4.2.162-167.753

Electrochemically activated carbon fiber sensors as fast and sensitive adenine metabolite amperometric detectors


Kholoud Mohammed Abou El-Nour (1,*)

(1) Department of Chemistry, University of Florida, Gainesville, Florida, 32611-7200, USA
(*) Corresponding Author

Received: 18 Feb 2013, Accepted: 25 Mar 2013, Published: 30 Jun 2013

Abstract


A direct and fast voltammetric method of determination of 2,8-dihydroxyadenine (2,8-DHA), with a novel nanostructured carbon fiber sensor (CFS), is revealed in this work. To achieve high sensitivity required in biological determinations, before the determination of 2,8-DHA, carbon fiber sensors were activated by electrochemical pretreatment method. The electrochemical activation process generates a nanostructured carbon fiber surface, which is stable. The high stability of the active surface, and the high reproducibility of the activation process, leads to reproducible and sensitive fast scan voltammetric (FSV) determinations of 2,8-DHA. The 2,8-DHA sensitivity was determined in 31 mM phosphate buffer and Hank’s Balanced Salt solution (HBSS) : phosphate buffer (1:5) pH = 7.4, with different scan rates of 250, 500 and 1000 V/s. The potential window in the determinations was from -1.0 to 1.5 V (vs. SCE). A high sensitivity of the new method of 2,8-DHA determinations by FSV at 500 V/swas found to be 0.40±0.01 nA µM-1 (r2 = 0.998) from the calibration plot of 2,8-DHA, with a linear dynamic range from 1-20 µM, and a limit of detection of 1 µM under physiological conditions.

4_2_162_167

Keywords


Carbon fiber sensor; 2,8-Dihydroxyadenine; Fast scan voltammetry; Electrochemical biosensors; Electrochemically activated sensor; Nanostructured ultramicroelectrode

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DOI: 10.5155/eurjchem.4.2.162-167.753

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How to cite


El-Nour, K. Eur. J. Chem. 2013, 4(2), 162-167. doi:10.5155/eurjchem.4.2.162-167.753
El-Nour, K. Electrochemically activated carbon fiber sensors as fast and sensitive adenine metabolite amperometric detectors. Eur. J. Chem. 2013, 4(2), 162-167. doi:10.5155/eurjchem.4.2.162-167.753
El-Nour, K. (2013). Electrochemically activated carbon fiber sensors as fast and sensitive adenine metabolite amperometric detectors. European Journal of Chemistry, 4(2), 162-167. doi:10.5155/eurjchem.4.2.162-167.753
El-Nour, Kholoud. "Electrochemically activated carbon fiber sensors as fast and sensitive adenine metabolite amperometric detectors." European Journal of Chemistry [Online], 4.2 (2013): 162-167. Web. 16 Sep. 2019
El-Nour, Kholoud. "Electrochemically activated carbon fiber sensors as fast and sensitive adenine metabolite amperometric detectors" European Journal of Chemistry [Online], Volume 4 Number 2 (30 June 2013)

DOI Link: https://doi.org/10.5155/eurjchem.4.2.162-167.753

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