European Journal of Chemistry 2014, 5(1), 65-72 | doi: https://doi.org/10.5155/eurjchem.5.1.65-72.903 | Get rights and content

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Hypoglycemic effect of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits in diabetic rat


Hemdan Ibrahem Mahmoud (1,*) , Said Mnaa Gad ElRab (2) , Ayman Fathey Khalil (3) , Samah Mohamed Ismael (4)

(1) Agricultural Chemistry Department, Faculty of Agriculture, Minia University, 61517, Minia, Egypt
(2) Home Economics Department, Faculty of Specific Education, Menofia University, 23811, Ashmoon, Menofia, Egypt
(3) Home Economics Department, Faculty of Specific Education, Ain Shams University, 11566, Cairo, Egypt
(4) Home Economics Department, Faculty of Specific Education, Ain Shams University, 11566, Cairo, Egypt
(*) Corresponding Author

Received: 11 Aug 2013 | Revised: 12 Sep 2013 | Accepted: 15 Sep 2013 | Published: 31 Mar 2014 | Issue Date: March 2014

Abstract


The aim of the present study was to investigate the hypoglycemic effect of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits either used individually or in a combination on alloxan diabetic rats. These fruits are reported to be rich in antioxidants, flavonoids and phenolics that can potentially fight against diabetes mellitus. Male albino rats were divided into 5 groups: normal control, alloxan-diabetic control, diabetic rats treated with white mulberry fruit powder at 5% in the diet, diabetic rats treated with black mulberry fruit powder at 5% in the diet and diabetic rats treated with mixture of white and black mulberry fruits powder at 5% in the diet. After 4 weeks of treatment, blood glucose level, liver and kidney enzymes activity, lipid profile, lipid peroxidation and histopathological studies on liver, kidney and pancreas were evaluated. The mixture of white and black mulberry fruits showed the most significant (p < 0.05) improvement in feed efficiency ratio with increasing body weight gain, as well as decrease in blood glucose level and liver-kidney dysfunction when compared with diabetic control rats. Significant decrease in serum cholesterol, triglycerides and low density lipoprotein cholesterol (LDLc) as well as significant increase in high density lipoprotein cholesterol (HDLc) in diabetic rats was observed with all treatments. Moreover, mulberry fruits administration caused significant inhibition in lipid peroxidation and α-amylase activity. In addition, the beneficial effect of all treatments was further confirmed with histopathological examination of liver, kidney and pancreas. This study reveals hypoglycemic and hypolipidemic effects of white and black mulberry fruits either used individually or in combination as a dietary supplement in alloxan diabetic rats.


Keywords


Alloxan; Antidiabetic; Hypoglycemic; Morus alba fruits; Morus nigra fruits; Lipid peroxidation

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DOI: 10.5155/eurjchem.5.1.65-72.903

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/


[2]. Alaa M. Abu-Odeh, Wamidh H. Talib
Middle East Medicinal Plants in the Treatment of Diabetes: A Review
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[3]. Dahae Lee, Ki Hyun Kim, Tae Su Jang, Ki Sung Kang
Identification of bioactive compounds from mulberry enhancing glucose-stimulated insulin secretion
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DOI: 10.1111/jphp.13338
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[6]. Yong Zhao, Kenneth Thermann Kongstad, Anna Katharina Jäger, John Nielsen, Dan Staerk
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DOI: 10.1016/j.chroma.2018.04.041
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[7]. Chun Chen, Bin Zhang, Xiong Fu, Rui Hai Liu
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[8]. Fadia S. Youssef, Rola M. Labib, Omayma A. Eldahshan, Abdel Nasser B. Singab
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DOI: 10.1002/cbdv.201700206
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DOI: 10.1002/ptr.7056
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References


[1]. Danaei, G.; Finucane, M. M.; Lu, Y.; Singh, G. M.; Cowan, M. J.; Paciorek, C. J. Lin, J. K. Farzadfar, F.; Khang, Y. H.; Stevens, G. A.; Rao, M.; Ali, M. K.; Riley, L. M.; Robinson, C. A.; Ezzati, M. Lancet 2011, 378, 31-40.
http://dx.doi.org/10.1016/S0140-6736(11)60679-X

[2]. Herman, W. H.; Aubert, R. E.; Ali, M. A.; Sous, E. S.; Badran, A. East Mediterr. Health. J. 1997, 3, 144-148.

[3]. Bliss, M. The Discovery of Insulin. University of Chicago Press, Chicago, USA, 2000.

[4]. Gillies, C. L.; Abrams, K. R.; Lambert, P. C.; Cooper, N. J.; Sutton, A. J.; Hsu, R. T. BMJ 2007, 334(7588), 299-307.
http://dx.doi.org/10.1136/bmj.39063.689375.55

[5]. Rabbani, S. I.; Devi, K.; Khanam, S. Indian J. Exp. Biol. 2009, 47, 804-810.

[6]. Kar, A. B; Chaudhry, K.; Bandyopadhyay, N. C. J Ethnopharmacol. 2003, 24, 105-108.
http://dx.doi.org/10.1016/S0378-8741(02)00144-7

[7]. Jayakar, B.; Rajkapoor, S. B. J. Herb. Pharmacother. 2004, 4(1), 35-40.

[8]. Schiller, J. S.; Bernadel, L. Vital Health Stat. 2004, 3, 10-48.

[9]. Hasler, C. M.; Bloch, A. S.; Thomson, C. A.; Enrione, E.; Manning, C. J. Am. Diet. Assoc. 2004, 104(5), 814-826.
http://dx.doi.org/10.1016/j.jada.2004.03.015

[10]. Millen, B. E.; Quatromoni, P. A.; Pencia, M.; Kimokoti, R.; Mam, B.; Cobain, S. et al. J. Am. Diet. Assoc. 2005, 105, 1732-1734.
http://dx.doi.org/10.1016/j.jada.2005.08.007

[11]. Bazanno, L. A.; He, J.; Ogden, L. G.; Loria, C. M.; Vupputuri. S.; Myers, L.; Whelton, P. K. Am. J. Clin. Nutr. 2002, 76, 93-99.

[12]. Abd EI-Ghany, M. A. Egypt. J. Nutr. 2002, 17(3), 227-244.

[13]. Huang, T. H.; Kota, B. P.; Razmovski, V.; Roufogalis, B. D. Basic Clin. Pharmacol. Toxicol. 2005, 96, 3-14.
http://dx.doi.org/10.1111/j.1742-7843.2005.pto960102.x

[14]. Ning, D. W.; Lu, B.; Zhang, Y. L. China Fruit Veg. Proc. 2005, 5, 38-40.

[15]. Wattanathorn, J.; Muchimapura, S.; Thukhammee, W.; Tong-un, T.; Wannanon, P.; Nattaporn, P Pratchaya, K.; Thanes, C.; Wiroje, K.; Suttisan P.; Sathaporn, W. Am. J. Applied Sci. 2012, 9, 1503-1511.

[16]. Ma, Y. P. Xinjiang J. Trad. Chin. Med. 2002, 20, 83-84.

[17]. Gong, S. X.; Zhu, J. P. J. Zhejiang Univ. Trad. Chin. Med. 2008, 32, 350-352.

[18]. Shizhen, L. Compendium of Materia Medica. 1st ed. Foreign Language Press, ISBN-10, 7119032607, 2008.

[19]. Hamzaa, R. G.; El Shahat A. N.; Mekawey, H. M. S. Biochem. Anal. Biochem. 2012, 1(122), 1-8, doi:10. 4172/2161-1009. 1000122

[20]. Kojima, Y.; Kimura, T.; Nakagawa, K.; Asai, A.; Hasumi, K.; Oikawa, S. Miyazawa, T. J. Clin. Biochem. Nutr. 2010, 47, 155-161.
http://dx.doi.org/10.3164/jcbn.10-53

[21]. Kwon, H. J.; Chung, J. Y.; Kim, J. Y.; Kwon, O. J. Agric. Food Chem. 2011, 59, 3014-3019.
http://dx.doi.org/10.1021/jf103463f

[22]. Kostic, D. A.; Dimitrijevic, D. S.; Mitic, S. S.; Mitic, M. N., Stojanovic, G. S.; Zivanovic, A. V. Trop. J. Pharm. Res. 2013, 12(1), 105-110.

[23]. Naderi, G. A.; Asgary, S.; Sarraf-Zadegan, N.; Oroojy, H.; Afshin-Nia, F. Phytother. Res. 2004, 18, 365-369.
http://dx.doi.org/10.1002/ptr.1400

[24]. Hansawasdi, C.; Kawabata, J. Fitoterapia 2006, 77, 568-573.
http://dx.doi.org/10.1016/j.fitote.2006.09.003

[25]. Kaewkaen, P.; Tong-Un, T.; Wattanathorn, J.; Muchimapura, S.; Kaewrueng, W.; Wongcharoenwanakit, S. Am. J. Agric. Biol. Sci. 2012, 7 (3), 322-329.

[26]. Tsantili, E.; Shin, Y.; Nock, J. F.; Watkins, C. B. Postharvest Biol. Tec. 2010, 57, 27-34.
http://dx.doi.org/10.1016/j.postharvbio.2010.02.002

[27]. Chang, C. C.; Yang, M. H.; Wen, H. M.; Chern, J. C. J. Food Drug Anal. 2002, 10, 178-182.

[28]. Jakobek, L.; Seruga, M.; Medvidovic-Kosanovi, M.; Novak, I. Agric. Conspec. Sci. 2007, 72(4), 301-306.

[29]. Sheweita, A. A.; Newairy, H. A.; Mansour, M. I. Toxicology 2002, 174, 131-139.
http://dx.doi.org/10.1016/S0300-483X(02)00048-3

[30]. Zhang, J.; Huang, Y.; Hou, T.; Wang, Y. Swiss Med. Wkly. 2006, 136, 529-532.

[31]. Yang, X.; Yang, L.; Zheng, H. Food Chem. Toxicol. 2010, 48(8-9), 2374-2379.
http://dx.doi.org/10.1016/j.fct.2010.05.074

[32]. Trinder, P. Ann. Clin. Biochem. 1969, 6, 24-27.

[33]. Fossati, P.; Prencipe, L. Clin. Chem. 1982, 28, 2077-2080.

[34]. Richmond, W. Clin. Chem. 1973, 19, 1350-1356.

[35]. Lopes-Virella, M. F.; Stone, P.; Ellis, S.; Colwell, J. A. Clin. Chem. 1977, 23, 882-884.

[36]. Friedewald, W.; Levy, R.; Fredrickson, D. Clin. Chem. 1972, 18, 499-502.

[37]. Reitman, S.; Frankel, S. Am. J. Clin. Path. 1957, 28, 56-63.

[38]. Fawcett, J. K.; Scott, J. E. J. Clin. Path. 1960, 13, 156-159.
http://dx.doi.org/10.1136/jcp.13.2.156

[39]. Larsen, K. Clin. Chim. Acta 1972, 41, 209-217.
http://dx.doi.org/10.1016/0009-8981(72)90513-X

[40]. Ohkawa, H.; Ohishi, W.; Yagi, K. Anal. Biochem. 1979, 95(2), 351-358.
http://dx.doi.org/10.1016/0003-2697(79)90738-3

[41]. Rinderknecht, H.; Wilding, P.; Haverback, B. J. Experientia 1967, 23(10), 805-805.
http://dx.doi.org/10.1007/BF02146851

[42]. Banchroft, J.; Stevens, A.; Turner, D. Theory and practice of histological techniques. 4th ed. Churchil Livingstone, New York, London, San Francisco, Tokyo, 1996.

[43]. SPSS. Statistical Package for Social Science. Computer Software, Ver. 10. SPSS Company: London, UK, 1998.

[44]. Butkhup, L.; Samappito, W.; Samappito, S. Int. J. Food Sci. Tech. 2013, 48(5), 934-940.
http://dx.doi.org/10.1111/ijfs.12044

[45]. Ercisli, S.; Orhan, E. Food Chem. 2007, 103(4), 1380-1384.
http://dx.doi.org/10.1016/j.foodchem.2006.10.054

[46]. Zadernowski, R.; Naczk, M.; Nesterowicz, J. J. Agric. Food Chem. 2005, 53 (6), 2118-2124.
http://dx.doi.org/10.1021/jf040411p

[47]. Andallu, B.; Varadacharyulu, N. C. Clin. Chim. Acta 2003, 338, 3-10.
http://dx.doi.org/10.1016/S0009-8981(03)00322-X

[48]. Szkudelski, T. Physiol. Res. 2001, 50 (6), 537-546.

[49]. Chatterjea, M. N.; Shinde, R. Text book of medical biochemistry. 5th ed. New Delhi, Jaypee Brothers, Medical Publishers (P) Ltd., 2002.

[50]. Singab, A. N.; El-Beshbishy, H. A.; Yonekawa, M.; Nomura, T.; Fukai, T. J. Ethnopharmacol. 2005, 100, 333-338.
http://dx.doi.org/10.1016/j.jep.2005.03.013

[51]. Ma, D. Q.; Jiang, Z. J.; Xu, S. Q.; Yu, X.; Hu, X. M.; Pan, H. Y. Chinese Herb. Med. 2012, 4(4), 314-318.

[52]. Grover, J. K.; Yadav, S.; Vats, V. J. Ethnopharmacol. 2002, 81(1), 81-100.
http://dx.doi.org/10.1016/S0378-8741(02)00059-4

[53]. Chen, F. J.; Nakashima, N.; Kimura, M. Yakugaku Zasshi. 1995, 115, 476-482.

[54]. Zhang, X. M.; Liang, W. B.; Mao, Y. Q.; Li, H.; Yang, Y.; Tan, H. R. Biomed. Pharmacother. 2009, 63, 180-186.
http://dx.doi.org/10.1016/j.biopha.2007.07.006

[55]. Wohaieb, S. A.; Godin D. Y. Diabetes 1987, 3, 1014-1021.
http://dx.doi.org/10.2337/diab.36.9.1014

[56]. Chaudary, A. R.; Alam, M.; Ahmad, M. Fitoterapia 1993, 64, 510-515.

[57]. Chaurasia, S.; Saxena, R. C.; Chaurasia, I. D.; Shrivastava, R. Int. J. Chem. Sci. 2011, 9(2), 489-492.

[58]. Hui-Yin, C.; Gow-Chin, Y. Food Chem. 2007, 101, 686-694.
http://dx.doi.org/10.1016/j.foodchem.2006.02.047

[59]. Jarald, E. E. Joshi, S. B.; Jain, D. C. Indian J. Exp. Biol. 2008, 46, 660-667.

[60]. Shou-Chieh, W.; Shiow-Fen, L.; Chau-Jong, W.; Chao-Hsin, L.; Wen-Chin, L.; Huei-Jane, L. eCAM. 2009, 4, 1-9.

[61]. Shah, S. V.; Baliga, R.; Rajapurkar, M.; Fonseca, V. A. J. Am. Soc. Nephrol. 2007, 18, 16-28.
http://dx.doi.org/10.1681/ASN.2006050500

[62]. Jain, S.; Bhatia, G.; Barik, R.; Kumar, P.; Jain, A.; Dixit, V. K. J. Ethnopharmacol. 2010, 127, 325-328.
http://dx.doi.org/10.1016/j.jep.2009.10.038

[63]. Andallu, B.; Vinay Kumar, A. V.; Varadacharyulu, N. C. Int. J. Diabetes Dev. Ctries. 2009, 29(3), 123-128.
http://dx.doi.org/10.4103/0973-3930.54289

[64]. Lebovit, H. Diabetes Rev. 1998, 6, 132-145.

[65]. McDougall, G. J.; Stewart, D. Biofactors 2005, 23(4), 189-195.
http://dx.doi.org/10.1002/biof.5520230403

[66]. Farber, J. L.; Kyle, M. E.; Coleman, J. B. Lab. Invest. 1990, 62, 670-679.

[67]. Hamzaa, R. G.; Mekawey, H. M. S.; El Shahat, A. N. Arab J. Nucl. Sci. Appl. 2013, 46 (2), 347-355.

[68]. Baynes, J. W. Diabetes 1991, 40, 405-412.
http://dx.doi.org/10.2337/diab.40.4.405

[69]. Oh, H.; Ko, E. K.; Jun, J. Y.; Oh, M. H.; Park, S. U.; Kang, K. H.; Lee, H. S.; Kim, Y. C. Plant Med. 2002, 68, 932-934.
http://dx.doi.org/10.1055/s-2002-34930

[70]. Coskun, O.; Kanter, M.; Korkmaz, A.; Oter, S. Pharmacol. Res. 2005, 51, 117-123.
http://dx.doi.org/10.1016/j.phrs.2004.06.002

[71]. Mohammadi, J.; Naik, P. R. Turk. J. Biol. 2012, 36, 211-216.


How to cite


Mahmoud, H.; ElRab, S.; Khalil, A.; Ismael, S. Eur. J. Chem. 2014, 5(1), 65-72. doi:10.5155/eurjchem.5.1.65-72.903
Mahmoud, H.; ElRab, S.; Khalil, A.; Ismael, S. Hypoglycemic effect of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits in diabetic rat. Eur. J. Chem. 2014, 5(1), 65-72. doi:10.5155/eurjchem.5.1.65-72.903
Mahmoud, H., ElRab, S., Khalil, A., & Ismael, S. (2014). Hypoglycemic effect of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits in diabetic rat. European Journal of Chemistry, 5(1), 65-72. doi:10.5155/eurjchem.5.1.65-72.903
Mahmoud, Hemdan, Said Mnaa Gad ElRab, Ayman Fathey Khalil, & Samah Mohamed Ismael. "Hypoglycemic effect of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits in diabetic rat." European Journal of Chemistry [Online], 5.1 (2014): 65-72. Web. 22 Mar. 2023
Mahmoud, Hemdan, ElRab, Said, Khalil, Ayman, AND Ismael, Samah. "Hypoglycemic effect of white (Morus alba L.) and black (Morus nigra L.) mulberry fruits in diabetic rat" European Journal of Chemistry [Online], Volume 5 Number 1 (31 March 2014)

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