European Journal of Chemistry 2014, 5(2), 241-246 | doi: https://doi.org/10.5155/eurjchem.5.2.241-246.1000 | Get rights and content

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A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma


Kareem Mahmoud Younes (1,*) , Ehab Farouk El-Kady (2)

(1) Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, ET-11562, Egypt
(2) Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, ET-11562, Egypt
(*) Corresponding Author

Received: 09 Dec 2013 | Revised: 26 Dec 2013 | Accepted: 26 Dec 2013 | Published: 30 Jun 2014 | Issue Date: June 2014

Abstract


A simple, rapid and sensitive LC-MS/MS method was developed and validated for the quantification of azithromycin (AZI) in human plasma. Sildenafil citrate was used as an internal standard. The analytes were extracted from human plasma samples by liquid-liquid extraction technique. The reconstituted samples were chromatographed on a reversed ‑ phase column C18, 50 × 2.1 mm, 5 µm from Waters, by using a 80:20 (v:v) mixture of acetonitrile and 0.1% formic acid in water as the mobile phase at a flow rate of 0.5 mL/min. The calibration curve was linear (r2 > 0.99) over the concentration range of 5.0-1500.0 ng/mL. The results of the intra- and inter-day precision and accuracy studies were within the acceptable limits. A run time of 0.5 min for each sample made it possible to analyze more than 400 plasma samples per day.


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Keywords


Azithromycin; Sildenafil citrate; Internal standard; Mass spectrometry; Liquid-liquid extraction; Liquid chromatography

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DOI: 10.5155/eurjchem.5.2.241-246.1000

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Funding information


The Holding Company for Biological Products and Vaccines (VACSERA), Cairo, Egypt

Citations

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[1]. Jelena Lović, Nemanja Trišović, Jelena Antanasijević, Nebojša D. Nikolić, Sanja Stevanović, Dušan Mijin, Dragan Vuković, Aleksandar Mladenović, Slobodan Petrović, Milka Avramov Ivić
Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode
Journal of Electroanalytical Chemistry  782, 103, 2016
DOI: 10.1016/j.jelechem.2016.10.022
/


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How to cite


Younes, K.; El-Kady, E. Eur. J. Chem. 2014, 5(2), 241-246. doi:10.5155/eurjchem.5.2.241-246.1000
Younes, K.; El-Kady, E. A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma. Eur. J. Chem. 2014, 5(2), 241-246. doi:10.5155/eurjchem.5.2.241-246.1000
Younes, K., & El-Kady, E. (2014). A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma. European Journal of Chemistry, 5(2), 241-246. doi:10.5155/eurjchem.5.2.241-246.1000
Younes, Kareem, & Ehab Farouk El-Kady. "A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma." European Journal of Chemistry [Online], 5.2 (2014): 241-246. Web. 26 Nov. 2022
Younes, Kareem, AND El-Kady, Ehab. "A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma" European Journal of Chemistry [Online], Volume 5 Number 2 (30 June 2014)

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