European Journal of Chemistry 2014, 5(2), 241-246. doi:10.5155/eurjchem.5.2.241-246.1000

A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma


Kareem Mahmoud Younes (1,*) , Ehab Farouk El-Kady (2)

(1) Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, ET-11562, Egypt
(2) Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, ET-11562, Egypt
(*) Corresponding Author

Received: 09 Dec 2013, Accepted: 26 Dec 2013, Published: 30 Jun 2014

Abstract


A simple, rapid and sensitive LC-MS/MS method was developed and validated for the quantification of azithromycin (AZI) in human plasma. Sildenafil citrate was used as an internal standard. The analytes were extracted from human plasma samples by liquid-liquid extraction technique. The reconstituted samples were chromatographed on a reversed ‑ phase column C18, 50 × 2.1 mm, 5 µm from Waters, by using a 80:20 (v:v) mixture of acetonitrile and 0.1% formic acid in water as the mobile phase at a flow rate of 0.5 mL/min. The calibration curve was linear (r2 > 0.99) over the concentration range of 5.0-1500.0 ng/mL. The results of the intra- and inter-day precision and accuracy studies were within the acceptable limits. A run time of 0.5 min for each sample made it possible to analyze more than 400 plasma samples per day.


Keywords


Azithromycin; Sildenafil citrate; Internal standard; Mass spectrometry; Liquid-liquid extraction; Liquid chromatography

Full Text:

PDF /    /


DOI: 10.5155/eurjchem.5.2.241-246.1000

Article Metrics


This Abstract was viewed 496 times | PDF Article downloaded 301 times

Citations

/


[1]. Jelena Lović, Nemanja Trišović, Jelena Antanasijević, Nebojša D. Nikolić, Sanja Stevanović, Dušan Mijin, Dragan Vuković, Aleksandar Mladenović, Slobodan Petrović, Milka Avramov Ivić
Electrochemical determination of sildenafil citrate as standard, in tablets and spiked with human serum at gold and cystein modified gold electrode
Journal of Electroanalytical Chemistry  782, 103, 2016
DOI: 10.1016/j.jelechem.2016.10.022
/


References

[1]. Reynolds, J. E. F. (Ed.), Martindale: The Extra Pharmacopoeia, 36th edition, the Pharmaceutical Press, London, 2009, 207.

[2]. Ghodsi, R.; Kobarfard, K.; Tabatabai S. A. Iranian J. Pharm. Res. 2012, 11, 123-127.

[3]. Suhagia, B. N.; Shah, S. A.; Rathod, I. S.; Patel, H. M.; Doshi, K. R. Indian J. Pharm. Sci. 2006, 68, 242-245.
http://dx.doi.org/10.4103/0250-474X.25726

[4]. Mallah, M. A.; Sherazi, S. T. H.; Mahesar, S. A.; Rauf, A. Pak. J. Anal. Environ. Chem. 2011, 12, 61-67.

[5]. Khashaba, P. Y. J. Pharm. Biomed. Anal. 2002, 27, 923-932.
http://dx.doi.org/10.1016/S0731-7085(01)00609-4

[6]. Sastre, T. J.; Guchelaar, H. J. J. Chromatogr B. 1998, 720, 89-97.
http://dx.doi.org/10.1016/S0378-4347(98)00456-3

[7]. Bahrami, G.; Mirzae, S.; Kiani, A. J. Chromatogr B. 2005, 820, 277-281.
http://dx.doi.org/10.1016/j.jchromb.2005.03.042

[8]. Bahrami, G.; Mohammadi, B. J. Chromatogr B. 2006, 830, 355-358.
http://dx.doi.org/10.1016/j.jchromb.2005.10.044

[9]. Taninaka, C.; Ohtani, H.; Hanada, E.; Kotaki, H.; Sato, H.; Iga, T. J. Chromatogr B. 2000, 738, 405-411.
http://dx.doi.org/10.1016/S0378-4347(99)00512-5

[10]. Palomeque, M. E.; Ortiz, P. I. Talanta 2007, 72, 101-105.
http://dx.doi.org/10.1016/j.talanta.2006.10.002

[11]. Kees, F.; Spangler, S.; Wellenhofer, M. J. Chromatogr A. 1998, 812, 287-293.
http://dx.doi.org/10.1016/S0021-9673(97)01279-X

[12]. Shepard, R. M.; Duthu, G. S.; Ferraina, R. A.; Mullins, M. A. J. Chromatogr. 1991, 565 (1-2), 321-337.
http://dx.doi.org/10.1016/0378-4347(91)80393-Q

[13]. Gandhi, R.; Kaul, C. L.; Panchagnula, R. J. Pharmaceut. Biomed. Anal. 2000, 23, 1073-1078.
http://dx.doi.org/10.1016/S0731-7085(00)00406-4

[14]. Fouda, G. H.; Schneider, R. P. Ther. Drug Monit. 1995, 17, 179-183.
http://dx.doi.org/10.1097/00007691-199504000-00013

[15]. Abuin, S. O.; Codony, R.; Ramon, N. O.; Granados, M. E.; Dolors, P. M. J. Chromatogr A. 2006, 1114(1), 73-81.
http://dx.doi.org/10.1016/j.chroma.2006.02.032

[16]. Koch, D. E.; Bhandari, A.; Close, L.; Robert, P. H. J. Chromatogr A. 2005, 1074, 17-22.
http://dx.doi.org/10.1016/j.chroma.2005.03.052

[17]. Shen, Y.; Yin, C.; Mengxiang, S. U.; Jiasheng, T. U. J. Pharmaceut. Biomed. Anal. 2010, 52, 99-104.
http://dx.doi.org/10.1016/j.jpba.2009.12.001

[18]. Rockville, M. D. U. S. Pharmacopeia 35, National Formulary 30. The United States Pharmacopeial Commission Inc. 2010, 1965-1973.

[19]. Miguel, L.; Barbas, C. J. Pharm. Biomed. Anal. 2003, 33, 211-217.
http://dx.doi.org/10.1016/S0731-7085(03)00258-9

[20]. Patricia, Z.; Rita, C.; Maria, A. R.; Maria, T. P. J. Pharmaceut. Biomed. Anal. 2002, 27, 833-836.
http://dx.doi.org/10.1016/S0731-7085(01)00554-4

[21]. Tayebeh, G.; Farzad, K.; Seyed, A. M. Iranian J. Pharm. Res. 2013, 12, 57-63.

[22]. Hashemi, M. S. H.; Kobarfard, F.; Husain, S. W.; Tehrani, M. S.; Abroomand, A. P.; Ahmadkhanihac, R.; Mehdizadeh, A. Iranian J. Pharm. Res. 2012, 11, 59-67.

[23]. Fei, L.; Yu, X.; Jinchang, H.; Shu, G.; Qingxiang, G. Biomed Chromatogr. 2007, 21(12), 1272-1278.
http://dx.doi.org/10.1002/bmc.883

[24]. Xue-Min, Z.; Jie, L.; Juan, G.; Quan-Sheng, Y.; Wen-Yan, W. Pharmazie 2007, 62, 255-257.

[25]. Gobela, A.; Thomsena, A.; McArdella, C.; Aldera, A.; Gigera, W.; Theibb, N.; Lofflerb, D.; Ternes, T. J. Chromatog. A 2005, 1085(2), 179-189.
http://dx.doi.org/10.1016/j.chroma.2005.05.051

[26]. Chena, B.; Liangb, Y.; Chenc, X.; Liua, S.; Denga, F.; Zhoua, P. J. Pharm. Biomed. Anal. 2006, 42(4), 480-487.
http://dx.doi.org/10.1016/j.jpba.2006.05.011

[27]. Xu, F.; Zhang, Z.; Bian, Z.; Tian, Y.; Jiao, H.; Liu, Y. J. Chromatogr. Sci. 2008, 46, 479-484.
http://dx.doi.org/10.1093/chromsci/46.6.479

[28]. Supattanapong, S.; Konsil, J. Southeast Asian J. Trop. Med. Public Health 2008, 39(6), 979-987.

[29]. Hidy, B. J.; Lewis, J.; Ke, J. Proceedings of 50th ASMS Conference on Mass Spectrometry and Applied Topics, Orlando, Florida, 2002.

[30]. U.S. Department of Health and Human Services, Food and Drug Administration (US FDA). Guidance for Industry, Bioanalytical Method Validation. FDA/CDER/CVM, Federal Register, 2001, Available from: URL: http://www.fda.gov/cder/guidance/4252fnl.pdf


How to cite


Younes, K.; El-Kady, E. Eur. J. Chem. 2014, 5(2), 241-246. doi:10.5155/eurjchem.5.2.241-246.1000
Younes, K.; El-Kady, E. A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma. Eur. J. Chem. 2014, 5(2), 241-246. doi:10.5155/eurjchem.5.2.241-246.1000
Younes, K., & El-Kady, E. (2014). A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma. European Journal of Chemistry, 5(2), 241-246. doi:10.5155/eurjchem.5.2.241-246.1000
Younes, Kareem, & Ehab Farouk El-Kady. "A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma." European Journal of Chemistry [Online], 5.2 (2014): 241-246. Web. 21 Oct. 2019
Younes, Kareem, AND El-Kady, Ehab. "A new validated bio-analytical liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the quantification of Azithromycin in human plasma" European Journal of Chemistry [Online], Volume 5 Number 2 (30 June 2014)

DOI Link: https://doi.org/10.5155/eurjchem.5.2.241-246.1000

Refbacks

  • There are currently no refbacks.




Copyright (c)




© Copyright 2019  Atlanta Publishing House LLC All Right Reserved.

The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.

Copyright 2019 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 4614 Lavista road, Tucker, GA, 30084, USA. Registered in USA.