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Fatty acid, tocopherol, mineral composition, total phenolic, flavonoid and thymoquinone content, and antioxidant potential of Nigella stellaris
Sibel Silahtaroglu (1)
, Serife Selma Uras Güngör (2) , Ahmet Ilçim (3) , Gamze Kökdil (4,*) (1) Department of Pharmacognosy, Faculty of Pharmacy, Mersin University, Yenisehir Campus, 33169 Mersin, Turkey
(2) Department of Pharmacognosy, Faculty of Pharmacy, Mersin University, Yenisehir Campus, 33169 Mersin, Turkey
(3) Department of Biology, Faculty of Arts and Science, Mustafa Kemal University, 31000, Antakya, Hatay, Turkey
(4) Department of Pharmacognosy, Faculty of Pharmacy, Mersin University, Yenisehir Campus, 33169 Mersin, Turkey
(*) Corresponding Author
Received: 26 Dec 2013 | Revised: 05 Jan 2014 | Accepted: 05 Jan 2014 | Published: 30 Jun 2014 | Issue Date: June 2014
Abstract
In the present study, the fatty acid composition of the fixed oil from seeds of Nigella stellaris has been investigated by GC-MS. Linoleic and oleic acids were the major fatty acids. The tocopherols determined by HPLC and α- and δ-tocopherols were detected in the oil. The seeds also were analyzed by ICP-MS to determine mineral content. Ca, P, and K were main elements among seventeen minerals in the seeds. Total phenolic and flavonoid contents of the aqueous methanolic extracts of different plant parts were assessed by Folin-Ciocalteu method and the AlCl3 assay. The seed extract was also analyzed by RP-HPLC analysis for its thymoquinone content. The present study showed that the seed extract contained low amount of thymoquinone. The seed extract exhibited the significantly higher total phenolic and flavonoid content than the extract of aerial part. DPPH radical scavenging activity was used to evaluate the antioxidant capacity of the extracts. Both of the extracts showed high antioxidant activity.
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European Journal of Chemistry
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DOI: 10.5155/eurjchem.5.2.263-266.1006
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University of Mersin (Project number: BAP-SBE-EMB (SS) 2008-6 YL), Mersin, Turkey
Citations
[1]. Hany Salah Mahmoud, Amani A. Almallah, Heba Nageh Gad EL-Hak, Tahany Saleh Aldayel, Heba M. A. Abdelrazek, Howayda E. Khaled
The effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats
Scientific Reports 11(1), , 2021
DOI: 10.1038/s41598-021-86721-1
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DOI Link: https://doi.org/10.5155/eurjchem.5.2.263-266.1006
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European Journal of Chemistry 2014, 5(2), 263-266 | doi: https://doi.org/10.5155/eurjchem.5.2.263-266.1006 | Get rights and content
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