European Journal of Chemistry 2015, 6(4), 468-474. doi:10.5155/eurjchem.6.4.468-474.1322

Simultaneous determination of paracetamol, caffeine and codeine in tablets and human plasma by micellar liquid chromatography


Fathalla Belal (1) , Mahmoud Ahmed Omar (2) , Sayed Derayea (3) , Sahar Zayed (4,*) , Mohamed Abdelkhalek Hammad (5) , Safaa Fathy Saleh (6)

(1) Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, 35516, Egypt
(2) Department of Analytical Chemistry, Faculty of Pharmacy, University of Minia, Minia, 61519, Egypt
(3) Department of Analytical Chemistry, Faculty of Pharmacy, University of Minia, Minia, 61519, Egypt
(4) Unit of Drug Analysis, Faculty of Pharmacy, University of Mansoura, Mansoura, 35516, Egypt
(5) Department of Analytical Chemistry, Faculty of Pharmacy, University of Minia, Minia, 61519, Egypt
(6) Department of Analytical Chemistry, Faculty of Pharmacy, University of Minia, Minia, 61519, Egypt
(*) Corresponding Author

Received: 14 Sep 2015, Accepted: 10 Oct 2015, Published: 31 Dec 2015

Abstract


A simple, rapid, sensitive and eco-friendly liquid chromatographic method was developed and validated for the simultaneous determination of paracetamol (PAR), caffeine (CAF) and codeine (COD). The separation was performed on cyano column using a micellar mobile phase consists of 140 mM sodium dodecyl sulfate, 25 mM phosphate buffer and 10% acetonitrile at pH = 3. The analysis was performed at a flow rate of 1 mL/min and a column temperature of 30 °C under direct UV detection at 210 nm. Total analysis time was below 6 min. Baclofen (BCF) was used as an internal standard. The validation was performed according to the ICH guidelines. The proposed method was linear over the ranges of 0.2-100.0, 0.02-12.0 and 0.2-12.0 µg/mL for PAR, CAF and COD, respectively. The limits of detection were 0.031, 0.007 and 0.054 µg/mL and limits of quantification 0.103, 0.02 and 0.164 µg/mL for PAR, CAF and COD, respectively. The results show that the procedure is suitable for the routine analysis of drugs in tablet dosage forms. The method was further extended to the determination of the studied drugs in spiked human plasma with mean percentage recoveries of 99.61±0.530, 99.28±0.523 and 99.52±0.385 for PAR, CAF and COD, respectively.


Keywords


Tablets; Codeine; Caffeine; Paracetamol; Human plasma; Micellar liquid chromatography

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DOI: 10.5155/eurjchem.6.4.468-474.1322

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Citations

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[1]. F. Belal, F. Ibrahim, Z.A. Sheribah, H. Alaa
Micellar HPLC method for the simultaneous determination of three anticonvulsant drugs in dosage forms and biological fluids. Application to dissolution-rate testing
Annales Pharmaceutiques Françaises  76(3), 172, 2018
DOI: 10.1016/j.pharma.2018.02.003
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[2]. Eman I El-Kimary, Essam F Khamis, Saeid F Belal, Mona M Abdel Moneim
Robust Chromatographic Methods for the Analysis of Two Quaternary Mixtures Containing Paracetamol, Codeine, Guaifenesin and Pseudoephedrine or Phenylephrine in their Dosage Forms
Journal of Chromatographic Science  , , 2019
DOI: 10.1093/chromsci/bmz057
/


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How to cite


Belal, F.; Omar, M.; Derayea, S.; Zayed, S.; Hammad, M.; Saleh, S. Eur. J. Chem. 2015, 6(4), 468-474. doi:10.5155/eurjchem.6.4.468-474.1322
Belal, F.; Omar, M.; Derayea, S.; Zayed, S.; Hammad, M.; Saleh, S. Simultaneous determination of paracetamol, caffeine and codeine in tablets and human plasma by micellar liquid chromatography. Eur. J. Chem. 2015, 6(4), 468-474. doi:10.5155/eurjchem.6.4.468-474.1322
Belal, F., Omar, M., Derayea, S., Zayed, S., Hammad, M., & Saleh, S. (2015). Simultaneous determination of paracetamol, caffeine and codeine in tablets and human plasma by micellar liquid chromatography. European Journal of Chemistry, 6(4), 468-474. doi:10.5155/eurjchem.6.4.468-474.1322
Belal, Fathalla, Mahmoud Ahmed Omar, Sayed Derayea, Sahar Zayed, Mohamed Abdelkhalek Hammad, & Safaa Fathy Saleh. "Simultaneous determination of paracetamol, caffeine and codeine in tablets and human plasma by micellar liquid chromatography." European Journal of Chemistry [Online], 6.4 (2015): 468-474. Web. 17 Sep. 2019
Belal, Fathalla, Omar, Mahmoud, Derayea, Sayed, Zayed, Sahar, Hammad, Mohamed, AND Saleh, Safaa. "Simultaneous determination of paracetamol, caffeine and codeine in tablets and human plasma by micellar liquid chromatography" European Journal of Chemistry [Online], Volume 6 Number 4 (31 December 2015)

DOI Link: https://doi.org/10.5155/eurjchem.6.4.468-474.1322

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