European Journal of Chemistry 2016, 7(2), 238-242. doi:10.5155/eurjchem.7.2.238-242.1433

Novel contribution in online sample cleanup for quantitative determination of Levetiracetam in human plasma by LC-Tandem mass spectrometry


Ahmed Abdalazim Mostafa (1,*)

(1) Pharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, EinHelwan, Cairo, 11795, Egypt
(*) Corresponding Author

Received: 02 Apr 2016, Accepted: 23 Apr 2016, Published: 30 Jun 2016

Abstract


The aim of this work was to develop and validate a simple, sensitive and rapid method for the quantitation of levetiracetam (LEV) in plasma using LC-Tandem mass spectrometry. Plasma samples were prepared by simple protein precipitation using acetonitrile; atenolol was used as internal standard (IS). Chromatographic separation was done on Luna 5 µm C18(2) (Phenomenex) 50 × 2.0 mm using gradient flow using solvent A (0.1% formic acid) and solvent B (0.1% formic acid in acetonitrile: water, 95:5, v:v). The positive-ion mass spectrometric detection method utilized electrospray ionization and the multiple reaction monitoring (MRM) mode. The MRM ion transitions were170.9 → 140.8 and 267.3 → 145.1 for levetiracetam and atenolol, respectively. The retention time of levetiracetam and atenolol is 3.24 and 2.96 min, respectively. The total run time was 5 min. The assay was validated over a concentration range from 1 to 100 µg/mL. The method was robust (minimal matrix effect), sensitive (LOQ, 1 µg/mL) metabolites and reproducible (The precision and accuracy for both intra- and inter-day were acceptable <15%). The method can be done on traditional LC-MS equipment. The method was effectively applied to single case study receiving toxic dose of LEV.


Keywords


MRM; Epilepsy; LC-MS/MS; Guard column; Tandem mass; Online sample cleanup

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DOI: 10.5155/eurjchem.7.2.238-242.1433

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How to cite


Mostafa, A. Eur. J. Chem. 2016, 7(2), 238-242. doi:10.5155/eurjchem.7.2.238-242.1433
Mostafa, A. Novel contribution in online sample cleanup for quantitative determination of Levetiracetam in human plasma by LC-Tandem mass spectrometry. Eur. J. Chem. 2016, 7(2), 238-242. doi:10.5155/eurjchem.7.2.238-242.1433
Mostafa, A. (2016). Novel contribution in online sample cleanup for quantitative determination of Levetiracetam in human plasma by LC-Tandem mass spectrometry. European Journal of Chemistry, 7(2), 238-242. doi:10.5155/eurjchem.7.2.238-242.1433
Mostafa, Ahmed. "Novel contribution in online sample cleanup for quantitative determination of Levetiracetam in human plasma by LC-Tandem mass spectrometry." European Journal of Chemistry [Online], 7.2 (2016): 238-242. Web. 17 Sep. 2019
Mostafa, Ahmed. "Novel contribution in online sample cleanup for quantitative determination of Levetiracetam in human plasma by LC-Tandem mass spectrometry" European Journal of Chemistry [Online], Volume 7 Number 2 (30 June 2016)

DOI Link: https://doi.org/10.5155/eurjchem.7.2.238-242.1433

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