European Journal of Chemistry

Development and validation of stability indicating HPLC method for quantification of tinidazole

Crossmark


Main Article Content

Randa Bakheet Ahmed
Mohamed El-Muktar Abdelaziz
Ahmed Elsadig Mohammed Saeed

Abstract

A simple, precise, reproducible and economical HPLC method for estimation of tinidazole has been developed. The wavelength of 317 nm is selected as λmax for tinidazole in phosphate buffer (pH = 6.8). Validation parameters were tested following International Conference on Harmonization (ICH) guideline. Tinidazole shows linearity at the selected wavelength and obeys Beer’s law in the concentration range of 3.2-40.0 µg/mL with correlation coefficient of 0.9999. Recovery studies for tinidazole were performed and the percentage recovery was obtained in the range of 99.10-102.45% confirming the accuracy of the proposed method. The method showed good reproducibility and recovery with %RSD less than 2. Statistical validation of the data shows that the proposed method can be used as stability indicating method which successfully applied for the routine analysis of drug in commercial tablets.


icon graph This Abstract was viewed 1667 times | icon graph Article PDF downloaded 625 times

How to Cite
(1)
Ahmed, R. B.; Abdelaziz, M. E.-M.; Saeed, A. E. M. Development and Validation of Stability Indicating HPLC Method for Quantification of Tinidazole. Eur. J. Chem. 2019, 10, 102-107.

Article Details

Share
Crossref - Scopus - Google - European PMC
References

[1]. Nilsson-Ehle, I.; Ursing, B.; Nilsson-Ehle, P. Antimicrob Agents Chemother. 1981, 19, 754-760.
https://doi.org/10.1128/AAC.19.5.754

[2]. Jiang, X. Y.; Chen, X. Q.; Dong, Z.; Xu, M. Autom. Methods Manag. Chem. 2007, 2007, 86857-86864.
https://doi.org/10.1155/2007/86857

[3]. Rajnarayana, K.; Chaluvadi, M. R.; Alapati, V. R.; Mada, S. R.; Jayasagar, G.; Krishna, D. R. Pharmazie 2002, 57, 535-542.

[4]. Bakshi, M.; Singh, S. Biomed. Anal. 2004, 34, 11-18.
https://doi.org/10.1016/j.japna.2003.08.003

[5]. Pai, P. N. S.; Rao, G. K.; Srinivas, B.; Puranik S. Indian J. Pharm. Sci. 2008, 70, 670-672.
https://doi.org/10.4103/0250-474X.45415

[6]. Abou-Taleb, N.; El-Sherbiny, D.; El-Wasseef, D.; Abu El-Enin, M.; El-Ashry, S. Int. J. Biomed. Sci. 2011, 7, 137-144.

[7]. International Conference on Harmonization Q2 (R1), Validation of analytical procedures: Text and methodology, ICH Harmonized Tripartite Guideline, 2005.

[8]. US Pharmacopeia (USP), General Information Chapter: Verification of Compendial Procedures, 1226, 2008.

[9]. Bakshi, M.; Singh. S. Pharm. Biome. Anal. 2002, 28, 1011-1040.
https://doi.org/10.1016/S0731-7085(02)00047-X

[10]. Basavaiah, K.; NageGowda, P.; Chandraashekar, U. Indian J. Chem. Technol. 2005, 12, 273-280.

[11]. Salo, P.; Salomies, H. Pharm. Biomed. Anal. 1996, 14, 1261-1266.
https://doi.org/10.1016/S0731-7085(96)01769-4

[12]. Sun, H. W.; Wang, F. C.; Ai, L. F. Chromatogr. B Analyt Technol. Biomed. Life Sci. 2007, 857, 296-300.
https://doi.org/10.1016/j.jchromb.2007.07.039

[13]. Sadana, G. S.; Ghogare, A. B. Indian Drugs 1990, 28, 145-148.

[14]. Patel, Y.; Dhorda, U. J.; Sundareesan, M.; Bhagawat, A. M. Anal. Chim. Acta 1998, 362, 271-277.
https://doi.org/10.1016/S0003-2670(98)00006-3

[15]. Yang, C. Anal. Sci. 2004, 20, 821-824.
https://doi.org/10.2116/analsci.20.821

[16]. Wang, C.; Wang, F.; Li, C.; Xu, X.; Li, T. Pharma Biomed. Anal. 2006, 41, 1396-1400.
https://doi.org/10.1016/j.jpba.2006.02.040

[17]. Salvi, V. S.; Sathe, P. A.; Rege P. V. Anal. Bioanal. Tech. 2010, 1, 1-3.

[18]. Zhang, L.; Zhang, Z.; Wu, K. Pharma Biomed. Anal. 2009, 41, 1453-1457.
https://doi.org/10.1016/j.jpba.2006.03.016

[19]. VRege, P.; Sathe, P. A.; Salvi, V. S. Asian J. Res. Chem. 2011, 4, 729-733.

[20]. Sharma, R.; Pathodiya, G.; Mishra, G. P.; Sainy, J. Pharmacy Res. 2011, 4, 859-861.

[21]. Chandorkar, J. G.; Pande, V. V.; Dhande, N. S.; Parashar, G. A. Inter. J. Pharm. Tech. Res. 2009, 1, 62-67.

[22]. Kamel, M. S.; Barsoum, B. N.; Rania, S. App. Sci. 2009, 5, 880-886.

[23]. Khaja, P.; Asgar, A.; Shahana, B.; Syeda, H. Int. J. Pharm. Pharm. Sci. 2010, 2, 46-47.

[24]. Anju, G.; Sweety, C.; Gaurav, S. Int. J. Chem. Pharm. Anal. 2015, 2, 22-27.

TrendMD

Dimensions - Altmetric - scite_ - PlumX

Downloads and views

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...
License Terms

License Terms

by-nc

Copyright © 2024 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at https://www.eurjchem.com/index.php/eurjchem/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (https://www.eurjchem.com/index.php/eurjchem/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).